Dynamic Expression and Regulation of Urotensin I and Corticotropin-Releasing Hormone Receptors in Ovary of Olive Flounder Paralichthys olivaceus

Urotensin I (UI), a fish corticotropin-releasing hormone (CRH) like peptide, has been found throughout vertebrate species that has great effects on adaptive physiology comprising stress-related responses, and osmotic regulation by binding with CRH type I receptor (CRHR1) and CRH type II receptor (CRHR2) in fish. Dynamic expression and regulation of UI and CRH receptors in the olive flounder ovarian follicle were studied so as to make further efforts to understand the role of UI in the development of teleost ovary. The results showed that stage-specific change in UI mRNA levels in ovarian follicles of olive flounder. UI and CRHR1 mRNA levels were higher in stage III follicles (300∼500 μm diameter) compared to stage II (90∼300 μm diameter) and IV (500∼800 μm diameter) follicles, however, the levels of CRHR2 mRNA were decreased in line with the ovarian development from stage II to stage IV. A strong signal of UI protein was observed in the follicular cells and oocyte in stage III and IV follicles by immunohistochemistry. In vitro treatment of olive flounder ovarian follicles with human chorionic gonadotropin (hCG) showed that the mRNA expression of UI increased significantly at low concentration and decreased at high concentration at 6 h, but the CRHR1 and CRHR2 mRNA did not change obviously. In addition, the results of incubation with 17α, 20β-dihydroxy-4-oregnen-3-one (DHP) show that UI and CRHR1 mRNA expression were elevated with increasing concentrations at 9 h. All above results indicated that UI and CRH receptors may have a vital effect on olive flounder ovarian development.

[1]  A. Makrigiannakis,et al.  CRH Receptors in Human Reproduction. , 2018, Current molecular pharmacology.

[2]  Miyuki Mekuchi,et al.  Greater amberjack Fsh, Lh, and their receptors: Plasma and mRNA profiles during ovarian development. , 2016, General and comparative endocrinology.

[3]  A. Fostier,et al.  Ovarian development and related changes in steroid hormones in female wild common carp (Cyprinus carpio carpio), from the south-eastern Caspian Sea. , 2014, Journal of animal physiology and animal nutrition.

[4]  Nanami Mizusawa,et al.  Cloning of corticotropin-releasing hormone (CRH) precursor cDNA and immunohistochemical detection of CRH peptide in the brain of the Japanese eel, paying special attention to gonadotropin-releasing hormone , 2014, Cell and Tissue Research.

[5]  H. Wen,et al.  DNA methylation status of cyp17-II gene correlated with its expression pattern and reproductive endocrinology during ovarian development stages of Japanese flounder (Paralichthys olivaceus). , 2013, Gene.

[6]  I. Ozerol,et al.  Effect of ovarian stimulation with recombinant follicle-stimulating hormone, gonadotropin-releasing hormone agonist and antagonists, on follicular fluid stem cell factor and serum urocortin 1 levels on the day of oocyte retrieval , 2013, Archives of Gynecology and Obstetrics.

[7]  B. Levavi-Sivan,et al.  Steroidogenic response of carp ovaries to piscine FSH and LH depends on the reproductive phase. , 2012, General and comparative endocrinology.

[8]  Guixing Wang,et al.  Genetic verification of doubled haploid Japanese flounder, Paralichthys olivaceus by genotyping telomeric microsatellite loci , 2012 .

[9]  U. Jeschke,et al.  Expression of CRH, CRH-related peptide and CRH receptor in the ovary and potential CRH signalling pathways. , 2011, Journal of reproductive immunology.

[10]  U. Rai,et al.  Immunomodulatory role of urotensins in teleost Channa punctatus. , 2011, General and comparative endocrinology.

[11]  C. Bethea,et al.  Ovarian steroid regulation of the midbrain corticotropin releasing factor and urocortin systems in macaques , 2010, Neuroscience.

[12]  S. Bernaudo,et al.  Regulation of membrane progestin receptors in the zebrafish ovary by gonadotropin, activin, TGF-β and BMP-15 , 2009, Molecular and Cellular Endocrinology.

[13]  A. Makrigiannakis,et al.  CRH-like peptides in human reproduction. , 2009, Current medicinal chemistry.

[14]  D. Lovejoy.  Structural evolution of urotensin-I: reflections of life before corticotropin releasing factor. , 2009, General and comparative endocrinology.

[15]  M. Hollenberg,et al.  Urotensin I-CRF-Urocortins: a mermaid's tail. , 2009, General and comparative endocrinology.

[16]  Z. She,et al.  Structural ensemble dynamics based closure model for wall-bounded turbulent flow , 2009 .

[17]  G. Macchi,et al.  Gonadal Sex Differentiation in Cultured Juvenile Flounder, Paralichthys orbignyanus (Valenciennes, 1839) , 2009 .

[18]  R. Fernald,et al.  Sequences, expression patterns and regulation of the corticotropin-releasing factor system in a teleost. , 2008, General and comparative endocrinology.

