DISCO synchrotron-radiation circular-dichroism endstation at SOLEIL.

The new synchrotron-radiation circular-dichroism (SRCD) endstation on the UV-visible synchrotron beamline DISCO has been commissioned at the SOLEIL synchrotron. The design has been focused on preservation of a high degree of linear polarization at high flux and moderate resolving power covering the vacuum ultraviolet to visible spectral range (125-600 nm). The beam dimensions have been set to 4 mm × 4 mm at 1 nm bandwidth for lower sample degradation. The nitrogen-purged sample chamber fits three types of sample holders accommodating conventional round cell mounting, automated rotation of the samples, as well as a microfluidic set-up. Automated temperature-controlled data collection on microvolumes is now available to the biology and chemistry communities. Macromolecules including membrane proteins, soluble proteins, bio-nanotubes, sugars, DNA and RNAs are now routinely investigated.

[1]  M. Réfrégiers,et al.  Atmospheric pressure photoionization using tunable VUV synchrotron radiation , 2012 .

[2]  Y. Baraud,et al.  Anisotropy spectra of amino acids. , 2012, Angewandte Chemie.

[3]  R. W. Janes,et al.  Synchrotron radiation circular dichroism (SRCD) spectroscopy: An emerging method in structural biology for examining protein conformations and protein interactions , 2011 .

[4]  Y. Gohon,et al.  High water solubility and fold in amphipols of proteins with large hydrophobic regions: oleosins and caleosin from seed lipid bodies. , 2011, Biochimica et biophysica acta.

[5]  Frank Wien,et al.  International comparability in spectroscopic measurements of protein structure by circular dichroism: CCQM-P59 , 2010 .

[6]  S. Chiang,et al.  Design and construction of a compact end-station at NSRRC for circular-dichroism spectra in the vacuum-ultraviolet region. , 2010, Journal of synchrotron radiation.

[7]  Frank Wien,et al.  Synchrotron UV Fluorescence Microscopy Uncovers New Probes in Cells and Tissues , 2010, Microscopy and Microanalysis.

[8]  Yannick Sourigues,et al.  Structure and Assembly Properties of the N-Terminal Domain of the Prion Ure2p in Isolation and in Its Natural Context , 2010, PLoS ONE.

[9]  T. Jávorfi,et al.  Measuring circular dichroism in a capillary cell using the b23 synchrotron radiation CD beamline at diamond light source. , 2010, Chirality.

[10]  Daniel Zerbib,et al.  DISCO: a low-energy multipurpose beamline at synchrotron SOLEIL. , 2009, Journal of synchrotron radiation.

[11]  B. Wallace.  Protein characterisation by synchrotron radiation circular dichroism spectroscopy , 2009, Quarterly Reviews of Biophysics.

[12]  A. Ulrich,et al.  Conformation and membrane orientation of amphiphilic helical peptides by oriented circular dichroism. , 2008, Biophysical journal.

[13]  Benjamin M. Bulheller,et al.  Charge-transfer transitions in the vacuum-ultraviolet of protein circular dichroism spectra. , 2008, The journal of physical chemistry. B.

[14]  Evaluating protein:protein complex formation using synchrotron radiation circular dichroism spectroscopy , 2007, Proteins.

[15]  Jianhua Xu,et al.  High Photon Flux Beamline 4B8 for Vacuum Ultraviolet Spectroscopy at BSRF , 2007 .

[16]  R. Seckler,et al.  Mapping protein collapse with single-molecule fluorescence and kinetic synchrotron radiation circular dichroism spectroscopy , 2006, Proceedings of the National Academy of Sciences.

[17]  B A Wallace,et al.  Calcium Fluoride Micro Cells for Synchrotron Radiation Circular Dichroism Spectroscopy , 2005, Applied spectroscopy.

[18]  N. C. Price,et al.  How to study proteins by circular dichroism. , 2005, Biochimica et biophysica acta.

[19]  A. Gilles,et al.  New synchrotron radiation circular dichroism end-station on DISCO beamline at SOLEIL synchrotron for biomolecular analysis. , 2005, Biochimica et biophysica acta.

[20]  K. Gekko,et al.  Vacuum-ultraviolet circular dichroism spectrophotometer using synchrotron radiation , 2005 .

[21]  D. Svergun,et al.  Synergistic use of synchrotron radiation techniques for biological samples in solution: a case study on protein-ligand recognition by the peroxisomal import receptor Pex5p. , 2004, Journal of synchrotron radiation.

[22]  D. Clarke,et al.  CD12: a new high-flux beamline for ultraviolet and vacuum-ultraviolet circular dichroism on the SRS, Daresbury. , 2004, Journal of synchrotron radiation.

[23]  K. Gekko,et al.  Vacuum-ultraviolet circular dichroism study of saccharides by synchrotron radiation spectrophotometry. , 2004, Carbohydrate research.

[24]  T. A. Callcott,et al.  Variable groove spaced grating monochromators for synchrotron light sources , 1994 .

[25]  R. Wayne,et al.  Photochemistry of small molecules , 1979, Nature.

[26]  W. R. Hunter,et al.  The temperature dependence of the short wavelength transmittance limit of vacuum ultraviolet window materials—I. Experiment , 1969 .