Isolation and Characterization of the Human CP 49 Gene Promoter

METHODS. The DNA sequence upstream of the human CP49 coding region was subcloned as a set of 5 and 3 deletion series. The constructs were transfected into lens (N/N1003A) and nonlens (NIH3T3) cell lines and chicken primary lens cultures, to test for promoter activity and specificity. To further test the specificity, a portion of the 5 flanking DNA sequence was used to drive transgene expression in mice. The flanking DNA sequence was analyzed for potential transcription factor– binding sites.

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