Increase of Junctional and Background 16S (Tailed, Asymmetric) Acetylcholinesterase During Postnatal Maturation of Rat and Mouse Sternocleidomastoid Muscle

Abstract: Acetylcholinesterase (AChE) is found both in motor end‐plate (MEP)‐free and MEP‐rich regions of rat or mouse muscle. We studied the developmental aspects of the localization of asymmetric 16S AChE in both regions of the sternocleidomastoid muscle, which has a well‐defined zone of motor innervation. In the rat, the proportion of 16S AChE to total AChE increases in the MEP‐rich region, and becomes significantly higher than in the MEP‐free regions between the first and the second weeks after birth. In the mouse, at birth, the MEP‐rich region already has a higher relative content in 16S AChE than the MEP‐free regions. Total 16S AChE amounts increase during postnatal development, not only in the MEP‐rich region but also in the MEP‐free regions. Thus, 16S AChE is not eliminated from MEP‐free regions during muscle maturation and growth. Two distinct pools of 16S AChE are distinguished in the muscles, both of which increase during postnatal development: junctional and background 16S AChE.

[1]  S. Schuetze,et al.  A post‐natal decrease in acetylcholine channel open time at rat end‐plates. , 1980, The Journal of physiology.

[2]  F. Rieger,et al.  Spontaneous contractile activity and the presence of the 16 S form of acetylcholinesterase in rat muscle cells in culture: reversible suppressive action of tetrodotoxin. , 1980, Developmental biology.

[3]  L. Rubin,et al.  Regulation of acetylcholinesterase appearance at neuromuscular junctions in vitro , 1980, Nature.

[4]  B. Festoff,et al.  CELLULAR DISTRIBUTION OF 16S ACETYLCHOLINESTERASE , 1979, Journal of neurochemistry.

[5]  M. Fardeau,et al.  Distribution of acetylcholinesterase molecular forms in neural and non‐neural sections of human muscle , 1979, FEBS letters.

[6]  B. Sakmann,et al.  Change in synaptic channel gating during neuromuscular development , 1978, Nature.

[7]  F. Rieger,et al.  THE MOTOR END‐PLATE SPECIFIC FORM OF ACETYLCHOLINESTERASE: APPEARANCE DURING EMBRYOGENESIS AND RE‐INNERVATION OF RAT MUSCLE , 1976, Journal of neurochemistry.

[8]  F. Rieger,et al.  SOLUBILIZATION AND PHYSICOCHEMICAL CHARACTERIZATION OF RAT BRAIN ACETYLCHOLINESTERASE: DEVELOPMENT AND MATURATION OF ITS MOLECULAR FORMS , 1976, Journal of neurochemistry.

[9]  Y. Dudai,et al.  Molecular structures of acetylcholinesterase from electric organ tissue of the electric eel. , 1973, Proceedings of the National Academy of Sciences of the United States of America.

[10]  F. Rieger,et al.  Observation par microscopie électronique des formes allongées et globulaires de l'acétylcholinestérase de gymnote (Electrophorus electricus) , 1973 .

[11]  S. Mori,et al.  Innervation and the regulation of acetylcholinesterase activity during the development of normal and dystrophic chick muscle. , 1970, The Journal of experimental zoology.

[12]  F. Rieger,et al.  L'acétylcholinestérase des organes électriques de Poissons (torpille et gymnote); complexes membranaires , 1969 .

[13]  D. Grob,et al.  CHOLINESTERASE ACTIVITY OF THE MOTOR ENDPLATE IN ISOLATED MUSCLE MEMBRANE , 1968, Journal of neurochemistry.

[14]  K. Larsson,et al.  HISTOCHEMICAL DIFFERENTIATION OF SKELETAL MUSCLE IN FOETAL AND NEWBORN MICE. , 1964, Journal of embryology and experimental morphology.

[15]  K. Courtney,et al.  A new and rapid colorimetric determination of acetylcholinesterase activity. , 1961, Biochemical pharmacology.

[16]  J. Zajicek,et al.  Quantitative Determination of Cholinesterase Activity in Individual End-plates of Normal and Denervated Gastrocnemius Muscle , 1958, Nature.

[17]  Oliver H. Lowry,et al.  Protein measurement with the Folin phenol reagent. , 1951, The Journal of biological chemistry.

[18]  G B KOELLE,et al.  A Histochemical Method for Localizing Cholinesterase Activity.* , 1949, Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine.

[19]  J. Massoulie,et al.  The molecular forms of cholinesterase and acetylcholinesterase in vertebrates. , 1982, Annual review of neuroscience.

[20]  F. Rieger,et al.  Biochemical stability of the AChE molecular forms after cytochemical staining: postnatal focalization of the 16S AChE in rat muscle. , 1981, Developmental neuroscience.

[21]  Z. Hall,et al.  Multiple forms of acetylcholinesterase and their distribution in endplate and non-endplate regions of rat diaphragm muscle. , 1973, Journal of neurobiology.