论文信息 - ON THE WAY TO A FULLY INTEGRATED DNA-PURIFICATION SYSTEM ON A STANDARD LABORATORY CENTRIFUGE

ON THE WAY TO A FULLY INTEGRATED DNA-PURIFICATION SYSTEM ON A STANDARD LABORATORY CENTRIFUGE

For the first time, we combine liquid reagent storage in glass capillaries [1], time-controlled reagent release [2], and a novel solution for unidirectional centrifugal routing, paving the way to a fully integrated DNA extraction on a standard laboratory centrifuge. The average yield of the extraction is 192 ± 30 ng DNA out of 32 μl blood, corresponding to 53 ± 8 % of a reference extraction. The overall processing time of ~66 minutes can be reduced to ~8 minutes after optimization of the burst-valves. This novel approach demonstrates a convenient way for fully automated DNAextraction on a standard laboratory centrifuge.

[1] Roland Zengerle,et al. MICROFLUIDIC UNIDIRECTIONAL PNEUMATIC SWITCH FOR AUTOMATED DNA-EXTRACTION ON STANDARD LABORATORY CENTRIFUGES , 2009 .

[2] R. Zengerle,et al. Liquid reagent storage and release for centrifugally operated Lab-on-a-Chip systems based on a burstable seal , 2009, TRANSDUCERS 2009 - 2009 International Solid-State Sensors, Actuators and Microsystems Conference.

[3] Roland Zengerle,et al. Pre-storage of liquid reagents in glass ampoules for DNA extraction on a fully integrated lab-on-a-chip cartridge. , 2010, Lab on a chip.