Drug-loaded fluorescent cubosomes: versatile nanoparticles for potential theranostic applications.

In this work, monoolein-based cubosomes were doped with two fluorescent probes, namely, fluorescein and dansyl, properly modified with a hydrocarbon chain to increase their encapsulation efficiency within the monoolein palisade. The same nanocarriers were also loaded with quercetin, a hydrophobic molecule with potential anticancer activity. Particularly, the cubosomes doped with the modified fluorescein probe were successfully exploited for single living cell imaging. The physicochemical and photophysical characterizations reported here, along with the well-known ability of cubosomes in hosting molecules with pharmaceutical interest, strongly encourage the use of these innovative fluorescent nanocarriers for theranostic purposes.

[1]  Fabian Kiessling,et al.  Theranostic nanomedicine. , 2020, Accounts of chemical research.

[2]  M. Monduzzi,et al.  Physicochemical, Cytotoxic, and Dermal Release Features of a Novel Cationic Liposome Nanocarrier , 2013, Advanced healthcare materials.

[3]  S. Mohapatra,et al.  Manganese-loaded lipid-micellar theranostics for simultaneous drug and gene delivery to lungs. , 2013, Journal of controlled release : official journal of the Controlled Release Society.

[4]  R. Zhao,et al.  Engineering the Assemblies of Biomaterial Nanocarriers for Delivery of Multiple Theranostic Agents with Enhanced Antitumor Efficacy , 2013, Advances in Materials.

[5]  T. Maekawa,et al.  Multifunctional carboxymethyl cellulose-based magnetic nanovector as a theragnostic system for folate receptor targeted chemotherapy, imaging, and hyperthermia against cancer. , 2013, Langmuir : the ACS journal of surfaces and colloids.

[6]  Sonke Svenson,et al.  Theranostics: are we there yet? , 2013, Molecular pharmaceutics.

[7]  Nicholas A Peppas,et al.  Theranostic agents for intracellular gene delivery with spatiotemporal imaging. , 2013, Nano today.

[8]  M. Monduzzi,et al.  Characterization of the Solutol® HS15/water phase diagram and the impact of the Δ9-tetrahydrocannabinol solubilization. , 2013, Journal of colloid and interface science.

[9]  Yuquan Wei,et al.  Anticancer effect and mechanism of polymer micelle-encapsulated quercetin on ovarian cancer. , 2012, Nanoscale.

[10]  Kwangmeyung Kim,et al.  Tumor-targeting multi-functional nanoparticles for theragnosis: new paradigm for cancer therapy. , 2012, Advanced drug delivery reviews.

[11]  Beom Suk Lee,et al.  Theranostic nanoparticles based on PEGylated hyaluronic acid for the diagnosis, therapy and monitoring of colon cancer. , 2012, Biomaterials.

[12]  Frank Caruso,et al.  Engineering particles for therapeutic delivery: prospects and challenges. , 2012, ACS nano.

[13]  Si-Shen Feng,et al.  Theranostic liposomes of TPGS coating for targeted co-delivery of docetaxel and quantum dots. , 2012, Biomaterials.

[14]  Juan L. Vivero-Escoto,et al.  Silica-based nanoprobes for biomedical imaging and theranostic applications. , 2012, Chemical Society reviews.

[15]  F. Besenbacher,et al.  Enhancement of biological activities of nanostructured hydrophobic drug species. , 2012, Nanoscale.

[16]  Ki Young Choi,et al.  Theranostic nanoplatforms for simultaneous cancer imaging and therapy: current approaches and future perspectives. , 2012, Nanoscale.

[17]  Theresa M Reineke,et al.  Theranostics: combining imaging and therapy. , 2011, Bioconjugate chemistry.

[18]  A. Fadda,et al.  Effect of Penetration Enhancer Containing Vesicles on the Percutaneous Delivery of Quercetin through New Born Pig Skin , 2011, Pharmaceutics.

[19]  M. Monduzzi,et al.  In vitro release of lysozyme from gelatin microspheres: effect of cross-linking agents and thermoreversible gel as suspending medium. , 2011, Biomacromolecules.

[20]  C. Drummond,et al.  Steric stabilisation of self-assembled cubic lyotropic liquid crystalline nanoparticles: high throughput evaluation of triblock polyethylene oxide-polypropylene oxide-polyethylene oxide copolymers , 2011 .

[21]  Chantelle D. Driever,et al.  Converging layer-by-layer polyelectrolyte microcapsule and cubic lyotropic liquid crystalline nanoparticle approaches for molecular encapsulation , 2011 .

