A root-specific bZIP transcription factor is responsive to water deficit stress in tepary bean (Phaseolus acutifolius) and common bean (P. vulgaris).

Root cDNA libraries were differentially screened to isolate water deficit-responsive transcripts in the relatively drought-resistant plant tepary bean (Phaseolus acutifolius). A novel root-specific, water deficit-responsive transcript was identified and predicted to encode a bZIP transcription factor. The orthologous form of this gene was isolated from the drought-sensitive P. vulgaris and the patterns of expression of these genes compared. These genes have predicted amino acid sequences in the bZIP domain that are 64% similar to a soybean bZIP protein. There were three amino acid differences between the P. acutifolius bZIP and the P. vulgaris gene product. Both species transcribed this gene in a root-specific and water deficit-responsive manner. The cell-specific pattern of expression for the gene was determined using in situ hybridization and immunolocalization. Two tissues in the root accumulated the protein: epidermis and phloem. The nuclear localization of this protein was determined by electron microscopy. The bZIP protein accumulated in the nuclei of both the epidermal cell and the vascular cell in response to water deficit stress in both species in a similar manner.

[1]  P. Miklas,et al.  Field performance of select tepary bean germplasm in the tropics , 1994 .

[2]  Y. Kamiya,et al.  REPRESSION OF SHOOT GROWTH, a bZIP Transcriptional Activator, Regulates Cell Elongation by Controlling the Level of Gibberellins , 2000, Plant Cell.

[3]  C. Lovatt,et al.  Relationship between Relative Water Content, Nitrogen Pools, and Growth of Phaseolus vulgaris L. and P. acutifolius A. Gray during Water Deficit , 1999 .

[4]  M. Schuler,et al.  Methods in Plant Molecular Biology , 1988 .

[5]  B. Weisshaar,et al.  Transactivation properties of parsley proline-rich bZIP transcription factors. , 2000, The Plant journal : for cell and molecular biology.

[6]  L. Frusciante,et al.  Common Bean (Phaseolus vulgaris L.) Mutants , 1993 .

[7]  G. Perdew,et al.  Regulation of Gene Expression , 2008, Goodman's Medical Cell Biology.

[8]  W. Terzaghi,et al.  DNA binding site preferences and transcriptional activation properties of the Arabidopsis transcription factor GBF1. , 1992, The EMBO journal.

[9]  Shree P. Singh,et al.  Comparison of Sources and Lines Selected for Drought Resistance in Common Bean. , 2002, Crop science.

[10]  E. Bray.  Molecular Responses to Water Deficit , 1993, Plant physiology.

[11]  D. Wilkinson.  In situ hybridization: a practical approach , 1998 .

[12]  T. MacRae,et al.  Transcription factors and their genes in higher plants functional domains, evolution and regulation. , 1999, European journal of biochemistry.

[13]  G. Xue,et al.  HvDRF1 is involved in abscisic acid-mediated gene regulation in barley and produces two forms of AP2 transcriptional activators, interacting preferably with a CT-rich element. , 2004, The Plant journal : for cell and molecular biology.

[14]  T. Heinekamp,et al.  BZI-1 specifically heterodimerises with the tobacco bZIP transcription factors BZI-2, BZI-3/TBZF and BZI-4, and is functionally involved in flower development. , 2002, The Plant journal : for cell and molecular biology.

[15]  M. Bor,et al.  Differential responses of lipid peroxidation and antioxidants in the leaves of drought-tolerant P. acutifolius Gray and drought-sensitive P. vulgaris L. subjected to polyethylene glycol mediated water stress , 2005 .

[16]  F. Parcy,et al.  bZIP transcription factors in Arabidopsis The bZIP Research Group (Marc Jakoby et al.) , 2002 .

[17]  International review of cytology. Vol. IV. , 1954 .

[18]  S. Mundree,et al.  Molecular characterization of XVT8, a stress-responsive gene from the resurrection plant Xerophyta viscosa Baker , 2001, Plant Growth Regulation.

[19]  V. Citovsky,et al.  Preservation of plant cell ultrastructure during immunolocalization of virus particles. , 1998, Journal of virological methods.

[20]  M. Norman,et al.  The Ecology of Tropical Food Crops: Common bean ( Phaseolus vulgaris ) , 1995 .

[21]  S. Yanagisawa.  Transcription factors in plants: Physiological functions and regulation of expression , 1998, Journal of Plant Research.

[22]  J. White,et al.  Relative Effect of Root and Shoot Genotypes on Yield of Common Bean under Drought Stress , 1989 .

[23]  M. Aluru,et al.  Differential expression of fatty acid synthase genes, Acl, Fat and Kas, in Capsicum fruit. , 2003, Journal of experimental botany.

[24]  E. Burnett,et al.  Regulation of gene expression during water deficit stress , 1999, Plant Growth Regulation.

[25]  J. Boyer.  Plant Productivity and Environment , 1982, Science.

[26]  B. Keller,et al.  In vitro binding of the tomato bZIP transcriptional activator VSF-1 to a regulatory element that controls xylem-specific gene expression. , 1996, The Plant journal : for cell and molecular biology.

[27]  D. Nebert.  Transcription factors and cancer: an overview. , 2002, Toxicology.

[28]  D. Garvin,et al.  Isozyme evidence supporting a single geographic origin for domesticated tepary bean , 1994 .

[29]  E. Bray.  Classification of genes differentially expressed during water-deficit stress in Arabidopsis thaliana: an analysis using microarray and differential expression data. , 2002, Annals of botany.

[30]  N. Suzuki,et al.  A maize DNA-binding factor with a bZIP motif is induced by low temperature , 1995, Molecular and General Genetics MGG.

[31]  C. Lamb,et al.  RF2a, a bZIP transcriptional activator of the phloem‐specific rice tungro bacilliform virus promoter, functions in vascular development , 1997, The EMBO journal.

[32]  P. Goldsworthy,et al.  The Physiology of tropical field crops , 1984 .

[33]  F. Parcy,et al.  bZIP transcription factors in Arabidopsis. , 2002, Trends in plant science.

[34]  K. Shinozaki,et al.  Arabidopsis basic leucine zipper transcription factors involved in an abscisic acid-dependent signal transduction pathway under drought and high-salinity conditions. , 2000, Proceedings of the National Academy of Sciences of the United States of America.

[35]  G. Bellemare,et al.  A proline-, threonine-, and glycine-rich protein down-regulated by drought is localized in the cell wall of xylem elements. , 1999, Plant physiology.

[36]  Z. Tabaeizadeh.  Drought-induced responses in plant cells. , 1998, International review of cytology.

[37]  G. English-loeb.  Plant drought stress and outbreaks of spider mites: a field test. , 1990 .

[38]  R. Quatrano,et al.  Characterization of Three Rice Basic/Leucine Zipper Factors, Including Two Inhibitors of EmBP-1 DNA Binding Activity* , 1996, The Journal of Biological Chemistry.

[39]  J. Kelly,et al.  Traits related to drought resistance in common bean , 1998, Euphytica.