Effect of Hot- and Cold-Water Treatment on Broccoli Bioactive Compounds, Oxidative Stress Parameters and Biological Effects of Their Extracts

The goal of this work was to define resistant and susceptible variables of young broccoli (Brassica oleracea L. convar. botrytis (L.) Alef. var. cymosa Duch.) plants treated with cold and hot water. Additionally, we wanted to single out variables that could potentially be used as biomarkers of cold/hot-water stress in broccoli. Hot water changed more variables (72%) of young broccoli than cold water (24%) treatment. Hot water increased the concentration of vitamin C for 33%, hydrogen peroxide for 10%, malondialdehyde for 28%, and proline for 147%. Extracts of broccoli stressed with hot water were significantly more efficient in the inhibition of α-glucosidase (65.85 ± 4.85% compared to 52.00 ± 5.16% of control plants), while those of cold-water-stressed broccoli were more efficient in the inhibition of α-amylase (19.85 ± 2.70% compared to 13.26 ± 2.36% of control plants). Total glucosinolates and soluble sugars were affected by hot and cold water in an opposite way, which is why they could be used as biomarkers of hot/cold-water stress in broccoli. The possibility of using temperature stress to grow broccoli enriched with compounds of interest to human health should be further investigated.

[1]  C. Nobre,et al.  Effect of Gastrointestinal Digestion on the Bioaccessibility of Phenolic Compounds and Antioxidant Activity of Fermented Aloe vera Juices , 2022, Antioxidants.

[2]  A. Magiera,et al.  The Inhibition of α-Glucosidase, α-Amylase and Protein Glycation by Phenolic Extracts of Cotoneaster bullatus, Cotoneaster zabelii, and Cotoneaster integerrimus Leaves and Fruits: Focus on Anti-Hyperglycemic Activity and Kinetic Parameters , 2022, Molecules.

[3]  A. Gęgotek,et al.  Antioxidative and Anti-Inflammatory Activity of Ascorbic Acid , 2022, Antioxidants.

[4]  Ananda Kulal,et al.  Investigation of effective natural inhibitors for starch hydrolysing enzymes from Simaroubaceae plants by molecular docking analysis and comparison with in-vitro studies , 2022, Heliyon.

[5]  T. Hyun,et al.  Plant Response to Cold Stress: Cold Stress Changes Antioxidant Metabolism in Heading Type Kimchi Cabbage (Brassica rapa L. ssp. Pekinensis) , 2022, Antioxidants.

[6]  B. Hawkins,et al.  Condensed tannins as antioxidants that protect poplar against oxidative stress from drought and UV-B. , 2021, Plant, cell & environment.

[7]  A. Piccolo,et al.  Valorisation of lignins from energy crops and agro-industrial byproducts as antioxidant and antibacterial materials. , 2021, Journal of the science of food and agriculture.

[8]  L. Brkljacic,et al.  Ferulic Acid and Salicylic Acid Foliar Treatments Reduce Short-Term Salt Stress in Chinese Cabbage by Increasing Phenolic Compounds Accumulation and Photosynthetic Performance , 2021, Plants.

[9]  D. Šamec,et al.  Effects of Short-Term Exposure to Low Temperatures on Proline, Pigments, and Phytochemicals Level in Kale (Brassica oleracea var. acephala) , 2021, Horticulturae.

[10]  J. Crossa,et al.  Drought and Heat Stress Impacts on Phenolic Acids Accumulation in Durum Wheat Cultivars , 2021, Foods.

[11]  T. Tschaplinski,et al.  Role of Reactive Oxygen Species and Hormones in Plant Responses to Temperature Changes , 2021, International journal of molecular sciences.

[12]  Zhang Lele,et al.  Cold Stress in Wheat: Plant Acclimation Responses and Management Strategies , 2021, Frontiers in Plant Science.

[13]  H. Alhaithloul,et al.  Effect of extreme temperature changes on phenolic, flavonoid contents and antioxidant activity of tomato seedlings (Solanum lycopersicum L.) , 2021, PeerJ.

