Multimodal Imaging Based on Digital Holography

Digital holography provides a method of the 3-D recording and numerical reconstruction by a simple optical system and a computer. Quantitative measurement and numerical refocusing are major characteristics. So far, many physical parameters such as amplitude, phase, polarization, fluorescence, and spectra can be obtained independently. Recently, multimodal imaging that can obtain simultaneously two or more physical parameters by combining digital holographic microscope and other optical microscopes such as a fluorescence optical microscope and a Raman scattering microscope has emerged. In this review, physical parametric imaging techniques based on digital holography are presented and then these techniques are enhanced to develop multimodal imaging based on digital holography.