Copyright © 1998, American Society for Microbiology Expression of �-Defensin Genes in Bovine Alveolar Macrophages

ABSTRACT Bovine alveolar macrophages (BAM) were examined for the expression of β-defensins and to determine whether their expression could be upregulated by bacterial lipopolysaccharide (LPS), as observed with β-defensins expressed in bovine tracheal epithelial cells. Four β-defensins were expressed constitutively in BAM, with bovine neutrophil β-defensin (BNBD)-4 and BNBD-5 being the most predominant. This is the first evidence of β-defensin gene expression in a mature myeloid cell. LPS had no effect on β-defensin expression in BAM, even though tumor necrosis factor alpha (TNF-α) production was induced. Nonbacterial inflammatory particles had little effect on β-defensin gene expression or TNF-α production in BAM. We hypothesize that constitutively expressed β-defensins of alveolar macrophages may have a role in lung host defense.

[1]  Douglas E. Jones,et al.  Enteric β-Defensin: Molecular Cloning and Characterization of a Gene with Inducible Intestinal Epithelial Cell Expression Associated with Cryptosporidium parvumInfection , 1998, Infection and Immunity.

[2]  D. Clark,et al.  Enteric beta-defensin: molecular cloning and characterization of a gene with inducible intestinal epithelial cell expression associated with Cryptosporidium parvum infection. , 1998, Infection and immunity.

[3]  J. Schröder,et al.  A peptide antibiotic from human skin , 1997, Nature.

[4]  D. Costa,et al.  Soluble transition metals mediate residual oil fly ash induced acute lung injury. , 1997, Journal of toxicology and environmental health.

[5]  James M. Wilson,et al.  Human β-Defensin-1 Is a Salt-Sensitive Antibiotic in Lung That Is Inactivated in Cystic Fibrosis , 1997, Cell.

[6]  R. Devlin,et al.  Stimulation of human and rat alveolar macrophages by urban air particulates: effects on oxidant radical generation and cytokine production. , 1996, Toxicology and applied pharmacology.

[7]  Wayne L. Smith,et al.  Purification, primary structures, and antibacterial activities of β-defensins, a new family of antimicrobial peptides from bovine neutrophils. , 1996, The Journal of Biological Chemistry.

[8]  G. Diamond,et al.  Inducible expression of an antibiotic peptide gene in lipopolysaccharide-challenged tracheal epithelial cells. , 1996, Proceedings of the National Academy of Sciences of the United States of America.

[9]  T. Scanlin,et al.  Coordinate induction of two antibiotic genes in tracheal epithelial cells exposed to the inflammatory mediators lipopolysaccharide and tumor necrosis factor alpha , 1996, Infection and immunity.

[10]  E. Greenberg,et al.  Cystic Fibrosis Airway Epithelia Fail to Kill Bacteria Because of Abnormal Airway Surface Fluid , 1996, Cell.

[11]  I. Lemaire,et al.  Distinctive profile of alveolar macrophage-derived cytokine release induced by fibrogenic and nonfibrogenic mineral dusts. , 1996, Journal of toxicology and environmental health.

[12]  A. Seidel,et al.  Influence of Coexposure of Ozone with Quartz, Latex, Albumin, and LPS on TNF-α and Chemotactic Factor Release by Bovine Alveolar Macrophages in Vitro , 1996 .

[13]  K. Wrobel,et al.  Activation of murine macrophages by silica particles in vitro is a process independent of silica-induced cell death. , 1995, American journal of respiratory cell and molecular biology.

[14]  B. Schonwetter,et al.  Epithelial antibiotics induced at sites of inflammation. , 1995, Science.

[15]  B. Mossman,et al.  Asbestos induces nuclear factor kappa B (NF-kappa B) DNA-binding activity and NF-kappa B-dependent gene expression in tracheal epithelial cells. , 1995, Proceedings of the National Academy of Sciences of the United States of America.

[16]  T. Ganz,et al.  Defensins and other endogenous peptide antibiotics of vertebrates , 1995, Journal of leukocyte biology.

[17]  M. Luster,et al.  Iron and reactive oxygen species in the asbestos-induced tumor necrosis factor-alpha response from alveolar macrophages. , 1995, American journal of respiratory cell and molecular biology.

[18]  H. Yoo,et al.  Induction of inflammatory cytokines in bovine alveolar macrophages following stimulation with Pasteurella haemolytica lipopolysaccharide , 1995, Infection and immunity.

[19]  G. Hunninghake,et al.  Silica increases tumor necrosis factor (TNF) production, in part, by upregulating the TNF promoter. , 1994, Experimental lung research.