[14]  G. Rusak,et al.  Effect of flooding and drought on the content of phenolics, sugars, photosynthetic pigments and vitamin C, and antioxidant potential of young Chinese cabbage , 2021, European Food Research and Technology.

[15]  G. Rusak,et al.  Matcha and Sencha green tea extracts with regard to their phenolics pattern and antioxidant and antidiabetic activity during in vitro digestion , 2021, Journal of Food Science and Technology.

[16]  M. Fujita,et al.  Abiotic Stress and Reactive Oxygen Species: Generation, Signaling, and Defense Mechanisms , 2021, Antioxidants.

[17]  G. Rusak,et al.  Improving the phytochemical profile and bioactivity of Chinese cabbage sprouts by interspecific transfer of metabolites. , 2020, Food research international.

[18]  G. Ren,et al.  Changes in bio‐accessibility, polyphenol profile and antioxidants of quinoa and djulis sprouts during in vitro simulated gastrointestinal digestion , 2020, Food science & nutrition.

[19]  N. Goto-Yamamoto,et al.  Evaluating the influence of temperature on proanthocyanidin biosynthesis in developing grape berries (Vitis vinifera L.) , 2020, Molecular Biology Reports.

[20]  Pooja Shah,et al.  Comparative Study of DPPH, ABTS and FRAP Assays for Determination of Antioxidant Activity , 2020 .

[21]  E. Llorent-Martínez,et al.  Inhibition of α-amylase, α-glucosidase and pancreatic lipase by phenolic compounds of Rumex maderensis (Madeira sorrel). Influence of simulated gastrointestinal digestion on hyperglycaemia-related damage linked with aldose reductase activity and protein glycation , 2020 .

[22]  G. Rusak,et al.  Developmentally-related changes in phenolic and L-ascorbic acid content and antioxidant capacity of Chinese cabbage sprouts , 2020, Journal of Food Science and Technology.

[23]  S. Jang,et al.  Alpha Glucosidase Inhibitory Activities of Plants with Focus on Common Vegetables , 2019, Plants.

[24]  K. Shimizu,et al.  White poplar: Targeted isolation of pancreatic lipase inhibitors , 2019 .

[25]  C. Kaya,et al.  Potential Mechanisms of Abiotic Stress Tolerance in Crop Plants Induced by Thiourea , 2019, Front. Plant Sci..

[26]  Colleen J. Doherty,et al.  Only a matter of time: the impact of daily and seasonal rhythms on phytochemicals , 2019, Phytochemistry Reviews.

[27]  Hafiz Ansar Rasul Suleria,et al.  Proanthocyanidins: A comprehensive review. , 2019, Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie.

[28]  Bingru Huang,et al.  Differential Heat-Induced Changes in Phenolic Acids Associated with Genotypic Variations in Heat Tolerance for Hard Fescue , 2019, Crop Science.

[29]  S. Harding.  Condensed tannins: arbiters of abiotic stress tolerance? , 2019, Tree physiology.

[30]  M. Chorilli,et al.  A Review of Analytical Methods for p-Coumaric Acid in Plant-Based Products, Beverages, and Biological Matrices , 2019, Critical reviews in analytical chemistry.

[31]  Cheng-qiang He,et al.  Abiotic Stresses: General Defenses of Land Plants and Chances for Engineering Multistress Tolerance , 2018, Front. Plant Sci..

[32]  A. Mollica,et al.  Polyphenolic composition, enzyme inhibitory effects ex-vivo and in-vivo studies on two Brassicaceae of north-central Italy. , 2018, Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie.

[33]  G. Rusak,et al.  Comparative Analysis of Native Crocus Taxa as a Great Source of Flavonoids with High Antioxidant Activity , 2018, Plant Foods for Human Nutrition.

[34]  M. E. Cartea,et al.  Effect of Temperature Stress on Antioxidant Defenses in Brassica oleracea , 2018, ACS omega.

