Manipulation and injection of a specific magnetic nanosensor using optical zeta potential control and local laser heating

Effective manipulation and rapid injection of nanosensor with high cell viability have great significance to biological and biomedical applications such as drug delivery and cells biology. This study presents manipulation of a magnetic nanosensor, immobilization onto cell surface using zeta potential control and injection into cell cytoplasm by local laser heating. The nanosensor is stained by a temperature indicator and coated with lipid layer containing photochromic material. The zeta potential of the liposome is controlled interchangeability between positive by UV illumination and negative by VIS illumination. The positively charged nanosensor is manipulated by glass nanoprobe and immobilized on the cell membrane since the charge of the cells are negative. We demonstrated the manipulation and immobilization of the nanosensor using optical charge control and injection of the sensor into the cell cytoplasm by local laser heating.