Composition and Structural Characteristics of Rhizosphere Microorganisms of Polygonum sibiricum (Laxm.) Tzvelev in the Yellow River Delta

The Polygonum sibiricum (Laxm.) Tzvelev, an important herbal species used to protect seawalls, has a solid resistance to salinity and alkali and can grow on alkali spots in saline–alkali soils. So far, the composition, population, and characteristics of its rhizosphere biological community related to the adaptation salt–alkali environment were still unknown. In the present study, rhizosphere and non-rhizosphere soil samples from the P. sibiricum on Chenier Island were collected. High-throughput sequencing was conducted to obtain the structural diversity of rhizosphere microbial communities. Our results showed that the dominant bacteria groups in the rhizosphere and non-rhizosphere were Proteobacteria, Actinobacteriota, Gemmatimonadota, and Actinobacteriota. The dominant fungi groups in the rhizosphere and non-rhizosphere soil samples were Ascomycota, Basidiomycota, Chytridiomycota, and Mortierellomycota. The results of the ASVs (amplicon sequence variants) showed that fungi have more ASVs in common. The PERMANOVA analysis showed that the bacteria among different groups were significantly different. The PCoA (principal coordinates analysis) study also showed that the structures of the bacterial and fungal communities between the rhizosphere and non-rhizosphere were distinct. Function results showed that the relative abundance in COG (Clusters of Orthologous Groups of proteins) functional annotation was significantly different between the two groups. In addition to the general function prediction and carbohydrate transport and metabolism, the COG of the non-rhizosphere was higher than that of the rhizosphere. Our findings benefited the knowledge for studying and conserving the molecule-level adaptive mechanisms of P. sibiricum.

[1]  J. Vivanco,et al.  The rhizosphere microbiome: Plant–microbial interactions for resource acquisition , 2022, Journal of applied microbiology.

[2]  Xue-Xi Tang,et al.  Effects of Spartina alterniflora invasion on the community structure and diversity of wetland soil bacteria in the Yellow River Delta , 2022, Ecology and evolution.

[3]  M. Koblížek,et al.  Phylum Gemmatimonadota and Its Role in the Environment , 2022, Microorganisms.

[4]  C. Granada,et al.  Soil-plant-microbiota interactions to enhance plant growth , 2022, Revista Brasileira de Ciência do Solo.

[5]  Jun Chen,et al.  D-MANOVA: fast distance-based multivariate analysis of variance for large-scale microbiome association studies , 2021, Bioinform..

[6]  Andrea Telatin,et al.  Qiime Artifact eXtractor (qax): A Fast and Versatile Tool to Interact with Qiime2 Archives , 2021, Biotech (Basel (Switzerland)).

[7]  Tsuyoshi Kobayashi,et al.  Influence of historical inundation frequency on soil microbes (Cyanobacteria, Proteobacteria, Actinobacteria) in semi-arid floodplain wetlands , 2020 .

[8]  Jian-Ping Hong,et al.  Contrast in soil microbial metabolic functional diversity to fertilization and crop rotation under rhizosphere and non-rhizosphere in the coal gangue landfill reclamation area of Loess Hills , 2020, PloS one.

[9]  Yuzhe Wang,et al.  Response of Bacterial and Fungal Soil Communities to Chinese Fir (Cunninghamia lanceolate) Long-Term Monoculture Plantations , 2020, Frontiers in Microbiology.

[10]  C. Ryu,et al.  Inheritance of seed and rhizosphere microbial communities through plant-soil feedback and soil memory. , 2019, Environmental microbiology reports.

[11]  Y. H. Zhao,et al.  Soil microbial community structure in the rhizosphere of Robinia pseudoacacia L. seedlings exposed to elevated air temperature and cadmium-contaminated soils for 4 years. , 2019, The Science of the total environment.

[12]  U. Stingl,et al.  The Microbial Communities of Leaves and Roots Associated with Turtle Grass (Thalassia testudinum) and Manatee Grass (Syringodium filliforme) are Distinct from Seawater and Sediment Communities, but Are Similar between Species and Sampling Sites , 2018, Microorganisms.

[13]  S. M. Mousavi,et al.  Root-induced changes of Zn and Pb dynamics in the rhizosphere of sunflower with different plant growth promoting treatments in a heavily contaminated soil. , 2018, Ecotoxicology and environmental safety.

[14]  Hua-song Wu,et al.  Different responses of rhizosphere and non-rhizosphere soil microbial communities to consecutive Piper nigrum L. monoculture , 2016, Scientific Reports.

[15]  Paul J. McMurdie,et al.  DADA2: High resolution sample inference from Illumina amplicon data , 2016, Nature Methods.

[16]  G. Muyzer,et al.  Rhizosphere Microbiomes of European Seagrasses Are Selected by the Plant, But Are Not Species Specific , 2016, Front. Microbiol..

[17]  Anders F. Andersson,et al.  Experimental insights into the importance of aquatic bacterial community composition to the degradation of dissolved organic matter , 2015, The ISME Journal.

[18]  Susan Holmes,et al.  phyloseq: An R Package for Reproducible Interactive Analysis and Graphics of Microbiome Census Data , 2013, PloS one.

[19]  Yuan Zhang,et al.  Involvement of auxin and nitric oxide in plant Cd-stress responses , 2011, Plant and Soil.

[20]  Eoin L. Brodie,et al.  Differential Growth Responses of Soil Bacterial Taxa to Carbon Substrates of Varying Chemical Recalcitrance , 2011, Front. Microbio..

[21]  Chun Liu,et al.  Differential Expression of Copper-Zinc Superoxide Dismutase Gene of Polygonum sibiricum Leaves, Stems and Underground Stems, Subjected to High-Salt Stress , 2010, International journal of molecular sciences.

[22]  E. Patkowska.  Effect of Bio-Products on Bean Yield and Bacterial and Fungal Communities in the Rhizosphere and Non-Rhizosphere , 2009 .

[23]  S. Gnanamanickam.  Plant: Associated Bacteria , 2008 .

[24]  P. Garbeva,et al.  Rhizosphere microbial community and its response to plant species and soil history , 2007, Plant and Soil.

[25]  L. Zhi-jie,et al.  The sedimentary characteristics and formation mechanism of shell ridges along the southwest coast of Bohai Bay , 2005 .