Tissue engineered artificial skin composed of dermis and epidermis.

We made an artificial skin comprised of a stratified layer of keratinocytes and a dermal matrix with a type I collagen containing fibroblasts. In this work, we showed keratinocyte behavior under primary culture, gel contractions varying with concentration of collagen solution, and cell growth plots in the collagen gel. The optimum behavior of dermal equivalent could be obtained using 3.0 mg/ml collagen solution and attached gel culture. The attached gel culture had a jumping effect of growth factor on cell growth at the lag phase. To develop the artificial skin, 1x10(5) cells/cm2 of keratinocytes were cultured on the dermal equivalent at air-liquid interface. Finally, to overcome the problem that artificial skin of collagen gel was torn easily during suturing of grafting, we prepared histocompatible collagen mesh and attached the mesh to the bottom of the gel. Cultured artificial skins were successfully grafted onto rats.

[1]  H Green,et al.  Growth of cultured human epidermal cells into multiple epithelia suitable for grafting. , 1979, Proceedings of the National Academy of Sciences of the United States of America.

[2]  E Bell,et al.  Production of a tissue-like structure by contraction of collagen lattices by human fibroblasts of different proliferative potential in vitro. , 1979, Proceedings of the National Academy of Sciences of the United States of America.

[3]  I. Yannas,et al.  Design of an artificial skin. II. Control of chemical composition. , 1980, Journal of biomedical materials research.

[4]  S. Yuspa,et al.  Optimized conditions for the growth of human epidermal cells in culture. , 1980, The Journal of investigative dermatology.

[5]  JOHN F. BURKE,et al.  Successful Use of a Physiologically Acceptable Artificial Skin in the Treatment of Extensive Burn Injury , 1981, Annals of surgery.

[6]  E Bell,et al.  Living tissue formed in vitro and accepted as skin-equivalent tissue of full thickness. , 1981, Science.

[7]  I. Yannas,et al.  Wound tissue can utilize a polymeric template to synthesize a functional extension of skin. , 1982, Science.

[8]  B. Hull,et al.  Structural integration of skin equivalents grafted to Lewis and Sprague-Dawley rats. , 1983, The Journal of investigative dermatology.

[9]  I. Cooke,et al.  Immunocytochemically identified vasopressin neurons in culture show slow, calcium-dependent electrical responses. , 1983, Science.

[10]  H. Ehrlich,et al.  Fibroblast contraction of collagen lattices in vitro: Inhibition by chronic inflammatory cell mediators , 1983, Journal of cellular physiology.

[11]  B. Nusgens,et al.  Collagen biosynthesis by cells in a tissue equivalent matrix in vitro. , 1984, Collagen and related research.

[12]  L. Dubertret,et al.  Psoriatic fibroblasts induce hyperproliferation of normal keratinocytes in a skin equivalent model in vitro. , 1985, Science.

[13]  M. Pittelkow,et al.  Two functionally distinct classes of growth arrest states in human prokeratinocytes that regulate clonogenic potential. , 1986, The Journal of investigative dermatology.

[14]  F. Grinnell,et al.  Extracellular matrix organization modulates fibroblast growth and growth factor responsiveness. , 1989, Experimental cell research.

[15]  E. Fuchs,et al.  Epidermal differentiation: the bare essentials , 1990, The Journal of cell biology.

[16]  L. Dubertret,et al.  Activities of human acidic fibroblast growth factor in an in vitro dermal equivalent model. , 1991, The Journal of investigative dermatology.

[17]  J. Vacanti,et al.  Tissue engineering : Frontiers in biotechnology , 1993 .

[18]  K. Kraus,et al.  Collagen fabrics as biomaterials , 1994, Biotechnology and bioengineering.

[19]  M. Auletta,et al.  Cultured skin as a 'smart material' for healing wounds: experience in venous ulcers. , 1996, Biomaterials.

[20]  Contraction behavior of collagen gel and fibroblasts activity in dermal equivalent model , 1997 .

[21]  G. Naughton,et al.  A metabolically active human dermal replacement for the treatment of diabetic foot ulcers. , 2008, Artificial organs.

[22]  Construction of artificial epithelial tissues prepared from human normal fibroblasts and C9 cervical epithelial cancer cells carrying human papillomavirus type 18 genes , 1998 .