Chemical composition and antioxidant activity of Coccoloba cowellii Britton

The qualitative and quantitative chemical composition (total phenolics, tannins and flavonoids contents), as well as the antioxidant activity of an ethanolic extract of the leaves of Coccoloba cowellii (Polygonaceae) was determined in this study. The extract was prepared by dynamic ultrasound-assisted extraction using firstly petroleum ether:ethyl ether (1:1, v/v) and therefore ethanol:water (8:2, v/v). Quantitative chemical composition (total phenolics, tannins and flavonoids content) was determined using spectroscopic techniques, while the antioxidant activity was determined by means of the 1,1-diphenyl-2-picryl-hydrazyl (DPPH) and ferric reducing power tests. The ethanolic extract showed significant antioxidant activity in both assays, with values of 34,01 % ± 4,03 % in the DPPH and reduced Fe ferricyanide complex to the ferrous form (Fe), similar to the ascorbic acid used as standard in both assays. The antioxidant activity of the extract is consistent with the total phenolic, tannins and flavonoid contents of the extract.

[1]  M. R. García-Mateos,et al.  Phenolic Compounds - Biological Activity , 2017 .

[2]  Lawson-evi Povi,et al.  PHYTOCHEMICAL SCREENING, ANTIOXIDANT AND HYPOGLYCEMIC ACTIVITY OF COCCOLOBA UVIFERA LEAVES AND WALTHERIA INDICA ROOTS EXTRACTS , 2015 .

[3]  D. Ancona,et al.  Coccoloba uvifera as a source of components with antioxidant activity , 2015 .

[4]  Giovanni Agati,et al.  Flavonoids as antioxidants in plants: location and functional significance. , 2012, Plant science : an international journal of experimental plant biology.

[5]  A Marston,et al.  Thin-layer chromatography with biological detection in phytochemistry. , 2011, Journal of chromatography. A.

[6]  G. Sudha,et al.  IN VITRO FREE RADICAL SCAVENGING ACTIVITY OF RAW PEPINO FRUIT (SOLANUM MURICATUM AITON) , 2011 .

[7]  I. Kovalchuk.  Multiple Roles of Radicals in Plants , 2010 .

[8]  Q. Su,et al.  Dynamic ultrasound-assisted extraction of catechins and caffeine in some tea samples. , 2007, Annali di chimica.

[9]  Jin-Yuarn Lin,et al.  DETERMINATION OF TOTAL PHENOLIC AND FLAVONOID CONTENTS IN SELECTED FRUITS AND VEGETABLES, AS WELL AS THEIR STIMULATORY EFFECTS ON MOUSE SPLENOCYTE PROLIFERATION , 2007 .

[10]  R. Arora,et al.  Herbal Drugs. A Twenty First Century Perspective , 2006 .

[11]  O. Aruoma,et al.  Phenolics as potential antioxidant therapeutic agents: mechanism and actions. , 2005, Mutation research.

[12]  Á. María.,et al.  Extracción de taninos presentes en el banano verde , 2004 .

[13]  F. Braga,et al.  Antimicrobial activity and constituents of Coccoloba acrostichoides. , 2003, Fitoterapia.

[14]  H. Makkar.  Quantification of Tannins in Tree and Shrub Foliage , 2003, Springer Netherlands.

[15]  G. Ilavazhagan,et al.  Evaluation of antioxidant activity of leaf extract of Seabuckthorn (Hippophae rhamnoides L.) on chromium(VI) induced oxidative stress in albino rats. , 2003, Journal of ethnopharmacology.

[16]  G. Pérez,et al.  Antimicrobial study of bark from five tree species , 2001 .

[17]  H. Elsohly,et al.  Antifungal activity of (-)-epigallocatechin gallate from Coccoloba dugandiana. , 1999, Planta medica.

[18]  E. Baldwin,et al.  Volatile constituents of Coccolobo uvifera , 1992 .

[19]  M. Oyaizu.  Studies on products of browning reaction--antioxidative activities of products of browning reaction prepared from glucosamine , 1986 .

[20]  S. Dan.  Phytochemical study of Adansonia digitata, Coccoloba excoriata, Psychotria adenophylla and Schleichera oleosa. , 1986 .

[21]  E. Harris,et al.  Attraction of ethyl ether extracts of 232 botanicals to oriental fruit flies, melon flies, and Mediterranean fruit flies. , 1975, Lloydia.

[22]  M. S. Blois,et al.  Antioxidant Determinations by the Use of a Stable Free Radical , 1958, Nature.