Theoretical and practical aspects of glutaraldehyde fixation

SynopsisThis review first considers the many structures put forward for glutaraldehyde, and the purification of the commercial material for chemical, histological and histochemical studies. Some practical and theoretical problems of tissue fixation with glutaraldehyde, including artefacts, are then discussed. The chemical reactions with amino acids and proteins are considered next together with the physical changes in the proteins during the reactions. The known reactions of glutaraldehyde with nucleic acids, lipids and mucosubstances are explored briefly.

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