The riboswitch-mediated control of sulfur metabolism in bacteria

Many operons in Gram-positive bacteria that are involved in methionine (Met) and cysteine (Cys) biosynthesis possess an evolutionarily conserved regulatory leader sequence (S-box) that positively controls these genes in response to methionine starvation. Here, we demonstrate that a feed-back regulation mechanism utilizes S-adenosyl-methionine as an effector. S-adenosyl-methionine directly and specifically binds to the nascent S-box RNA, causing an intrinsic terminator to form and interrupt transcription prematurely. The S-box leader RNA thus expands the family of newly discovered riboswitches, i.e., natural regulatory RNA aptamers that seem to sense small molecules ranging from amino acid derivatives to vitamins.

[1]  Ali Nahvi,et al.  Genetic control by a metabolite binding mRNA. , 2002, Chemistry & biology.

[2]  G. Storz,et al.  Take your vitamins with a pinch of RNA. , 2002, Molecular cell.

[3]  A. