Recent advances in DNA sensors.

The concept of DNA biosensors is sustained by the need for rapid and highly sensitive analytical tools for genetic detection. Their implementation is based on three key steps: (i) immobilization of single-stranded oligonucleotide probes onto a substrate; (ii) hybridization and (iii) reading. These steps involve complementary knowledge in various disciplinary fields such as surface physics and chemistry, molecular electrochemistry, micro-technologies, optics, electronics and biochemistry. We present here, in a non-exhaustive way, the recent advances in the two steps of immobilization and detection that rely upon increasing integration of the number of reading points or/and of the reading strategy.

[1]  Pedro Estrela,et al.  Label-free electrical detection of DNA hybridization for the example of influenza virus gene sequences. , 2008, Analytical biochemistry.

[2]  M. Ozsoz,et al.  Design of electrochemical biosensor systems for the detection of specific DNA sequences in PCR-amplified nucleic acids related to the catechol-O-methyltransferase Val108/158Met polymorphism based on intrinsic guanine signal. , 2008, Analytical chemistry.

[3]  Miroslav Fojta,et al.  Magnetic beads as versatile tools for electrochemical DNA and protein biosensing. , 2007, Talanta.

[4]  A. Erdem,et al.  Nanomaterial-based electrochemical DNA sensing strategies. , 2007, Talanta.

[5]  Carole Chaix,et al.  Cylinder-shaped conducting polypyrrole for labelless electrochemical multidetection of DNA. , 2007, Biosensors & bioelectronics.

[6]  P. Kara,et al.  Electrochemical probe DNA design in PCR amplicon sequence for the optimum detection of microbiological diseases. , 2007, Bioelectrochemistry.

[7]  Séamus P J Higson,et al.  Advances in the application of scanning electrochemical microscopy to bioanalytical systems. , 2007, Biosensors & bioelectronics.

[8]  Filip Braet,et al.  Carbon nanotubes for biological and biomedical applications , 2007 .

[9]  Chad A Mirkin,et al.  Nonenzymatic detection of bacterial genomic DNA using the bio bar code assay. , 2007, Analytical chemistry.

[10]  A. Belcher,et al.  Label-free and high-resolution protein/DNA nanoarray analysis using Kelvin probe force microscopy. , 2007, Nature nanotechnology.

[11]  Laura K. Povlich,et al.  Label‐Free and Self‐Signal Amplifying Molecular DNA Sensors Based on Bioconjugated Polyelectrolytes , 2007 .

[12]  Raj Mutharasan,et al.  PEMC-based method of measuring DNA hybridization at femtomolar concentration directly in human serum and in the presence of copious noncomplementary strands. , 2007, Analytical chemistry.

[13]  Itamar Willner,et al.  Amplified electrochemical detection of DNA through the aggregation of Au nanoparticles on electrodes and the incorporation of methylene blue into the DNA-crosslinked structure. , 2007, Chemical communications.

[14]  Wolfgang Knoll,et al.  Graded‐Bandgap Quantum‐ Dot‐Modified Nanotubes: A Sensitive Biosensor for Enhanced Detection of DNA Hybridization , 2007 .

[15]  Abraham J. Qavi,et al.  Nanoparticle-enhanced diffraction gratings for ultrasensitive surface plasmon biosensing. , 2007, Analytical chemistry.

[16]  Chengxiao Zhang,et al.  Ultrasensitive electrogenerated chemiluminescence detection of DNA hybridization using carbon-nanotubes loaded with tris(2,2'-bipyridyl) ruthenium derivative tags. , 2007, Talanta.

[17]  S. Jockusch,et al.  Inorganic-organic hybrid luminescent binary probe for DNA detection based on spin-forbidden resonance energy transfer. , 2007, Journal of the American Chemical Society.

[18]  T. Livache,et al.  Fabrication of Oligonucleotide Chips by Using Parallel Cantilever‐Based Electrochemical Deposition in Picoliter Volumes , 2007 .

[19]  Ronen Polsky,et al.  Selective immobilization of DNA and antibody probes on electrode arrays: simultaneous electrochemical detection of DNA and protein on a single platform. , 2007, Langmuir : the ACS journal of surfaces and colloids.

[20]  Katherine J Odenthal,et al.  An introduction to electrochemical DNA biosensors. , 2007, The Analyst.

