Phenotypic identification of plum varieties (Prunus domestica L.) by endocarps morpho-colorimetric and textural descriptors

P. domestica endocarps biometric features were measured by image analysis techniques.A total of 134 size, shape, color and texture descriptors were measured on each endocarp.The data were statistically processed applying the stepwise Linear Discriminant Analysis.Hypothetical synonymy groups were identified.This is the first computer vision application on plum biodiversity study. The identification of plum varieties is generally done on the base of distinctive plant traits such as shape, size, and fruit drupe color identified during the variety registration, following official descriptors. In this paper, image analysis techniques were applied to study endocarps variability of 23 Prunus domestica cultivars from Sardinia. Digital images were acquired and analysed using a macro specifically developed to measure morpho-colorimetric endocarps features. The data were later statistically processed applying the stepwise Linear Discriminant Analysis (LDA) to implement a statistical classifier able to classify each variety and identify plausible synonymy groups.The present study represent the first attempt to investigate the morphology and morphometry of plum endocarps in order to characterize the whole Sardinian plum agrobiodiversity. It is also the evidence of the usefulness of image analysis techniques in taxonomic investigations too, as well as for the conservation and enhancement of traditional plums for consumer satisfaction.

[1]  M. Faust,et al.  Origin and Dissemination of Plums , 2010 .

[2]  O. Grillo,et al.  Morpho-colorimetric analysis and seed germination of Brassica insularis Moris (Brassicaceae) populations. , 2015, Plant biology.

[3]  Gianluigi Bacchetta,et al.  Effectiveness of a computer vision technique in the characterization of wild and farmed olives , 2016, Comput. Electron. Agric..

[4]  R. Fisher THE PRECISION OF DISCRIMINANT FUNCTIONS , 1940 .

[5]  O. Grillo,et al.  Predictive Method for Correct Identification of Archaeological Charred Grape Seeds: Support for Advances in Knowledge of Grape Domestication Process , 2016, PloS one.

[6]  N. Fawzi Macro-and Micromorphological Seed Characteristics of some Selected Species of Caesalpinioideae-Leguminosae , 2011 .

[7]  Robert M. Haralick,et al.  Textural Features for Image Classification , 1973, IEEE Trans. Syst. Man Cybern..

[8]  O. Grillo,et al.  Inter- and intraspecific morphometric variability in Juniperus L. seeds (Cupressaceae) , 2014 .

[9]  O. Grillo,et al.  Productive, qualitative and seed image analysis traits of guar (Cyamopsis tetragonoloba L. Taub.) , 2016 .

[10]  Computer vision as a method complementary to molecular analysis: grapevine cultivar seeds case study. , 2012, Comptes rendus biologies.

[11]  Stephen J. Symons,et al.  Color Calibration of Scanners for Scanner‐Independent Grain Grading , 2003 .

[12]  Geographic isolation affects inter- and intra-specific seed variability in the Astragalus tragacantha complex, as assessed by morpho-colorimetric analysis. , 2013, Comptes rendus biologies.

[13]  M. Kuhn,et al.  Discriminant Analysis and Other Linear Classification Models , 2013 .

[14]  O. Grillo,et al.  Germplasm image analysis of Astragalus maritimus and A. verrucosus of Sardinia (subgen. Trimeniaeus , Fabaceae) , 2008 .

[15]  O. Grillo,et al.  Morpho-colorimetric traits of Pisum seeds measured by an image analysis system , 2011 .

[16]  T. Lacombe,et al.  Bioarchaeological Insights into the Process of Domestication of Grapevine (Vitis vinifera L.) during Roman Times in Southern France , 2013, PloS one.

[17]  O. Grillo,et al.  Earliest evidence of a primitive cultivar of Vitis vinifera L. during the Bronze Age in Sardinia (Italy) , 2015, Vegetation History and Archaeobotany.

[18]  V. Usenik,et al.  Anthocyanins and fruit colour in plums (Prunus domestica L.) during ripening. , 2009 .

[19]  F. G. Meyer,et al.  The natural history of Pompeii , 2002 .

[20]  O. Grillo,et al.  Morpho-colorimetric characterization by image analysis to identify diaspores of wild plant species , 2008 .

[21]  P. Goetghebeur,et al.  Stony endocarp dimension and shape variation in prunus section prunus. , 2007, Annals of botany.

[22]  O. Grillo,et al.  Seeds morpho-colourimetric analysis as complementary method to molecular characterization of melon diversity , 2015 .

[23]  F. Gyulai,et al.  Computer-assisted morphometry: A new method for assessing and distinguishing morphological variation in wild and domestic seed populations , 2007, Economic Botany.

[24]  Robert M. Haralick,et al.  Glossary of computer vision terms , 1990, Pattern Recognit..

[25]  O. Hâruța Elliptic Fourier analysis of crown shapes in Quercus petraea trees. , 2011 .

[26]  Robert Tibshirani,et al.  The Elements of Statistical Learning: Data Mining, Inference, and Prediction, 2nd Edition , 2001, Springer Series in Statistics.

[27]  Gianluigi Bacchetta,et al.  Statistical seed classifiers of 10 plant families representative of the Mediterranean vascular flora. , 2010 .

[28]  O. Grillo,et al.  Seed image analysis and taxonomy of Diplotaxis DC. (Brassicaceae, Brassiceae) , 2012 .

[29]  Maria Hopf,et al.  Domestication of plants in the Old World. The origin and spread of cultivated plants in West Asia, Europe and the Nile Valley. , 2000 .

[30]  C. Pontecorvo,et al.  La Banca del Germoplasma della Sardegna (BG-SAR) , 2005 .

[31]  Masashi Sugiyama,et al.  Dimensionality Reduction of Multimodal Labeled Data by Local Fisher Discriminant Analysis , 2007, J. Mach. Learn. Res..

[32]  I. Athanasiadis,et al.  Genetic relatedness among cultivars of the Greek plum germplasm. , 2013 .

[33]  Britta Pollmann,et al.  Morphological and genetic studies of waterlogged Prunus species from the Roman vicus Tasgetium (Eschenz, Switzerland) , 2005 .

[34]  Smýkalová Iva,et al.  Phenotypic evaluation of flax seeds by image analysis , 2013 .

[35]  R. Fisher THE USE OF MULTIPLE MEASUREMENTS IN TAXONOMIC PROBLEMS , 1936 .

[36]  F. Laigret,et al.  Phenotypic variability and genetic structure in plum (Prunus domestica L.), cherry plum (P. cerasifera Ehrh.) and sloe (P. spinosa L.) , 2011 .

[37]  G. Venora,et al.  Computerised image analysis applied to inspection of vetch seeds for varietal identification , 2011 .

[38]  O. Grillo,et al.  Characterisation of Italian bean landraces ('Phaseolus vulgaris' L.) using seed image analysis and texture descriptors , 2015 .

[39]  O. Grillo,et al.  Seed image analysis provides evidence of taxonomic differentiation within the Medicago L. sect. Dendrotelis (Fabaceae) , 2015 .

[40]  D. C. Olrik,et al.  A morphometric analysis of Prunus spinosa, P. domestica ssp. insititia, and their putative hybrids in Denmark , 2001 .

[41]  O. Grillo,et al.  Seed morpho‐colorimetric analysis by computer vision: a helpful tool to identify grapevine (Vitis vinifera L.) cultivars , 2015 .

[42]  Ken Kelley,et al.  A Comparison of Two-Group Classification Methods , 2011 .