Expression of interleukin‐2 receptor γ on human monocytes: characterization of lineage specific post‐translational modifications

Functional interleukin‐2 receptors (IL‐2R) on lymphocytes contain both IL‐2R β and γ chains. Whereas constitutive expression of IL‐2Rβ has been found on monocytes, the expression of IL‐2Rγ on these phagocytes has not been examined. We performed reverse‐transcription‐polymerase chain reaction with Southern blot analysis on RNA derived from purified human monocytes and discovered that they constitutively produce IL‐2Rγ mRNA. Western immunoblotting revealed 58‐and 64‐kDa forms of IL‐2Rγ on YT‐1 and human monocytes, whereas 58‐, 64‐, and 69‐kDa bands were detected using peripheral blood mononuclear cells and non‐adherent lymphocytes. These different forms resulted from variable N‐linked glycosylation since culture of the cells in tunicamycin resulted in detection of a single 39‐kDa band which corresponds to the molecular weight predicted from the deduced amino acid sequence. By co‐immunoprecipitation, the IL‐2Rβ subunit associates with only the 64‐kDa IL‐2Rγ protein band in monocytes.

[1]  B. Nelson,et al.  Cytoplasmic domains of the interleukin-2 receptor β and γ chains mediate the signal for T-cell proliferation , 1994, Nature.

[2]  S. Ziegler,et al.  Interleukin-2 receptor gamma chain: a functional component of the interleukin-7 receptor. , 1993, Science.

[3]  K. Arai,et al.  Sharing of the interleukin-2 (IL-2) receptor gamma chain between receptors for IL-2 and IL-4. , 1993, Science.

[4]  W. Leonard,et al.  Characterization of the human interleukin-2 receptor gamma chain gene. , 1993, The Journal of biological chemistry.

[5]  D. Blanchard,et al.  Coinduction of granulocyte-macrophage colony-stimulating factor release and lymphokine-activated killer cell susceptibility in monocytes by interleukin-2 via interleukin-2 receptor beta. , 1993, Blood.

[6]  H. Asao,et al.  Cloning of the mouse interleukin 2 receptor gamma chain: demonstration of functional differences between the mouse and human receptors. , 1993, Biochemical and biophysical research communications.

[7]  W. Leonard,et al.  Interleukin-2 receptor γ chain mutation results in X-linked severe combined immunodeficiency in humans , 1993, Cell.

[8]  T. Leary,et al.  Identification of a direct interaction between interleukin 2 and the p64 interleukin 2 receptor gamma chain. , 1993, Proceedings of the National Academy of Sciences of the United States of America.

[9]  W. Leonard,et al.  Activation of tumor necrosis factor-alpha production from human neutrophils by IL-2 via IL-2-R beta. , 1993, Journal of immunology.

[10]  F. Balkwill,et al.  Temporal sequence and cellular origin of interleukin-2 stimulated cytokine gene expression. , 1993, British Journal of Cancer.

[11]  S. Voss,et al.  Characterization of the interleukin 2 receptors (IL-2R) expressed on human natural killer cells activated in vivo by IL-2: association of the p64 IL-2R gamma chain with the IL-2R beta chain in functional intermediate-affinity IL-2R , 1992, The Journal of experimental medicine.

[12]  N. Tanaka,et al.  Cloning of the gamma chain of the human IL-2 receptor. , 1992, Science.

[13]  H. Asao,et al.  IL‐2‐dependent in vivo and in vitro tyrosine phosphorylation of IL‐2 receptor γ chain , 1992 .

[14]  H. Asao,et al.  An associated molecule, p64, with IL-2 receptor beta chain. Its possible involvement in the formation of the functional intermediate-affinity IL-2 receptor complex. , 1992, Journal of immunology.

[15]  D. Longo,et al.  IL-2 induces IL-6 production in human monocytes. , 1992, Journal of immunology.

[16]  A. Kobata Structures and functions of the sugar chains of glycoproteins. , 1988, European journal of biochemistry.

[17]  P. Weller,et al.  Human eosinophils express functional interleukin 2 receptors. , 1991, The Journal of clinical investigation.

[18]  D. Longo,et al.  IL-2 enhances c-fms expression in human monocytes. , 1990, Journal of immunology.

[19]  D. Longo,et al.  Expression and role of p75 interleukin 2 receptor on human monocytes , 1990, The Journal of experimental medicine.

[20]  T. Taniguchi,et al.  Interleukin-2 receptor beta chain gene: generation of three receptor forms by cloned human alpha and beta chain cDNA's. , 1989, Science.

[21]  P. Chomczyński,et al.  Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. , 1987, Analytical biochemistry.

[22]  W. Fiers,et al.  Recombinant interleukin-2 directly augments the cytotoxicity of human monocytes , 1987, Nature.

[23]  G. Crabtree,et al.  Molecular cloning and expression of cDNAs for the human interleukin-2 receptor , 1984, Nature.

[24]  U. K. Laemmli,et al.  Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4 , 1970, Nature.