[21]  B. Limoges,et al.  Evaluation of the analytical performances of avidin-modified carbon sensors based on a mediated horseradish peroxidase enzyme label and their application to the amperometric detection of nucleic acids. , 2007, Biosensors & bioelectronics.

[22]  A. Star,et al.  Carbon Nanotube Field‐Effect‐Transistor‐Based Biosensors , 2007 .

[23]  T. Su,et al.  DNA nanoswitch as a biosensor. , 2007, Analytical chemistry.

[24]  Kang Wang,et al.  Exploring the motional dynamics of end-grafted DNA oligonucleotides by in situ electrochemical atomic force microscopy. , 2007, The journal of physical chemistry. B.

[25]  R. Miranda-Castro,et al.  Hairpin-DNA probe for enzyme-amplified electrochemical detection of Legionella pneumophila. , 2007, Analytical chemistry.

[26]  E. Tamiya,et al.  Excitation of localized surface plasmon resonance using a core–shell structured nanoparticle layer substrate and its application for label-free detection of biomolecular interactions , 2007 .

[27]  M. Mirkin,et al.  Electrochemical detection of lateral charge transport in metal complex-DNA monolayers synthesized on Si(1 1 1) electrodes , 2007 .

[28]  S. Jockusch,et al.  Fluorescent hybridization probes for sensitive and selective DNA and RNA detection. , 2007, Accounts of chemical research.

[29]  S. Hernández,et al.  In situ DNA amplification with magnetic primers for the electrochemical detection of food pathogens. , 2007, Biosensors & bioelectronics.

[30]  Muhammad J A Shiddiky,et al.  Trace analysis of DNA: preconcentration, separation, and electrochemical detection in microchip electrophoresis using Au nanoparticles. , 2007, Analytical chemistry.

[31]  Zhiqiang Gao,et al.  Silicon nanowire arrays for label-free detection of DNA. , 2007, Analytical chemistry.

[32]  Nicole Jaffrezic-Renault,et al.  Biosensors and Bio-Bar Code Assays Based on Biofunctionalized Magnetic Microbeads , 2007, Sensors (Basel, Switzerland).

[33]  Tomoji Kawai,et al.  A review of DNA functionalized/grafted carbon nanotubes and their characterization , 2007 .

[34]  R. Thewes,et al.  A Fully Electronic Label-Free DNA Sensor Chip , 2007, IEEE Sensors Journal.

[35]  C. M. Elliott,et al.  Indirect electrochemical sensing of DNA hybridization based on the catalytic oxidation of cobalt (II). , 2007, Journal of the American Chemical Society.

[36]  Akira Fujishima,et al.  Simultaneous detection of purine and pyrimidine at highly boron-doped diamond electrodes by using liquid chromatography. , 2007, Talanta.

[37]  Frank Davis,et al.  Single gene differentiation by DNA-modified carbon electrodes using an AC impedimetric approach. , 2007, Analytical chemistry.

[38]  T. Livache,et al.  Temperature effects on DNA chip experiments from surface plasmon resonance imaging: isotherms and melting curves. , 2007, Biophysical journal.

[39]  Justin A. Ionita,et al.  Selective release of DNA from the surface of indium-tin oxide thin electrode films using thiol-disulfide exchange chemistry. , 2007, Analytical chemistry.

[40]  G. Flechsig,et al.  Electrochemical detection of DNA hybridization by means of osmium tetroxide complexes and protective oligonucleotides. , 2007, Analytical chemistry.

[41]  Tomoji Kawai,et al.  Peptide-nucleic acid-modified ion-sensitive field-effect transistor-based biosensor for direct detection of DNA hybridization. , 2007, Analytical chemistry.

[42]  I. Willner,et al.  Biomolecule-nanoparticle hybrid systems for bioelectronic applications. , 2007, Bioelectrochemistry.

[43]  C. E. Mills,et al.  Modified rare earth semiconductor oxide as a new nucleotide probe. , 2006, The journal of physical chemistry. B.

[44]  James R Heath,et al.  Quantitative real-time measurements of DNA hybridization with alkylated nonoxidized silicon nanowires in electrolyte solution. , 2006, Journal of the American Chemical Society.