[20]  Douglas E. Jones,et al.  Airway epithelial cells are the site of expression of a mammalian antimicrobial peptide gene. , 1993, Proceedings of the National Academy of Sciences of the United States of America.

[21]  M. Selsted,et al.  Purification, primary structures, and antibacterial activities of beta-defensins, a new family of antimicrobial peptides from bovine neutrophils. , 1993, The Journal of biological chemistry.

[22]  T. Ganz,et al.  Activity of defensins from human neutrophilic granulocytes against Mycobacterium avium-Mycobacterium intracellulare , 1992, Infection and immunity.

[23]  J. Armstrong,et al.  Type I interferons (IFN‐α and ‐β) suppress cytotoxin (tumor necrosis factor‐α and lymphotoxin) production by mitogen‐stimulated human peripheral blood mononuclear cells , 1992 .

[24]  Kevin E. Driscoll,et al.  Cytokine and Growth Factor Release by Alveolar Macrophages: Potential Biomarkers of Pulmonary Toxicity 1 , 1991, Toxicologic pathology.

[25]  M. Zasloff,et al.  Tracheal antimicrobial peptide, a cysteine-rich peptide from mammalian tracheal mucosa: peptide isolation and cloning of a cDNA. , 1991, Proceedings of the National Academy of Sciences of the United States of America.

[26]  J. Adams,et al.  Bacterial Lipopolysaccharide Induces Release of Tumor Necrosis Factor-a from Bovine Peripheral Blood Monocytes and Alveolar Macrophages in Vitro , 2022 .

[27]  C. Dubois,et al.  Asbestos fibers and silica particles stimulate rat alveolar macrophages to release tumor necrosis factor. Autoregulatory role of leukotriene B4. , 1989, The American review of respiratory disease.

[28]  M. Frohman,et al.  Rapid production of full-length cDNAs from rare transcripts: amplification using a single gene-specific oligonucleotide primer. , 1988, Proceedings of the National Academy of Sciences of the United States of America.

[29]  T. Espevik,et al.  A highly sensitive cell line, WEHI 164 clone 13, for measuring cytotoxic factor/tumor necrosis factor from human monocytes. , 1986, Journal of immunological methods.

[30]  J. Gamble,et al.  Recombinant human granulocyte-macrophage colony-stimulating factor stimulates in vitro mature human neutrophil and eosinophil function, surface receptor expression, and survival. , 1986, The Journal of clinical investigation.

[31]  J. Lewtas,et al.  Inhalable particles and pulmonary host defense: in vivo and in vitro effects of ambient air and combustion particles. , 1985, Environmental research.

[32]  D. Gardner,et al.  Stimulation of oxidant production in alveolar macrophages by pollutant and latex particles. , 1980, Environmental research.

[33]  Douglas E. Jones,et al.  Enteric β-Defensin: Molecular Cloning and Characterization of a Gene with Inducible Intestinal Epithelial Cell Expression Associated with Cryptosporidium parvumInfection , 1998, Infection and Immunity.

[34]  D. Clark,et al.  Enteric beta-defensin: molecular cloning and characterization of a gene with inducible intestinal epithelial cell expression associated with Cryptosporidium parvum infection. , 1998, Infection and immunity.

[35]  J. Schröder,et al.  A peptide antibiotic from human skin , 1997, Nature.

[36]  D. Costa,et al.  Soluble transition metals mediate residual oil fly ash induced acute lung injury. , 1997, Journal of toxicology and environmental health.

[37]  James M. Wilson,et al.  Human β-Defensin-1 Is a Salt-Sensitive Antibiotic in Lung That Is Inactivated in Cystic Fibrosis , 1997, Cell.

[38]  R. Devlin,et al.  Stimulation of human and rat alveolar macrophages by urban air particulates: effects on oxidant radical generation and cytokine production. , 1996, Toxicology and applied pharmacology.

[39]  Wayne L. Smith,et al.  Purification, primary structures, and antibacterial activities of β-defensins, a new family of antimicrobial peptides from bovine neutrophils. , 1996, The Journal of Biological Chemistry.

[40]  G. Diamond,et al.  Inducible expression of an antibiotic peptide gene in lipopolysaccharide-challenged tracheal epithelial cells. , 1996, Proceedings of the National Academy of Sciences of the United States of America.

[41]  T. Scanlin,et al.  Coordinate induction of two antibiotic genes in tracheal epithelial cells exposed to the inflammatory mediators lipopolysaccharide and tumor necrosis factor alpha , 1996, Infection and immunity.

