Composite Combined with Mesenchymal Stem Cells Repair of Segmental Defects with Nano-hydroxyapatite / Collagen / PLA

The aim of the present study was to investigate and compare the capacity of fresh-frozen allogeneic bone, nano-hydroxyapatite/collagen/PLA (nHAC/PLA) scaffold, and nHAC/PLA scaffold loaded with bone marrow mesenchymal stem cells (BMSCs) in inducing bone formation. A 10mm segmental rabbit radial defect was surgically created. The animals were divided into four groups in which the defect was either left untreated, or filled with the abovementioned three grafts. The animals were euthanized at 2, 4, 6, 8, 12, and 18 weeks. Radiographic and histologic analyses were performed on the harvested tissue. We show that nHAC/PLA composite combined with mesenchymal stem cells could enhance and accelerate bone formation in segmental defects of rabbits. nHAC/PLA composite is an ideal bone graft; implanting nHAC/PLA composite combined with mesenchymal stem cells is a potential method for surgical treatment of bone defects. *Author to whom correspondence should be addressed. E-mail: cuifz@mail.tsinghua.edu.cn Figure 2 appears in color online: http://jbc.sagepub.com Journal of BIOACTIVE AND COMPATIBLE POLYMERS, Vol. 21—September 2006 373 0883-9115/06/05 373–12 $10.00/0 DOI: 10.1177/0883911506068554 © 2006 SAGE Publications © 2006 SAGE Publications. All rights reserved. Not for commercial use or unauthorized distribution. at PENNSYLVANIA STATE UNIV on April 10, 2008 http://jbc.sagepub.com Downloaded from

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