Sequence similarity of human caliciviruses and small round structured viruses

The application of reverse transcription‐polymerase chain reaction (RT‐PCR) using primers directed to the RNA dependent RNA polymerase region within ORF1 of Norwalk virus (NV) showed that 31 percent of morphologically typical human caliciviruses (HuCV) and 57% of small round structured viruses (SRSVs) produced a product of 470 bp similar to the NV control, NV 8Flla/68/US. Alignment of the amino acid sequences of morphologically typical HuCVs with previously published sequences for SRSVs, NV, and Snow Mountain agent (SMA) showed a high degree of homology (90–92%) with SMA and a lesser extent of homology with NV (60–61%). The amino acid sequence of two strains of HuCV, HuCV/3C/92/UK, and HuCV/5C/92/UK differed by only one or two amino acids respectively in the RNA dependent RNA polymerase region from that of two strains of SRSV obtained from children in the United Kingdom, SRSV/4S/90/UK and Japan, SRSV/OTH‐25/89/J which were found to have identical amino acid sequences. The use of an EIA for detection of NV antigen employing antisera raised to recombinant NV protein indicated that HuCVs and SRSVs obtained from children and adults in the United Kingdom were antigenically distinct from the prototype Norwalk Virus, NV/8flla/68/US. © 1994 Wiley‐Liss, Inc.

[1]  W. Cubitt.  The candidate caliciviruses. , 2007, Ciba Foundation symposium.

[2]  J. Gray,et al.  Sequence diversity of small, round-structured viruses in the Norwalk virus group , 1994, Journal of virology.

[3]  M. Estes,et al.  Seroprevalence studies using a recombinant norwalk virus protein enzyme immunoassay , 1994, Journal of medical virology.

[4]  M. Estes,et al.  Two successive outbreaks of SRSV‐associated gastroenteritis in South Africa , 1993, Journal of medical virology.

[5]  M. Carter,et al.  Detection of Norwalk virus in the UK by the polymerase chain reaction. , 1993, FEMS microbiology letters.

[6]  M. Estes,et al.  Sequence and genomic organization of Norwalk virus. , 1993, Virology.

[7]  M. Estes,et al.  Prevalence of antibodies to Norwalk virus in England: detection by enzyme-linked immunosorbent assay using baculovirus-expressed Norwalk virus capsid antigen , 1993, Journal of clinical microbiology.

[8]  I. Clarke,et al.  Sequence and genome organization of a human small round-structured (Norwalk-like) virus. , 1993, Science.

[9]  A. Lavazza,et al.  Haemorrhagic disease of lagomorphs: evidence for a calicivirus. , 1992, Veterinary microbiology.

[10]  J. Treanor,et al.  Antigenic relatedness among the Norwalk‐like agents by serum antibody rises , 1990, Journal of medical virology.

[11]  R. Glass,et al.  Human Calicivirus-Associated Diarrhea In Children Attending Day Care Centers , 1989, The Journal of infectious diseases.

[12]  D. Bradley,et al.  Aetiological agent of enterically transmitted non-A, non-B hepatitis. , 1988, The Journal of general virology.

[13]  J. Herrmann,et al.  Antigenic relationships between human caliciviruses and Norwalk virus. , 1987, The Journal of infectious diseases.

[14]  J. Treanor,et al.  Characterization of the Snow Mountain agent of viral gastroenteritis , 1986, Journal of virology.

[15]  R. Chanock,et al.  Proteins of Norwalk virus , 1981, Journal of virology.

[16]  W. Cubitt,et al.  Winter vomiting disease caused by calicivirus. , 1979, Journal of clinical pathology.

[17]  B. Cosgrove,et al.  CALICIVIRUSES IN MAN , 1976, The Lancet.

[18]  R G Wyatt,et al.  Visualization by Immune Electron Microscopy of a 27-nm Particle Associated with Acute Infectious Nonbacterial Gastroenteritis , 1972, Journal of virology.

[19]  F. L. Schaffer,et al.  Biochemical and biophysical properties of vesicular exanthema of swine virus. , 1971, Virology.

[20]  D. Bradley.  Enterically-transmitted non-A, non-B hepatitis. , 1990, British medical bulletin.

[21]  H. Appleton,et al.  The electron microscopical and physical characteristics of small round human fecal viruses: An interim scheme for classification , 1982, Journal of medical virology.