Immobilization of enzymes on a microchannel surface through cross-linking polymerization.

A novel and facile method for the preparation of an enzyme-immobilized microreactor has been developed in which enzymes are immobilized as an enzyme-polymer membrane formed on the inner wall of the microchannel by a cross-linking polymerization method; the resulting microreactor shows excellent reaction performance and stability against denaturating agents.

[1]  H. Maeda,et al.  Reevaluation of the reaction of formaldehyde at low concentration with amino acids. , 1980, Journal of biochemistry.

[2]  Paul Watts,et al.  Micro reactors: principles and applications in organic synthesis , 2002 .

[3]  J. Khandurina,et al.  Bioanalysis in microfluidic devices. , 2002, Journal of chromatography. A.

[4]  Pierre Monsan,et al.  Étude du mécanisme d'établissement des liaisons glutaraldéhyde-protéines , 1976 .

[5]  M. Uehara,et al.  Modified micro-space using self-organized nanoparticles for reduction of methylene blue. , 2003, Chemical communications.

[6]  M. Morra,et al.  Surface field of forces and protein adsorption behavior of poly(hydroxyethylmethacrylate) films deposited from plasma. , 1995, Journal of biomedical materials research.

[7]  Tibor Chován,et al.  Microfabricated devices in biotechnology and biochemical processing. , 2002, Trends in biotechnology.

[8]  T. Rohr,et al.  High-throughput peptide mass mapping using a microdevice containing trypsin immobilized on a porous polymer monolith coupled to MALDI TOF and ESI TOF mass spectrometers. , 2002, Journal of proteome research.

[9]  Frédéric Reymond,et al.  Why the move to microfluidics for protein analysis? , 2004, Current opinion in biotechnology.

[10]  S. Noinville,et al.  Conformational changes of enzymes adsorbed at liquid-solid interface: relevance to enzymatic activity. , 2002, Biopolymers.

[11]  Hideaki Maeda,et al.  Simple method for preparation of nanostructure on microchannel surface and its usage for enzyme-immobilization. , 2003, Chemical communications.

[12]  Hideaki Maeda,et al.  Preparation of functionalized nanostructures on microchannel surface and their use for enzyme microreactors , 2004 .

[13]  Hiroyuki Nakamura,et al.  Efficient immobilization of enzymes on microchannel surface through His-tag and application for microreactor. , 2005, Protein and peptide letters.

[14]  M. Kirschner,et al.  Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis. , 1977, The Journal of biological chemistry.

[15]  M. Navia,et al.  Cross-linked enzyme crystals as robust biocatalysts , 1992 .

[16]  M. Novotny,et al.  Analytical characterization of a facile porous polymer monolithic trypsin microreactor enabling peptide mass mapping using mass spectrometry. , 2004, Rapid communications in mass spectrometry : RCM.

[17]  Volker Hessel,et al.  High Throughput Kinetic Investigations of Asymmetric Hydrogenations with Microdevices , 2003 .

[18]  Hideaki Maeda,et al.  A simple method for surface modification of microchannels , 2003 .