Changes in monocyte functions of astronauts

As part of the systematic evaluation of the innate immune system for long duration missions, this study focused on the antimicrobial functions of monocytes in astronauts participating in spaceflight. The study included four space shuttle missions and 25 astronauts. Nine non-astronauts served as controls. Blood specimens were collected 10 days before launch, within 3h after landing, and again 3 days after landing. The number of monocytes did not differ significantly over the interval sampled in both the astronaut or control groups. However, following 5-11 days of spaceflight, the astronauts' monocytes exhibited reductions in ability to engulf Escherichia coli, elicit an oxidative burst, and degranulate. The phagocytic index was significantly reduced following spaceflight when compared to control values. This reduction in phagocytosis was accompanied by changes in the expression of two surface markers involved in phagocytosis, CD32 and CD64. Levels of cortisol, epinephrine, and norepinephrine after spaceflight did not increase over preflight values.

[1]  Felice Strollo,et al.  Chapter 4 Hormonal Changes in Humans During Spaceflight , 1999 .

[2]  A. Billingslea,et al.  Adherence-dependent calcium signaling in monocytes: induction of a CD14-high phenotype, stimulus-responsive subpopulation. , 1997, Journal of immunological methods.

[3]  G R Taylor,et al.  Immunological analyses of U.S. Space Shuttle crewmembers. , 1986, Aviation, space, and environmental medicine.

[4]  Daniel L. Feeback,et al.  Leukocyte subsets and neutrophil function after short‐term spaceflight , 1999, Journal of leukocyte biology.

[5]  Daniel L. Feeback,et al.  Decreased non-MHC-restricted (CD56+) killer cell cytotoxicity after spaceflight. , 2001, Journal of applied physiology.

[6]  A. Gewirtz,et al.  Neutrophil degranulation and phospholipase D activation are enhanced if the Na+/H+ antiport is blocked , 1998, Journal of leukocyte biology.

[7]  L S Neale,et al.  Alteration in human mononuclear leucocytes following space flight. , 1992, Immunology.

[8]  C S Leach,et al.  Endocrine responses in long-duration manned space flight. , 1975, Acta astronautica.

[9]  G Sonnenfeld,et al.  Changes in the immune system during and after spaceflight. , 1997, Advances in space biology and medicine.

[10]  G R Taylor,et al.  Effects of spaceflight on levels and activity of immune cells. , 1990, Aviation, space, and environmental medicine.

[11]  A. Schreiber,et al.  Molecular dissection of Fcγ receptor-mediated phagocytosis , 1995 .

[12]  Daniel L. Feeback,et al.  Immune responses and latent herpesvirus reactivation in spaceflight. , 2001, Aviation, space, and environmental medicine.

[13]  P. Newburger,et al.  Measurement of superoxide release in the phagovacuoles of immune complex-stimulated human neutrophils. , 1990, Journal of immunological methods.

[14]  I V Konstantinova,et al.  Immune changes during long‐duration missions , 1993, Journal of leukocyte biology.

[15]  T P Stein,et al.  Excretion of IL-6 by astronauts during spaceflight. , 1994, The American journal of physiology.

[16]  D. Pierson,et al.  Elevated Stress Hormone Levels Relate to Epstein-Barr Virus Reactivation in Astronauts , 2001, Psychosomatic medicine.

[17]  A. Gewirtz,et al.  A cytosolic calcium transient is not necessary for degranulation or oxidative burst in immune complex‐stimulated neutrophils , 1997, Journal of leukocyte biology.

[18]  D. Mirkin.  Space Physiology and Medicine , 1990 .

[19]  A. Greenough,et al.  Differential expression of Fc receptors for IgG by monocytes and granulocytes from neonates and adults , 1996, Clinical and experimental immunology.

[20]  G R Taylor,et al.  In vivo testing confirms a blunting of the human cell‐mediated immune mechanism during space flight , 1992, Journal of leukocyte biology.

[21]  I V Konstantinova.  Immune resistance of man in space flights. , 1991, Acta astronautica.

[22]  A. Levinson,et al.  Human Fc gamma RII, in the absence of other Fc gamma receptors, mediates a phagocytic signal. , 1991, The Journal of clinical investigation.

[23]  Victoria A Castro,et al.  Changes in neutrophil functions in astronauts , 2004, Brain, Behavior, and Immunity.

[24]  D. Pierson,et al.  Incidence of Epstein-Barr virus in astronaut saliva during spaceflight. , 1999, Aviation, space, and environmental medicine.

[25]  C S Leach,et al.  Medical results from STS 1-4: analysis of body fluids. , 1983, Aviation, space, and environmental medicine.

[26]  D. Pierson,et al.  Reactivation and shedding of cytomegalovirus in astronauts during spaceflight. , 2000, The Journal of infectious diseases.

[27]  P. Brandtzaeg,et al.  CD89: the human myeloid IgA Fc receptor. , 2001, Archivum immunologiae et therapiae experimentalis.

[28]  G. Sonnenfeld,et al.  Effect of microgravity modeling on interferon and interleukin responses in the rat. , 1991, Journal of interferon research.

[29]  R. Johnston.  Current concepts: immunology. Monocytes and macrophages. , 1988, The New England journal of medicine.

[30]  C. Tipton,et al.  Neuroendocrine and immune system responses with spaceflights. , 1996, Medicine and science in sports and exercise.