[35]  P. Paśko,et al.  Comparative Study of Predominant Phytochemical Compounds and Proapoptotic Potential of Broccoli Sprouts and Florets , 2018, Plant Foods for Human Nutrition.

[36]  G. Prinsloo,et al.  The effects of season and water availability on chemical composition, secondary metabolites and biological activity in plants , 2018, Phytochemistry Reviews.

[37]  Ke Chen,et al.  Ascorbic Acid Alleviates Damage from Heat Stress in the Photosystem II of Tall Fescue in Both the Photochemical and Thermal Phases , 2017, Front. Plant Sci..

[38]  J. Dukes,et al.  Climate Influences the Content and Chemical Composition of Foliar Tannins in Green and Senesced Tissues of Quercus rubra , 2017, Front. Plant Sci..

[39]  B. Gurung,et al.  A simple spectrophotometric method for estimating total glucosinolates in mustard de-oiled cake , 2017 .

[40]  J. Yadav,et al.  Effect of climate change on phytochemical diversity, total phenolic content and in vitro antioxidant activity of Aloe vera (L.) Burm.f. , 2017, BMC Research Notes.

[41]  A. Khan,et al.  HEAT SHOCK INDUCED MORPHO-PHYSIOLOGICAL RESPONSE IN INDICA RICE (ORYZA SATIVA L.) AT EARLY SEEDLING STAGE , 2017 .

[42]  A. Faraz,et al.  Role of sugars under abiotic stress. , 2016, Plant physiology and biochemistry : PPB.

[43]  F. Romeo,et al.  Screening of the anthocyanin profile and in vitro pancreatic lipase inhibition by anthocyanin-containing extracts of fruits, vegetables, legumes and cereals. , 2016, Journal of the science of food and agriculture.

[44]  C. Pieterse,et al.  Recognizing Plant Defense Priming. , 2016, Trends in plant science.

[45]  S. Ou,et al.  p-Coumaric acid and its conjugates: dietary sources, pharmacokinetic properties and biological activities. , 2016, Journal of the science of food and agriculture.

[46]  Sushil Kumar,et al.  Effect of short-term heat stress on total sugars, proline and some antioxidant enzymes in moth bean (Vigna aconitifolia) , 2016 .

[47]  J. Juvik,et al.  The Role of Glucosinolate Hydrolysis Products from Brassica Vegetable Consumption in Inducing Antioxidant Activity and Reducing Cancer Incidence , 2016, Diseases.

[48]  G. Rimbach,et al.  Metabolic Activity of Radish Sprouts Derived Isothiocyanates in Drosophila melanogaster , 2016, International journal of molecular sciences.

[49]  V. Thoss,et al.  The Diversity of Phenolic Compounds along Defatted Kernel, Green Husk and Leaves of Walnut (Juglansregia L.) , 2016 .

[50]  Maressa Caldeira Morzelle,et al.  Antioxidant and antiproliferative activities in different maturation stages of broccoli (Brassica oleracea Italica) biofortified with selenium. , 2016, Food chemistry.

[51]  S. Rivas-Arancibia,et al.  Vitamin C in Health and Disease: Its Role in the Metabolism of Cells and Redox State in the Brain , 2015, Front. Physiol..

[52]  Iwona Majewska,et al.  Antidiabetic effect of polyphenolic extracts from selected edible plants as α-amylase, α -glucosidase and PTP1B inhibitors, and β pancreatic cells cytoprotective agents - a comparative study. , 2015, Current topics in medicinal chemistry.

[53]  M. Alshagga,et al.  Medicinal Plants and Their Inhibitory Activities against Pancreatic Lipase: A Review , 2015, Evidence-based complementary and alternative medicine : eCAM.

[54]  Mitsuru Watanabe,et al.  Effect of low temperature on flavonoids, oxygen radical absorbance capacity values and major components of winter sweet spinach (Spinacia oleracea L.). , 2015, Journal of the science of food and agriculture.