[22]  Leone Spiccia,et al.  Nanomaterials: Applications in Cancer Imaging and Therapy , 2011, Advanced materials.

[23]  G. Zhai,et al.  Lyotropic liquid crystal systems in drug delivery. , 2010, Drug discovery today.

[24]  M. Monduzzi,et al.  Nucleotide recognition and phosphate linkage hydrolysis at a lipid cubic interface. , 2010, Journal of the American Chemical Society.

[25]  A. S. Moses,et al.  Imaging and drug delivery using theranostic nanoparticles. , 2010, Advanced drug delivery reviews.

[26]  Jin Xie,et al.  Nanoparticle-based theranostic agents. , 2010, Advanced drug delivery reviews.

[27]  M. Giacca,et al.  Nanoparticles from lipid-based liquid crystals: emulsifier influence on morphology and cytotoxicity. , 2010, The journal of physical chemistry. B.

[28]  Mauro Ferrari,et al.  Nanomedicine—Challenge and Perspectives , 2009 .

[29]  O. Svensson,et al.  Interactions between drug delivery particles and mucin in solution and at interfaces. , 2008, Langmuir : the ACS journal of surfaces and colloids.

[30]  L. Prodi,et al.  Self-organizing core-shell nanostructures: spontaneous accumulation of dye in the core of doped silica nanoparticles. , 2007, Journal of the American Chemical Society.

[31]  Cui Tang,et al.  Chitosan graft copolymer nanoparticles for oral protein drug delivery: preparation and characterization. , 2006, Biomacromolecules.

[32]  Martin Malmsten,et al.  Soft drug delivery systems. , 2006, Soft matter.

[33]  P. Baglioni,et al.  Molecular recognition and controlled release in drug delivery systems based on nanostructured lipid surfactants , 2006 .

[34]  L. Prodi,et al.  Size effect on the fluorescence properties of dansyl-doped silica nanoparticles. , 2006, Langmuir : the ACS journal of surfaces and colloids.

[35]  A. Credi,et al.  Handbook of Photochemistry , 2006 .

[36]  M. Adrian,et al.  Crystallography of dispersed liquid crystalline phases studied by cryo‐transmission electron microscopy , 2006, Journal of microscopy.

[37]  M. Caffrey,et al.  Controlling release from the lipidic cubic phase. Amino acids, peptides, proteins and nucleic acids. , 2005, Journal of controlled release : official journal of the Controlled Release Society.

[38]  F. Tiberg,et al.  Cubic phases and cubic phase dispersions in a phospholipid-based system. , 2005, Journal of the American Chemical Society.

[39]  U. Olsson,et al.  Biocompatible lecithin organogels: structure and phase equilibria. , 2005, Langmuir : the ACS journal of surfaces and colloids.

[40]  Ignacy Gryczynski,et al.  Release of the self-quenching of fluorescence near silver metallic surfaces. , 2003, Analytical biochemistry.

[41]  T. Nylander,et al.  Effect of lipase on monoolein-based cubic phase dispersion (cubosomes) and vesicles , 2002 .

[42]  Allan Svendsen,et al.  Lipase action on a monoolein/sodium oleate aqueous cubic liquid crystalline phase—a NMR and X-ray diffraction study , 2002 .

[43]  M. Lynch,et al.  Novel Process for Producing Cubic Liquid Crystalline Nanoparticles (Cubosomes) , 2001 .

[44]  M. Monduzzi,et al.  A 13C NMR Study of Aqueous Dispersions of Reversed Lipid Phases , 2000 .

[45]  M. Almgren,et al.  Cubic Lipid−Water Phase Dispersed into Submicron Particles , 1996 .

[46]  K. Larsson.  Cubic lipid-water phases: structures and biomembrane aspects , 1989 .

[47]  N. Schore,et al.  Amino Acids, Peptides, Proteins, and Nucleic Acids , 2014 .

[48]  Jaymes R Beech,et al.  Targeted nanoparticles in imaging: paving the way for personalized medicine in the battle against cancer. , 2013, Integrative biology : quantitative biosciences from nano to macro.

[49]  M. Monduzzi,et al.  Physicochemical and rheological properties of a novel monoolein-based vesicle gel , 2013 .

[50]  Francesca Cuomo,et al.  Specific interactions between nucleolipid doped liposomes and DNA allow a more efficient polynucleotide condensation. , 2012, Journal of colloid and interface science.

[51]  Steven L. Murov,et al.  Handbook of photochemistry , 1973 .