[42]  E. Greenberg,et al.  Cystic Fibrosis Airway Epithelia Fail to Kill Bacteria Because of Abnormal Airway Surface Fluid , 1996, Cell.

[43]  I. Lemaire,et al.  Distinctive profile of alveolar macrophage-derived cytokine release induced by fibrogenic and nonfibrogenic mineral dusts. , 1996, Journal of toxicology and environmental health.

[44]  A. Seidel,et al.  Influence of Coexposure of Ozone with Quartz, Latex, Albumin, and LPS on TNF-α and Chemotactic Factor Release by Bovine Alveolar Macrophages in Vitro , 1996 .

[45]  K. Wrobel,et al.  Activation of murine macrophages by silica particles in vitro is a process independent of silica-induced cell death. , 1995, American journal of respiratory cell and molecular biology.

[46]  B. Mossman,et al.  Asbestos induces nuclear factor kappa B (NF-kappa B) DNA-binding activity and NF-kappa B-dependent gene expression in tracheal epithelial cells. , 1995, Proceedings of the National Academy of Sciences of the United States of America.

[47]  T. Ganz,et al.  Defensins and other endogenous peptide antibiotics of vertebrates , 1995, Journal of leukocyte biology.

[48]  M. Luster,et al.  Iron and reactive oxygen species in the asbestos-induced tumor necrosis factor-alpha response from alveolar macrophages. , 1995, American journal of respiratory cell and molecular biology.

[49]  B. Schonwetter,et al.  Epithelial antibiotics induced at sites of inflammation , 1995, Science.

[50]  H. Yoo,et al.  Induction of inflammatory cytokines in bovine alveolar macrophages following stimulation with Pasteurella haemolytica lipopolysaccharide , 1995, Infection and immunity.

[51]  G. Hunninghake,et al.  Silica increases tumor necrosis factor (TNF) production, in part, by upregulating the TNF promoter. , 1994, Experimental lung research.

[52]  Douglas E. Jones,et al.  Airway epithelial cells are the site of expression of a mammalian antimicrobial peptide gene. , 1993, Proceedings of the National Academy of Sciences of the United States of America.

[53]  T. Ganz,et al.  Activity of defensins from human neutrophilic granulocytes against Mycobacterium avium-Mycobacterium intracellulare , 1992, Infection and immunity.

[54]  J. Armstrong,et al.  Type I interferons (IFN‐α and ‐β) suppress cytotoxin (tumor necrosis factor‐α and lymphotoxin) production by mitogen‐stimulated human peripheral blood mononuclear cells , 1992 .

[55]  Kevin E. Driscoll,et al.  Cytokine and Growth Factor Release by Alveolar Macrophages: Potential Biomarkers of Pulmonary Toxicity 1 , 1991, Toxicologic pathology.

[56]  M. Zasloff,et al.  Tracheal antimicrobial peptide, a cysteine-rich peptide from mammalian tracheal mucosa: peptide isolation and cloning of a cDNA. , 1991, Proceedings of the National Academy of Sciences of the United States of America.

[57]  J. Adams,et al.  Bacterial Lipopolysaccharide Induces Release of Tumor Necrosis Factor-a from Bovine Peripheral Blood Monocytes and Alveolar Macrophages in Vitro , 2022 .

[58]  C. Dubois,et al.  Asbestos fibers and silica particles stimulate rat alveolar macrophages to release tumor necrosis factor. Autoregulatory role of leukotriene B4. , 1989, The American review of respiratory disease.

[59]  M. Frohman,et al.  Rapid production of full-length cDNAs from rare transcripts: amplification using a single gene-specific oligonucleotide primer. , 1988, Proceedings of the National Academy of Sciences of the United States of America.

[60]  T. Espevik,et al.  A highly sensitive cell line, WEHI 164 clone 13, for measuring cytotoxic factor/tumor necrosis factor from human monocytes. , 1986, Journal of immunological methods.

[61]  J. Gamble,et al.  Recombinant human granulocyte-macrophage colony-stimulating factor stimulates in vitro mature human neutrophil and eosinophil function, surface receptor expression, and survival. , 1986, The Journal of clinical investigation.

[62]  J. Lewtas,et al.  Inhalable particles and pulmonary host defense: in vivo and in vitro effects of ambient air and combustion particles. , 1985, Environmental research.

[63]  D. Gardner,et al.  Stimulation of oxidant production in alveolar macrophages by pollutant and latex particles. , 1980, Environmental research.