ENHANCEMENT OF A NEW METHOD IN CEREAL BREEDING PROGRAMMES

Homozygous doubled haploid (DH) plants and lines can be used in cereal breeding programmes. Methods based on androgenesis induction are a common way to produce homogenous basic material for variety development and genetic research purposes. Anther culture is a simple and rapid method for DH production in case of major cereal crops like barley and wheat. Oat is known as recalcitrant species for tissue culture response specially anther and microspore culture. Its low frequency DH production limits extensive application in breeding. Our aim was to start developing an improved protocol to generate acceptable number of DH lines for breeding. Many factors (low induction rate, essential manpower needed, plant regeneration problems and genotype dependence) hinder the development and application of the methods of in vitro androgenesis. Understanding of topic “in vitro response” and “plantlet regeneration frequency” are crucial factors in cereal science, too. Our aimed results of oat (Avena sativa L.) will open new genetic solutions in plant science, plant physiology and celland tissue culture of cereals and in the development of new

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