[45]  W. Grange,et al.  Rapid and label-free nanomechanical detection of biomarker transcripts in human RNA , 2006, Nature nanotechnology.

[46]  S. Cosnier,et al.  A polypyrrole cDNA electrode for the amperometric detection of the West Nile Virus , 2006 .

[47]  Hiroshi Kawarada,et al.  Label-free DNA sensors using ultrasensitive diamond field-effect transistors in solution. , 2006, Physical review. E, Statistical, nonlinear, and soft matter physics.

[48]  Luigi Raffo,et al.  Fully electronic DNA hybridization detection by a standard CMOS biochip , 2006 .

[49]  S. Cosnier,et al.  Electroenzymatic polypyrrole-intercalator sensor for the determination of West Nile virus cDNA. , 2006, Analytical chemistry.

[50]  Itamar Willner,et al.  An autonomous fueled machine that replicates catalytic nucleic acid templates for the amplified optical analysis of DNA , 2006, Nature Protocols.

[51]  B. Limoges,et al.  Subfemtomolar electrochemical detection of target DNA by catalytic enlargement of the hybridized gold nanoparticle labels. , 2006, The Analyst.

[52]  R. Hintsche,et al.  Automated detection and quantitation of bacterial RNA by using electrical microarrays. , 2006, Analytical chemistry.

[53]  Steven A. Soper,et al.  Purification and preconcentration of genomic DNA from whole cell lysates using photoactivated polycarbonate (PPC) microfluidic chips , 2006, Nucleic acids research.

[54]  Joseph Wang,et al.  Electrochemical biosensors: towards point-of-care cancer diagnostics. , 2006, Biosensors & bioelectronics.

[55]  Andreas Offenhäusser,et al.  Detection of DNA hybridization by a field‐effect transistor with covalently attached catcher molecules , 2006 .

[56]  D. Leech,et al.  Redox polymer and probe DNA tethered to gold electrodes for enzyme-amplified amperometric detection of DNA hybridization. , 2006, Analytical chemistry.

[57]  Kurt V Gothelf,et al.  Femtomolar electrochemical detection of DNA targets using metal sulfide nanoparticles. , 2006, Journal of the American Chemical Society.

[58]  Taekjip Ha,et al.  Single molecule nanometronome. , 2006, Nano letters.

[59]  Zhiqiang Gao,et al.  Nanoparticles in biomolecular detection , 2006 .

[60]  H. Kondo,et al.  Imaging of DNA microarray with scanning electrochemical microscopy , 2006 .

[61]  Sabine Szunerits,et al.  Imaging of DNA hybridization on microscopic polypyrrole patterns using scanning electrochemical microscopy (SECM): the HRP bio-catalyzed oxidation of 4-chloro-1-naphthol. , 2006, The Analyst.

[62]  Frédéric Ginot,et al.  Electrical detection of DNA hybridization based on enzymatic accumulation confined in nanodroplets. , 2005, Analytical chemistry.

[63]  Igor L. Medintz,et al.  Quantum dot bioconjugates for imaging, labelling and sensing , 2005, Nature materials.

[64]  Yongrae Roh,et al.  Development of an SH-SAW sensor for the detection of DNA hybridization , 2005 .

[65]  B. Piro,et al.  DNA electrochemical sensor based on conducting polymer: dependence of the "signal-on" detection on the probe sequence localization. , 2005, Analytical chemistry.

[66]  Sara Tombelli,et al.  Direct immobilisation of DNA probes for the development of affinity biosensors. , 2005, Bioelectrochemistry.

[67]  Joseph Wang.  Nanomaterial-based electrochemical biosensors. , 2005, The Analyst.

[68]  Thierry Livache,et al.  Micro‐Imprinting of Oligonucleotides and Oligonucleotide Gradients on Gold Surfaces: A New Approach Based on the Combination of Scanning Electrochemical Microscopy and Surface Plasmon Resonance Imaging (SECM/ SPR‐i) , 2005 .

[69]  S. Cosnier,et al.  New flavin and deazaflavin oligonucleotide conjugates for the amperometric detection of DNA hybridization. , 2004, Chemical communications.

[70]  Hafsa Korri-Youssoufi,et al.  Toward Bioelectronics: Specific DNA Recognition Based on an Oligonucleotide-Functionalized Polypyrrole , 1997 .