[55]  E. Hansen,et al.  Effects of photoperiod, growth temperature and cold acclimatisation on glucosinolates, sugars and fatty acids in kale. , 2015, Food chemistry.

[56]  Ł. Tarkowski,et al.  Cold tolerance triggered by soluble sugars: a multifaceted countermeasure , 2015, Front. Plant Sci..

[57]  H. Nayyar,et al.  Temperature stress and redox homeostasis in agricultural crops , 2015, Front. Environ. Sci..

[58]  S. Bhandari,et al.  Chemical Composition and Antioxidant Activity in Different Tissues of Brassica Vegetables , 2015, Molecules.

[59]  L. Urban,et al.  Optimized Assay for Hydrogen Peroxide Determination in Plant Tissue Using Potassium Iodide , 2014 .

[60]  D. Šamec,et al.  Assessing the authenticity of the white cabbage (Brassica oleracea var. capitata f. alba) cv. ‘Varaždinski’ by molecular and phytochemical markers , 2014 .

[61]  Iwona Majewska,et al.  In vitro inhibitory effect on digestive enzymes and antioxidant potential of commonly consumed fruits. , 2014, Journal of agricultural and food chemistry.

[62]  R. Amarowicz,et al.  Changes in the composition of phenolic compounds and antioxidant properties of grapevine roots and leaves (Vitis vinifera L.) under continuous of long-term drought stress , 2014, Acta Physiologiae Plantarum.

[63]  N. Baenas,et al.  Evaluation of Latin-American fruits rich in phytochemicals with biological effects , 2014 .

[64]  Nayek Sumanta,et al.  Spectrophotometric Analysis of Chlorophylls and Carotenoids from Commonly Grown Fern Species by Using Various Extracting Solvents , 2014 .

[65]  Praveen Sharma,et al.  Vitamin C in Disease Prevention and Cure: An Overview , 2013, Indian Journal of Clinical Biochemistry.

[66]  M. Carvajal,et al.  The Physiological Importance of Glucosinolates on Plant Response to Abiotic Stress in Brassica , 2013, International journal of molecular sciences.

[67]  Md. Mahabubul Alam,et al.  Physiological, Biochemical, and Molecular Mechanisms of Heat Stress Tolerance in Plants , 2013, International journal of molecular sciences.

[68]  Kirill S. Mironov,et al.  Regulatory role of membrane fluidity in gene expression and physiological functions , 2013, Photosynthesis Research.

[69]  A. F. Abdull Razis,et al.  Cruciferous vegetables: dietary phytochemicals for cancer prevention. , 2013, Asian Pacific journal of cancer prevention : APJCP.

[70]  V. R. Devaraj,et al.  Induction of thermotolerance through heat acclimation in lablab bean ( Dolichos lablab ) , 2013 .

[71]  P. Perata,et al.  ROS signaling as common element in low oxygen and heat stresses. , 2012, Plant physiology and biochemistry : PPB.

[72]  Heather Knight,et al.  Low-temperature perception leading to gene expression and cold tolerance in higher plants. , 2012, The New phytologist.

[73]  M. E. Otegui,et al.  Heat stress in temperate and tropical maize hybrids: Differences in crop growth, biomass partitioning and reserves use , 2012 .

[74]  L. A. Simeoni,et al.  Inhibitory activity of α-amylase and α-glucosidase by plant extracts from the Brazilian cerrado. , 2012, Planta medica.

[75]  L. A. Simeoni,et al.  α-Amylase inhibitors: a review of raw material and isolated compounds from plant source. , 2012, Journal of pharmacy & pharmaceutical sciences : a publication of the Canadian Society for Pharmaceutical Sciences, Societe canadienne des sciences pharmaceutiques.

[76]  C. Jonak,et al.  Drought, salt, and temperature stress-induced metabolic rearrangements and regulatory networks. , 2012, Journal of experimental botany.

[77]  P. Njoku,et al.  Temperature Effects on Vitamin C Content in Citrus Fruits , 2011 .

