A new amperometric enzyme electrode for alcohol determination.

A new enzyme electrode for the determination of alcohols was developed by immobilizing alcohol oxidase in polvinylferrocenium matrix coated on a Pt electrode surface. The amperometric response due to the electrooxidation of enzymatically generated H(2)O(2) was measured at a constant potential of +0.70 V versus SCE. The effects of substrate, buffer and enzyme concentrations, pH and temperature on the response of the electrode were investigated. The optimum pH was found to be pH 8.0 at 30 degrees C. The steady-state current of this enzyme electrode was reproducible within +/-5.0% of the relative error. The sensitivity of the enzyme electrode decreased in the following order: methanol>ethanol>n-butanol>benzyl alcohol. The linear response was observed up to 3.7 mM for methanol, 3.0 mM for ethanol, 6.2 mM for n-butanol, and 5.2 mM for benzyl alcohol. The apparent Michaelis-Menten constant (K(Mapp)) value and the activation energy, E(a), of this immobilized enzyme system were found to be 5.78 mM and 38.07 kJ/mol for methanol, respectively.

[1]  W. A. Scheffers,et al.  A simple, sensitive, and accurate alcohol electrode , 1983, Biotechnology and bioengineering.

[2]  D. D. Perrin,et al.  Purification of laboratory chemicals , 1966 .

[3]  M. Mason,et al.  Ethanol Determination in Wine with an Immobilized Enzyme Electrode , 1983, American Journal of Enology and Viticulture.

[4]  Dario Compagnone,et al.  Alcohol Electrodes in Beverage Measurements , 1994 .

[5]  Soichi Yabuki,et al.  Amperometric Alcohol-Sensing Electrode Based on a Polyion Complex Membrane Containing Alcohol Oxidase , 1997 .

[6]  J. Wangsa,et al.  Enzymatic determination of ethanol by flow injection analysis using a Kel-F wax carbon paste electrode , 1991 .

[7]  G. Inzelt,et al.  Electrochemical quartz crystal microbalance study of the swelling of poly(vinylferrocene) films , 1992 .

[8]  J. Kulys,et al.  BIENZYME SENSORS BASED ON CHEMICALLY MODIFIED ELECTRODES , 1991 .

[9]  G. Palleschi,et al.  Amperometric alcohol electrode with extended linearity and reduced interferences. , 1991, Analytical biochemistry.

[10]  R. Buck,et al.  An all-solid-state amperometric ethanol sensor , 1991 .

[11]  H. Gülce,et al.  A new amperometric enzyme electrode for galactose determination , 2002 .

[12]  Thomas W. Smith,et al.  Voltammetric behavior of poly(vinylferrocene) , 1976 .

[13]  Arturo J. Miranda,et al.  A comparative study of some phenoxazine and phenothiazine modified carbon paste electrodes for ethanol determination , 1996 .

[14]  P. Blanco,et al.  Amperometric detection of ethanol with poly-(o-phenylenediamine)-modified enzyme electrodes , 1997 .

[15]  M. Mascini,et al.  Microbial sensor for alcohol , 1989 .

[16]  S. Çelebi,et al.  Amperometric enzyme electrode for sucrose determination prepared from glucose oxidase and invertase co-immobilized in poly(vinylferrocenium) , 1995 .

[17]  A. Bard,et al.  Polymer films on electrodes: Part II. Film structure and mechanism of electron transfer with electrodeposited poly(vinylferrocene) , 1980 .

[18]  S. Çelebi,et al.  Amperometric enzyme electrode for aerobic glucose monitoring prepared by glucose oxidase immobilized in poly(vinylferrocenium) , 1995 .