Selective removal of 17β-estradiol at trace concentration using a molecularly imprinted polymer

A molecularly imprinted polymer (MIP) was synthesized with 17 beta-estradiol (E2) as template. It was then capable to recover this compound by 100 +/- 0.6% from a 2 mu g/L aqueous solution. By comparison, E2 recoveries of 77 +/- 5.2%, 87.1 +/- 2.3% and 19.1 +/- 7.8%, were achieved using a non-imprinted polymer (NIP) synthesized under the same conditions (but without template), a commercial C18 extraction phase and granular-activated carbon (GAC), respectively When fluoxetine hydrochloride and acenaphthene were added as interferences to the aqueous solution at 2 mu g/L each, E2 was recovered by 95.5 +/- 4.0% from the MIP, compared to 54.5 +/- 9.4%, 76.0 +/- 2% and 14.3 +/- 0.1% from the NIP, C18 and GAC phases, respectively Estrogenic activity equivalent to the effect caused by 22.4ng E2/L was recorded in the MIP extract from a wastewater sample whereas no activity was detected in the NIP extract. This suggested the imprinted polymers removed estrogenic compounds. This study therefore demonstrates the potential of MIPs for the selective removal of endocrine-disrupting compounds. By using a synthetic analogue to natural hormone receptors, adsorption is based on the same property that makes the contaminants harmful. Biological treatment of enriched E2 was also demonstrated. (c) 2007 Elsevier Ltd. All rights reserved. (Less)

[1]  T. Ternes,et al.  Pharmaceuticals and personal care products in the environment: agents of subtle change? , 1999, Environmental health perspectives.

[2]  John P. Sumpter,et al.  Widespread Sexual Disruption in Wild Fish , 1998 .

[3]  J. Santodonato.  Review of the estrogenic and antiestrogenic activity of polycyclic aromatic hydrocarbons: relationship to carcinogenicity. , 1997, Chemosphere.

[4]  Craig D. Adams,et al.  Endocrine disrupting compounds removal from wastewater, a new challenge , 2006 .

[5]  F. Borrull,et al.  Molecularly imprinted polymers: new tailor-made materials for selective solid-phase extraction , 2001 .

[6]  Frank Sacher,et al.  Removal of pharmaceuticals during drinking water treatment. , 2002, Environmental science & technology.

[7]  J. Fawell,et al.  Oestrogens and oestrogenic activity in raw and treated water in Severn Trent Water. , 2001, Water research.

[8]  Javier Pérez-Ramírez,et al.  Pore size determination in modified micro- and mesoporous materials. Pitfalls and limitations in gas adsorption data analysis , 2003 .

[9]  Wilfred Chen,et al.  Removal of estrogenic pollutants from contaminated water using molecularly imprinted polymers. , 2005, Environmental science & technology.

[10]  C. Tyler,et al.  Comparative responses of molluscs and fish to environmental estrogens and an estrogenic effluent. , 2003, Aquatic toxicology.

[11]  R J Golden,et al.  Environmental endocrine modulators and human health: an assessment of the biological evidence. , 1998, Critical reviews in toxicology.

[12]  B. Mattiasson,et al.  Removal of trace contaminants using molecularly imprinted polymers. , 2006, Water science and technology : a journal of the International Association on Water Pollution Research.

[13]  Peter Seto,et al.  Distribution of estrogens, 17β-estradiol and estrone, in Canadian municipal wastewater treatment plants , 2005 .

[14]  Toshifumi Takeuchi,et al.  Molecular Imprinting: From Fundamentals to Applications , 2003 .

[15]  Christian Zwiener,et al.  Oxidative treatment of pharmaceuticals in water , 2000 .

[16]  Alden K Henderson,et al.  Persistence of pharmaceutical compounds and other organic wastewater contaminants in a conventional drinking-water-treatment plant. , 2004, The Science of the total environment.

[17]  Oliver A.H. Jones,et al.  Pharmaceuticals: a threat to drinking water? , 2005, Trends in biotechnology.

[18]  M. Petrovíc.  Analysis and removal of emerging contaminants in wastewater and drinking water , 2003 .

[19]  John L. Zhou,et al.  Removal of estrone and 17β-estradiol from water by adsorption , 2005 .

[20]  Thomas Braunbeck,et al.  Combined in situ and in vitro assessment of the estrogenic activity of sewage and surface water samples. , 2003, Toxicological sciences : an official journal of the Society of Toxicology.

[21]  J. Falla,et al.  Estrogenic effects in crucian carp (Carassius carassius) exposed to treated sewage effluent. , 2005, Ecotoxicology and environmental safety.

[22]  Peter Wessels,et al.  Direct nanofiltration or ultrafiltration of WWTP effluent , 2000 .

[23]  M. Servos,et al.  Behavior and occurrence of estrogens in municipal sewage treatment plants--I. Investigations in Germany, Canada and Brazil. , 1999, The Science of the total environment.

[24]  F. Nagai,et al.  Degradation of Estrogens by Rhodococcus zopfii and Rhodococcus equi Isolates from Activated Sludge in Wastewater Treatment Plants , 2004, Applied and Environmental Microbiology.

[25]  John N. Lester,et al.  Endocrine disrupters in wastewater and sludge treatment processes , 2002 .

[26]  B. Mattiasson,et al.  Macroporous molecularly imprinted polymer/cryogel composite systems for the removal of endocrine disrupting trace contaminants. , 2007, Journal of chromatography. A.

[27]  Martin Kampmann,et al.  Ozonation: a tool for removal of pharmaceuticals, contrast media and musk fragrances from wastewater? , 2003, Water research.

[28]  E. Teller,et al.  ADSORPTION OF GASES IN MULTIMOLECULAR LAYERS , 1938 .

[29]  T. Motomura,et al.  Novosphingobium tardaugens sp. nov., an oestradiol-degrading bacterium isolated from activated sludge of a sewage treatment plant in Tokyo. , 2003, International journal of systematic and evolutionary microbiology.

[30]  C. Daughton.  Environmental stewardship and drugs as pollutants , 2002, The Lancet.

[31]  E. Thurman,et al.  Pharmaceuticals, hormones, and other organic wastewater contaminants in U.S. streams, 1999-2000: a national reconnaissance. , 2002 .

[32]  S. Weber,et al.  Degradation of estradiol and ethinyl estradiol by activated sludge and by a defined mixed culture , 2005, Applied Microbiology and Biotechnology.

[33]  H. D. Stensel,et al.  Wastewater Engineering: Treatment and Reuse , 2002 .

[34]  Y. Lévi,et al.  Assessment of river contamination by estrogenic compounds in Paris area (France). , 2004, The Science of the total environment.

[35]  C. Portier,et al.  Evaluation of chemicals with endocrine modulating activity in a yeast-based steroid hormone receptor gene transcription assay. , 1997, Toxicology and applied pharmacology.

[36]  R. Kookana,et al.  Occurrence and fate of hormone steroids in the environment. , 2002, Environment international.

[37]  S. Iwasaki,et al.  Adsorbability of estrone and 17β-estradiol in water onto activated carbon , 2006 .

[38]  H. Takada,et al.  Pharmaceutical chemicals and endocrine disrupters in municipal wastewater in Tokyo and their removal during activated sludge treatment. , 2006, Water research.