Highly efficient immobilization of glycosylated enzymes into polyurethane foams.

Glycosylated enzymes, including aminoacylase from Aspergillus melleus, chloroperoxidase from Caldariomyces fumago, and phytase from Aspergillus ficuum, were covalently immobilized into polyurethane foams with very high enzyme loadings of up to 0.2 g protein per gram dry foam. The immobilization efficiency (retained activity) ranged from 100% at a low loading to 60% at high loadings. In contrast to many other immobilization methods no leaching of the enzyme from the support took place under the reaction conditions. In short, a universal method for the immobilization of enzymes from fungal sources was developed, affording a highly active, stable, and reusable biocatalyst.

[1]  R. Sheldon,et al.  Chloroperoxidase-catalyzed enantioselective oxidations in hydrophobic organic media. , 2001, Biotechnology and bioengineering.

[2]  A. Russell,et al.  Covalent binding of a nerve agent hydrolyzing enzyme within polyurethane foams , 2000, Biotechnology and bioengineering.

[3]  R. Sheldon,et al.  The rational design of semisynthetic peroxidases. , 2000, Biotechnology and bioengineering.

[4]  A. Basavapathruni,et al.  Chiral synthons via chloroperoxidase catalysis , 1998 .

[5]  C. R. Johnson,et al.  Organic synthesis using biocatalytically generated intermediates. , 1998, Current opinion in chemical biology.

[6]  R. Sheldon,et al.  Selective oxidations catalyzed by peroxidases , 1997 .

[7]  J. Grimsley,et al.  Dramatically stabilized phosphotriesterase-polymers for nerve agent degradation. , 1997, Biotechnology and bioengineering.

[8]  R. Sheldon,et al.  Chloroperoxidase catalyzed oxidations in t-butyl alcohol/water mixtures , 1997 .

[9]  Clemens Broger,et al.  Crystal structure of phytase from Aspergillus ficuum at 2.5 Å resolution , 1997, Nature Structural Biology.

[10]  E. Katchalski‐Katzir,et al.  Immobilized enzymes--learning from past successes and failures. , 1993, Trends in biotechnology.

[11]  C. Wandrey,et al.  Operational stability of enzymes. Acylase-catalyzed resolution of N-acetyl amino acids to enantiomerically pure L-amino acids. , 1992, Annals of the New York Academy of Sciences.

[12]  C. Wandrey,et al.  Operational Stability of Enzymes , 1992 .

[13]  L. Thim,et al.  A model for interfacial activation in lipases from the structure of a fungal lipase-inhibitor complex , 1991, Nature.

[14]  K. Storey,et al.  Immobilization of amyloglucosidase using two forms of polyurethane polymer , 1990, Applied biochemistry and biotechnology.

[15]  K. Storey,et al.  One-step conversion of cellulose to fructose using coimmobilized cellulase, β-glucosidase, and glucose isomerase , 1990 .

[16]  K. Storey,et al.  Enhanced glucose production from cellulose using coimmobilized cellulase and β-glucosidase , 1989 .

[17]  K. Storey,et al.  Immobilization of cellulase using polyurethane foam , 1988 .

[18]  N. Nishio,et al.  Production of a Thermostable Lipase by Humicola lanuginosa Grown on Sorbitol-Corn Steep Liquor Medium , 1987 .

[19]  L. Hager,et al.  Post-translational modifications of chloroperoxidase from Caldariomyces fumago. , 1987, Archives of biochemistry and biophysics.

[20]  B. Svensson,et al.  Structural studies on the O-glycosidically linked carbohydrate chains of glucoamylase G1 from Aspergillus niger. , 1984, European journal of biochemistry.

[21]  H. Löffler,et al.  Aminoacylase from Aspergillus oryzae. Comparison with the Pig Kidney Enzyme , 1980, Zeitschrift fur Naturforschung. Section C, Biosciences.

[22]  T. Yoshimoto,et al.  Purification and comparison of two types of beta -galactosidases from Aspergillus oryzae. , 1980 .

[23]  D. Morris,et al.  Chloroperoxidase. I. Isolation and properties of the crystalline glycoprotein. , 1966, The Journal of biological chemistry.

[24]  F. V. D. Velde,et al.  Enantioselective sulfoxidation mediated by vanadium-incorporated phytase: a hydrolase acting as a peroxidase , 1998 .

[25]  R. Sheldon,et al.  Stabilisation of lipases for activity in ammoniolysis , 1998 .

[26]  R. Sheldon,et al.  A SIMPLE PURIFICATION METHOD FOR CHLOROPEROXIDASE AND ITS USE IN ORGANIC MEDIA , 1994 .

[27]  B. McCleary,et al.  Purification of β-d-glucosidase from Aspergillus niger , 1988 .