[19]  T. Kitahashi,et al.  Seasonal changes in CRF-I and urotensin I transcript levels in masu salmon: correlation with cortisol secretion during spawning. , 2008, General and comparative endocrinology.

[20]  Jing Xu,et al.  Expression and role of the corticotropin-releasing hormone/urocortin-receptor-binding protein system in the primate corpus luteum during the menstrual cycle. , 2007, Endocrinology.

[21]  C. Mccrohan,et al.  Seasonal changes in peptide, receptor and ion channel mRNA expression in the caudal neurosecretory system of the European flounder (Platichthys flesus). , 2007, General and comparative endocrinology.

[22]  S. Mousa,et al.  Involvement of corticotropin-releasing factor and adrenocorticotropic hormone in the ovarian maturation, seawater acclimation, and induced spawning of Liza ramada. , 2006, General and comparative endocrinology.

[23]  R. Stouffer,et al.  Dynamic expression and regulation of the corticotropin-releasing hormone/urocortin-receptor-binding protein system in the primate ovary during the menstrual cycle. , 2006, The Journal of clinical endocrinology and metabolism.

[24]  N. Bernier.  The corticotropin-releasing factor system as a mediator of the appetite-suppressing effects of stress in fish. , 2006, General and comparative endocrinology.

[25]  P. M. Craig,et al.  Differential increase in forebrain and caudal neurosecretory system corticotropin-releasing factor and urotensin I gene expression associated with seawater transfer in rainbow trout. , 2005, Endocrinology.

[26]  C. Mccrohan,et al.  Coexpression of corticotropin-releasing hormone and urotensin i precursor genes in the caudal neurosecretory system of the euryhaline flounder (Platichthys flesus): a possible shared role in peripheral regulation. , 2004, Endocrinology.

[27]  F. Petraglia,et al.  Urocortins in human reproduction , 2004, Peptides.

[28]  H. Sasano,et al.  Localization and physiological roles of urocortin , 2004, Peptides.

[29]  G. Elgar,et al.  Isolation and Characterisation of the Corticotropin Releasing Factor Receptor 1 (CRFR1) Gene in a Teleost Fish, Fugu rubripes , 2003, DNA sequence : the journal of DNA sequencing and mapping.

[30]  Thomas D. Schmittgen,et al.  Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. , 2001, Methods.

[31]  M. Darlison,et al.  Cloning and functional pharmacology of two corticotropin-releasing factor receptors from a teleost fish. , 2001, European journal of pharmacology.

[32]  M. Hongo,et al.  Urocortin and corticotropin-releasing factor receptor expression in normal cycling human ovaries. , 2001, The Journal of clinical endocrinology and metabolism.

[33]  A. Abou-Samra,et al.  Characterization of Three Corticotropin-Releasing Factor Receptors in Catfish: A Novel Third Receptor Is Predominantly Expressed in Pituitary and Urophysis*† * The nucleotide sequences reported in this paper have been submitted to the GenBank database with accession numbers cfCRF-R1, AF229359; cfCRF , 2001, Endocrinology.

[34]  J. Vaughan,et al.  Urocortin Expression in Rat Brain: Evidence Against a Pervasive Relationship of Urocortin‐Containing Projections With Targets Bearing Type 2 CRF Receptors , 1999, The Journal of comparative neurology.

[35]  Xin-wei Lin,et al.  Differential expression of corticotropin-releasing factor (CRF) and urotensin I precursor genes, and evidence of CRF gene expression regulated by cortisol in goldfish brain. , 1999, General and comparative endocrinology.

[36]  H. Sarau,et al.  Human urotensin-II is a potent vasoconstrictor and agonist for the orphan receptor GPR14 , 1999, Nature.

[37]  D. Lovejoy,et al.  Evolution and physiology of the corticotropin-releasing factor (CRF) family of neuropeptides in vertebrates. , 1999, General and comparative endocrinology.

[38]  W. Goebel,et al.  Differential expression of , 1999 .

[39]  Kazuhiro Takahashi,et al.  Regional Distribution of Urocortin-like Immunoreactivity and Expression of Urocortin mRNA in the Human Brain , 1998, Peptides.

[40]  W. Dickhoff,et al.  In vitro thyrotropin-releasing activity of corticotropin-releasing hormone-family peptides in coho salmon, Oncorhynchus kisutch. , 1998, General and comparative endocrinology.

[41]  David Lovejoy,et al.  Urocortin, a mammalian neuropeptide related to fish urotensin I and to corticotropin-releasing factor , 1995, Nature.

[42]  M. Rose.  Evolution and physiology. , 1991, Science.

[43]  J. Rivier,et al.  Urotensin I, a CRF-like neuropeptide, stimulates acth release from the teleost pituitary. , 1983, Endocrinology.

[44]  G. Moore,et al.  Complete amino acid sequence of urotensin I, a hypotensive and corticotropin-releasing neuropeptide from Catostomus. , 1982, Science.