Phenol biodegradation by Pseudomonas putida DSM 548 in a batch reactor.

Phenol biodegradation in a batch reactor using a pure culture of Pseudomonas putida DSM 548 was studied. The purpose of the experiments was to determine the kinetics of biodegradation by measuring biomass growth rates and phenol concentration as a function of time in a batch reactor. The Haldane equation µ=µ(m)S/((K(s)+S+S(2))/K(i)) adequately describes cell growth with kinetic constants µ(m)=0.436h(-1), K(s)=6.19mgl(-1), K(i)=54.1mgl(-1). These values are in the range of those published in literature for pure or mixed cultures degrading phenol.

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