Long-distance neuronal migration in the adult mammalian brain.

During the development of the mammalian brain, neuronal precursors migrate to their final destination from their site of birth in the ventricular and subventricular zones (VZ and SVZ, respectively). SVZ cells in the walls of the lateral ventricle continue to proliferate in the brain of adult mice and can generate neurons in vitro, but their fate in vivo is unknown. Here SVZ cells from adult mice that carry a neuronal-specific transgene were grafted into the brain of adult recipients. In addition, the fate of endogenous SVZ cells was examined by microinjection of tritiated thymidine or a vital dye that labeled a discrete population of SVZ cells. Grafted and endogenous SVZ cells in the lateral ventricle of adult mice migrate long distances and differentiate into neurons in the olfactory bulb.

[1]  P. Rakić.  Limits of neurogenesis in primates. , 1985, Science.

[2]  M. Nerenberg,et al.  Transgenic mice expressing β-galactosidase in mature neurons under neuron-specific enolase promoter control , 1990, Neuron.

[3]  P. Lantos,et al.  Distribution and fine structural analysis of undifferentiated cells in the primate subependymal layer. , 1991, Journal of anatomy.

[4]  J. Maruniak,et al.  Neurogenesis persists in the subependymal layer of the adult mouse brain , 1993, Neuroscience Letters.

[5]  S. Mcconnell,et al.  Diverse migratory pathways in the developing cerebral cortex. , 1992, Science.

[6]  S. Weiss,et al.  Generation of neurons and astrocytes from isolated cells of the adult mammalian central nervous system. , 1992, Science.

[7]  Maria B. Luskin,et al.  Restricted proliferation and migration of postnatally generated neurons derived from the forebrain subventricular zone , 1993, Neuron.

[8]  I. Smart.  The subependymal layer of the mouse brain and its cell production as shown by radioautography after thymidine‐H3 injection , 1961 .

[9]  J. Altman.  Autoradiographic and histological studies of postnatal neurogenesis. IV. Cell proliferation and migration in the anterior forebrain, with special reference to persisting neurogenesis in the olfactory bulb , 1969, The Journal of comparative neurology.

[10]  C. P. Leblond,et al.  The subependymal layer and neighboring region in the brain of the young rat , 1972, The Journal of comparative neurology.

[11]  K. Kishi.  Golgi studies on the development of granule cells of the rat olfactory bulb with reference to migration in the subependymal layer , 1987, The Journal of comparative neurology.

[12]  J. Hinds,et al.  Neurogenesis in the adult rat: electron microscopic analysis of light radioautographs. , 1977, Science.

[13]  Fernando Nottebohm,et al.  Migration of young neurons in adult avian brain , 1988, Nature.

[14]  J. Altman,et al.  Autoradiographic and histological evidence of postnatal hippocampal neurogenesis in rats , 1965, The Journal of comparative neurology.

[15]  W. Blakemore,et al.  The subependymal plate and associated ependyma in the dog. An ultrastructural study , 1972, Journal of neurocytology.

[16]  M. Miller,et al.  Bromodeoxyuridine immunohistochemical determination of the lengths of the cell cycle and the DNA-synthetic phase for an anatomically defined population , 1989, Journal of neurocytology.

[17]  S. Bayer,et al.  Neurons in the rat dentate gyrus granular layer substantially increase during juvenile and adult life. , 1982, Science.

[18]  Arturo Alvarez-Buylla,et al.  Simple microcomputer system for mapping tissue sections with the light microscope , 1988, Journal of Neuroscience Methods.

[19]  F. Nottebohm,et al.  Birth of projection neurons in adult avian brain may be related to perceptual or motor learning. , 1990, Science.

[20]  C. Cepko,et al.  Widespread dispersion of neuronal clones across functional regions of the cerebral cortex. , 1992, Science.

[21]  C. Lois,et al.  Proliferating subventricular zone cells in the adult mammalian forebrain can differentiate into neurons and glia. , 1993, Proceedings of the National Academy of Sciences of the United States of America.

[22]  G. Fishell,et al.  Dispersion of neural progenitors within the germinal zones of the forebrain , 1993, Nature.

[23]  D. Pfaff,et al.  RB and Cdc2 expression in brain: correlations with 3H-thymidine incorporation and neurogenesis , 1993, The Journal of neuroscience : the official journal of the Society for Neuroscience.

[24]  D. van der Kooy,et al.  Postmitotic death is the fate of constitutively proliferating cells in the subependymal layer of the adult mouse brain , 1992, The Journal of neuroscience : the official journal of the Society for Neuroscience.

[25]  F. Nottebohm,et al.  Neurons generated in the adult brain are recruited into functional circuits. , 1984, Science.

[26]  R. Skalko,et al.  Incorportaiton of thymidine and its analogue, bromodeoxyuridine, into embryos and maternal tissues of the mouse. , 1973, Differentiation; research in biological diversity.