IMMUNOHISTOCHEMICAL ANALYSIS FOR CD21, CD35, CALDESMON AND S100 PROTEIN ON DENDRITIC CELLS TYPES IN ORAL LYMPHOMAS

Objective: Follicular dendritic cells (FDCs) and interdigitating dendritic cells (IDCs) are dendritic cells found in lymphoid follicles, reactive follicles and in lymphomas. The goal of this study was to evaluate the presence and distribution of FDCs and IDCs in oral lymphomas. Material and Methods: Immunohistochemistry reactions were applied to 50 oral lymphomas using the antibodies anti-CD21, anti-CD35 and anti-caldesmon to FDCs, and anti-S100 protein to IDCs. Caldesmon+/FDCs and S100+/IDCs were quantified in Imagelab® software. Results: FDCs revealed by CD21 and CD35 were positively stained in two cases of diffuse large B-cell lymphoma, one MALT lymphoma, and in one case of mantle cell lymphoma. FDCs were immunopositive to caldesmon in all cases, as well as IDCs to S100 protein. Burkitt lymphoma presented a lower amount of caldesmon+/FDCs and S100+/IDCs than diffuse large B-cell lymphoma and plasmablastic lymphoma of the oral mucosa type. Conclusions: The microenvironment determined by neoplastic lymphoid cells in oral lymphomas is responsible by the development and expression of dendritic cells types.

[1]  A. López-Guillermo,et al.  Diffuse Large B-cell Lymphomas With Plasmablastic Differentiation Represent a Heterogeneous Group of Disease Entities , 2004, The American journal of surgical pathology.

[2]  Hitoshi Suzuki,et al.  Immunohistochemical Recognition of Human Follicular Dendritic Cells (FDCs) in Routinely Processed Paraffin Sections , 2002, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[3]  D. Hossfeld.  E.S. Jaffe, N.L. Harris, H. Stein, J.W. Vardiman (eds). World Health Organization Classification of Tumours: Pathology and Genetics of Tumours of Haematopoietic and Lymphoid Tissues , 2002 .

[4]  P. Isaacson,et al.  Follicular dendritic cells in reactive and neoplastic lymphoid tissues: a reevaluation of staining patterns of CD21, CD23, and CD35 antibodies in paraffin sections after wet heat-induced epitope retrieval. , 2001 .

[5]  P. Isaacson,et al.  Follicular Dendritic Cells in Reactive and Neoplastic Lymphoid Tissues: A Reevaluation of Staining Patterns of CD21, CD23, and CD35 Antibodies in Paraffin Sections After Wet Heat-Induced Epitope Retrieval , 2001, Applied immunohistochemistry & molecular morphology : AIMM.

[6]  T. Takahashi,et al.  Differential expression of Ca(2+)-binding proteins on follicular dendritic cells in non-neoplastic and neoplastic lymphoid follicles. , 1999, The American journal of pathology.

[7]  P. Heerde,et al.  Follicular dendritic cell sarcoma and interdigitating reticulum cell sarcoma: A review , 1998, American journal of hematology.

[8]  D. Dorfman,et al.  Characteristic proliferations of reticular and dendritic cells in angioimmunoblastic lymphoma. , 1998, The American journal of surgical pathology.

[9]  M. Yamakawa,et al.  The lymphocyte-dendritic cell system. , 1998, Histology and histopathology.

[10]  G. Delsol,et al.  CNA.42, a new monoclonal antibody directed against a fixative-resistant antigen of follicular dendritic reticulum cells. , 1997, The American journal of pathology.

[11]  G. F. Burton,et al.  Follicular dendritic cells and presentation of antigen and costimulatory signals to B cells , 1997, Immunological reviews.

[12]  M. Yamakawa,et al.  Morphology, function and pathology of follicular dendritic cells , 1996, Pathology international.

[13]  A. Baur,et al.  Follicular dendritic cells in bone marrow lymphoproliferative diseases: an immunohistochemical study including a new paraffin‐resistant monoclonal antibody, DR53 , 1996, Histopathology.

[14]  M. Shinzato,et al.  Differentiation of Langerhans cells from interdigitating cells using CD1a and S-100 protein antibodies. , 1995, Biotechnic & histochemistry : official publication of the Biological Stain Commission.

[15]  G. Srivastava,et al.  Usefulness of follicular dendritic cell pattern in classification of peripheral T‐cell lymphomas , 1993, Histopathology.

[16]  M. Narabayashi,et al.  Follicular dendritic cells in malignant lymphomas--distribution, phenotypes & ultrastructures. , 1993, Advances in experimental medicine and biology.

[17]  J. Tew.  Follicular dendritic cells and dendritic cell nomenclature. , 1993, Advances in experimental medicine and biology.

[18]  A. Carbone,et al.  The nonlymphoid microenvironment of reactive follicles and lymphomas of follicular origin as defined by immunohistology on paraffin-embedded tissues. , 1993, Human pathology.

[19]  R. Steinman,et al.  The dendritic cell system and its role in immunogenicity. , 1991, Annual review of immunology.

[20]  K. Oka,et al.  DRC antigen expression in B-cell lymphomas. , 1988, American journal of clinical pathology.

[21]  R. Manconi,et al.  S‐100 protein, fibronectin, and laminin immunostaining in lymphomas of follicular center cell origin , 1986, Cancer.

[22]  J. Brooks,et al.  Leu M1 and S100 in Hodgkin's disease and non-Hodgkin's lymphomas. , 1986, American journal of clinical pathology.

[23]  E. Thorsby,et al.  Relative efficiency of human Langerhans' cells and blood derived dendritic cells as antigen-presenting cells. , 1985, Acta dermato-venereologica.

[24]  R. Warnke,et al.  Histiocytic malignancies: Morphologic, immunologic, and enzymatic heterogeneity , 1984, The American journal of surgical pathology.

[25]  H. Stein,et al.  Human dendritic reticulum cells of lymphoid follicles: Their antigenic profile and their identification as multinucleated giant cells , 1983, Virchows Archiv. B, Cell pathology including molecular pathology.