DNA hybridization analysis of mycobacterial DNA using the 18-kDa protein gene of Mycobacterium leprae.

DNA hybridization studies using a 611-base pair (bp) probe, encoding the entire 18-kDa protein of Mycobacterium leprae, demonstrated that M. simiae, M. intracellulare, M. kansasii, M. terrae, ADM-2, M. avium, M. scrofulaceum, M. gordonae and M. chelonei appear to possess DNA sequences homologous to the 18-kDa protein gene of M. leprae. RFLP analysis revealed that the restriction sites in the M. leprae 18-kDa gene were not conserved in the putative gene homologs of M. simiae and M. intracellulare. The restriction patterns observed with the 611-bp probe were useful in differentiating M. intracellulare, M. simiae, and M. leprae from each other, as well as in distinguishing strains of M. simiae serovar 1. Finally, the presence of homologous sequences in various mycobacteria did not affect the specificity of a previously described PCR test for detection of M. leprae, based on the M. leprae 18-kDa protein gene.

[1]  R. Booth,et al.  The use of a specific DNA probe and polymerase chain reaction for the detection of Mycobacterium leprae. , 1990, The Journal of infectious diseases.

[2]  M. Colston,et al.  The specific 18-kilodalton antigen of Mycobacterium leprae is present in Mycobacterium habana and functions as a heat-shock protein. , 1990, Journal of immunology.

[3]  R. Booth,et al.  The mapping of epitopes of the 18-kDa protein of Mycobacterium leprae recognized by murine T cells in a proliferation assay. , 1989, Journal of immunology.

[4]  P. Klatser,et al.  Polymerase chain reaction for the detection of Mycobacterium leprae. , 1989, Journal of general microbiology.

[5]  H. Dockrell,et al.  T-cell recognition of the 18-kilodalton antigen of Mycobacterium leprae , 1989, Infection and immunity.

[6]  W. Jacobs,et al.  Genetic relationships among Mycobacterium leprae, Mycobacterium tuberculosis, and candidate leprosy vaccine strains determined by DNA hybridization: identification of an M. leprae-specific repetitive sequence , 1989, Infection and immunity.

[7]  H. Bercovier,et al.  Characterization and taxonomic implications of the rRNA genes of Mycobacterium leprae , 1989, Journal of bacteriology.

[8]  Williams Dl,et al.  A study of the relatedness of Mycobacterium leprae isolates using restriction fragment length polymorphism analysis. , 1989 .

[9]  R. Young,et al.  A protein antigen of Mycobacterium leprae is related to a family of small heat shock proteins , 1988, Journal of bacteriology.

[10]  A. Mustafa Identification of T-cell-activating recombinant antigens shared among three candidate antileprosy vaccines, killed M. leprae, M. bovis BCG, and mycobacterium w. , 1988, International journal of leprosy and other mycobacterial diseases : official organ of the International Leprosy Association.

[11]  R. Young,et al.  Stress proteins are immune targets in leprosy and tuberculosis. , 1988, Proceedings of the National Academy of Sciences of the United States of America.

[12]  R. Booth,et al.  Antigenic proteins of Mycobacterium leprae. Complete sequence of the gene for the 18-kDa protein. , 1988, Journal of immunology.

[13]  F. Portaels,et al.  A cooperative taxonomic study of mycobacteria isolated from armadillos infected with Mycobacterium leprae. , 1986, Journal of general microbiology.

[14]  T. Ottenhoff,et al.  Mycobacterium leprae-specific protein antigens defined by cloned human helper T cells , 1986, Nature.

[15]  R. Young,et al.  Human T-cell clones recognize a major M. leprae protein antigen expressed in E. coli , 1986, Nature.

[16]  A. Basten,et al.  Mycobacterium leprae antigens involved in human immune responses. I. Identification of four antigens by monoclonal antibodies. , 1985, Journal of immunology.

[17]  K. C. Reed,et al.  Rapid transfer of DNA from agarose gels to nylon membranes. , 1985, Nucleic acids research.

[18]  Ronald W. Davis,et al.  Genes for the major protein antigens of the leprosy parasite Mycobacterium leprae , 1985, Nature.

[19]  W. Jacobs,et al.  Molecular analysis of DNA and construction of genomic libraries of Mycobacterium leprae , 1985, Journal of bacteriology.

[20]  S. Halstead Selective primary health care: strategies for control of disease in the developing world. XI. Dengue. , 1984, Reviews of infectious diseases.

[21]  J. Sambrook,et al.  Molecular Cloning: A Laboratory Manual , 2001 .

[22]  T. Godal,et al.  Selective primary health care: strategies for control of disease in the developing world. V. Leprosy. , 1983, Reviews of infectious diseases.