Influence of temperature on the sieving effect of different polymer matrices in capillary SDS gel electrophoresis of proteins

Two different sieving polymers, branched (dextran) and linear (poly(ethylene oxide)), were used in high-performance capillary gel electrophoresis to study the effect of temperature on the separation of SDS-protein complexes in the molecular mass range of 14 000-97 400 Da. Migration properties and band broadening of the proteins were studied as a function of column temperature ranging from 20 and 50 o C. Equations have been derived that relate the effect of temperature on the mobility of the solute

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