Diffuse reflectance spectroscopy for Onchocerca Volvulus nodules characterization

We show the potential of Diffuse Reflectance Spectroscopy to identify Onchocerca Volvulus nodules, cysts, ganglions and lipomas on humans. We develop a new probe to take into account the geometry of the O. Volvulus model and implement a new measurement protocol and analysis method to deal with the melanin skin contribution. The nodule has a specific intensity and spectral signature compared to its background. It is therefore a relative measure that estimates a signal variation due to the worm and its environment. This may be used to monitor the worm state along time.