[78]  K. Shinozaki,et al.  Effects of abiotic stress on plants: a systems biology perspective , 2011, BMC Plant Biology.

[79]  H. Ahmad,et al.  Relationships between Obesity and Cardiovascular Diseases in Four Southern States and Colorado , 2011, Journal of health care for the poor and underserved.

[80]  Dae-Ok Kim,et al.  Comparison of ABTS/DPPH assays to measure antioxidant capacity in popular antioxidant-rich US foods , 2011 .

[81]  A. Pathmeswaran,et al.  Soil-Transmitted Helminth Infections among Plantation Sector Schoolchildren in Sri Lanka: Prevalence after Ten Years of Preventive Chemotherapy , 2011, PLoS neglected tropical diseases.

[82]  J. Ovesná,et al.  Cold stress and acclimation - what is important for metabolic adjustment? , 2010, Plant biology.

[83]  R. Tundis,et al.  Natural Products as α-Amylase and α-Glucosidase Inhibitors and their Hypoglycaemic Potential in the Treatment of Diabetes: An Update , 2010 .

[84]  M. Carvajal,et al.  The response of broccoli plants to high temperature and possible role of root aquaporins. , 2010 .

[85]  R. Suwa,et al.  High temperature effects on photosynthate partitioning and sugar metabolism during ear expansion in maize (Zea mays L.) genotypes. , 2010, Plant physiology and biochemistry : PPB.

[86]  B. Efeoğlu,et al.  Photosynthetic responses of two wheat varieties to high temperature , 2009 .

[87]  M. Semenov,et al.  Identifying target traits and molecular mechanisms for wheat breeding under a changing climate. , 2009, Journal of experimental botany.

[88]  Shao Hongbo,et al.  Roles of plant soluble sugars and their responses to plant cold stress , 2009 .

[89]  J. González,et al.  Soluble sugars , 2009, Plant signaling & behavior.

[90]  Kyoung-Chan Park,et al.  Inhibitory effect of p-coumaric acid by Rhodiola sachalinensis on melanin synthesis in B16F10 cells. , 2008, Die Pharmazie.

[91]  B. P. Naidu,et al.  Compatible solute accumulation and stress-mitigating effects in barley genotypes contrasting in their salt tolerance. , 2007, Journal of experimental botany.

[92]  G. Balogh,et al.  Regulation of desaturase gene expression, changes in membrane lipid composition and freezing tolerance in potato plants , 2007, Molecular Breeding.

[93]  R. Yordanova,et al.  EFFECT OF EXOGENOUS TREATMENT WITH SALICYLIC ACID ON PHOTOSYNTHETIC ACTIVITY AND ANTIOXIDANT CAPACITY OF CHILLED WHEAT PLANTS , 2007 .

[94]  M. Miura,et al.  Inhibitory Effect of Vegetables, Fruits and Herbs on α-Glucosidase in an Immobilized Enzyme Assay System , 2006 .

[95]  Ruud Verkerk,et al.  Thermal degradation of glucosinolates in red cabbage , 2006 .

[96]  N. Suzuki,et al.  Reactive oxygen species and temperature stresses: A delicate balance between signaling and destruction , 2006 .

[97]  S. Adisakwattana,et al.  Structure–activity relationships of trans-cinnamic acid derivatives on α-glucosidase inhibition , 2004 .

[98]  S. Peikin,et al.  Orlistat: its current status as an anti-obesity drug. , 2002, European journal of pharmacology.

[99]  C. Rajashekar.  Cold Response and Freezing Tolerance in Plants , 2000 .

[100]  Mengcheng Tang,et al.  The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals , 1999 .

[101]  Kumar Tewari A,et al.  Temperature-stress-induced impairment of chlorophyll biosynthetic reactions in cucumber and wheat , 1998, Plant physiology.

[102]  J. Evans.  Straightforward Statistics for the Behavioral Sciences , 1995 .

[103]  S. S. Chen,et al.  Study of Microwave Extraction of Essential Oil Constituents from Plant Materials , 1994 .