Alginate Films as Macromolecular Imprinted Matrices

Macromolecularly imprinted polymers have been developed to mimic the non-covalent interactions driving molecular recognition in nature. The creation of an engineered antibody mimic would allow for the development of customizable films for biomolecular sensing. To demonstrate this principle, a cross-linked alginate film has been imprinted with bovine serum albumin (BSA) using aqueous biocompatible gelation methods. The imprinting efficiency of the synthesized films imprinted with BSA was determined and compared to the non-specific uptake of complementary proteins which were not imprinted in the alginate matrix. It was found that the recognition of the BSA using an alginate film was 6.4 mg/g polymer, which compares favorably to previously reported macromolecularly imprinted networks. The absorption of non-imprinted cationic proteins by the alginate matrix demonstrates that overcoming non-specific binding needs to be a focus of future work in order to successfully employ these materials towards biomolecular sensing within a physiological environment.

[1]  Xiaoguang Ying,et al.  Emulsion and macromolecules templated alginate based polymer microspheres , 2006 .

[2]  Nicholas A. Peppas,et al.  Molecularly imprinted polymers with specific recognition for macromolecules and proteins , 2008 .

[3]  David J Mooney,et al.  Alginate hydrogels as biomaterials. , 2006, Macromolecular bioscience.

[4]  Wee,et al.  Protein release from alginate matrices. , 1998, Advanced drug delivery reviews.

[5]  L. Ye,et al.  Molecularly imprinted microspheres as antibody binding mimics , 2001 .

[6]  Xiaoguang Ying,et al.  Macromolecularly Imprinted Calcium Phosphate/ Alginate Hybrid Polymer Microspheres with the Surface Imprinting of Bovine Serum Albumin in Inverse-Phase Suspension , 2008 .

[7]  K. Mosbach,et al.  Synthesis of substrate‐selective polymers by host‐guest polymerization , 1981 .

[8]  K. Shea,et al.  Selective protein capture by epitope imprinting. , 2006, Angewandte Chemie.

[9]  Shunqing Tang,et al.  Protein recognition via molecularly imprinted agarose gel membrane. , 2008, Journal of biomedical materials research. Part A.

[10]  D. Hansen,et al.  Recent developments in the molecular imprinting of proteins. , 2007, Biomaterials.

[11]  H. Tønnesen,et al.  Alginate in Drug Delivery Systems , 2002, Drug development and industrial pharmacy.

[12]  Allan S Hoffman,et al.  Hydrogels for biomedical applications. , 2002, Advanced drug delivery reviews.

[13]  Junfu Wei,et al.  Preparation of bovine serum albumin‐imprinted calcium polyacrylate/alginate hybrid microspheres via Ca2+ crosslinking , 2009 .

[14]  Nicholas A Peppas,et al.  Mimicking Biological Delivery Through Feedback-Controlled Drug Release Systems Based on Molecular Imprinting. , 2009, AIChE journal. American Institute of Chemical Engineers.

[15]  Mark E Byrne,et al.  Molecular imprinting within hydrogels II: progress and analysis of the field. , 2008, International journal of pharmaceutics.

[16]  K. Felgenhauer.  Protein size and cerebrospinal fluid composition , 1974, Klinische Wochenschrift.

[17]  Sergey Piletsky,et al.  Surface imprinted beads for the recognition of human serum albumin. , 2007, Biosensors & bioelectronics.

[18]  T. Chou,et al.  Albumin molecularly imprinted polymer with high template affinity — Prepared by systematic optimization in mixed organic/aqueous media , 2009 .

[19]  John O'Mahony,et al.  Molecular imprinting science and technology: a survey of the literature for the years up to and including 2003 , 2006, Journal of molecular recognition : JMR.

[20]  A. Mikos,et al.  Review: Hydrogels for cell immobilization , 2000, Biotechnology and bioengineering.

[21]  Xiaoguang Ying,et al.  Rebinding and recognition properties of protein-macromolecularly imprinted calcium phosphate/alginate hybrid polymer microspheres , 2008 .

[22]  Klaus Mosbach,et al.  Highly enantioselective and substrate-selective polymers obtained by molecular imprinting utilizing noncovalent interactions. NMR and chromatographic studies on the nature of recognition , 1988 .

[23]  Lei Ye,et al.  Molecular imprinting: Synthetic materials as substitutes for biological antibodies and receptors , 2008 .

[24]  J. Z. Hilt,et al.  Configurational biomimesis in drug delivery: molecular imprinting of biologically significant molecules. , 2004, Advanced drug delivery reviews.

[25]  Carolyn L. Bayer,et al.  Advances in recognitive, conductive and responsive delivery systems. , 2008, Journal of controlled release : official journal of the Controlled Release Society.

[26]  Klaus Mosbach,et al.  Molecular imprinting of amino acid derivatives at low temperature (0°C) using photolytic homolysis of azobisnitriles , 1989 .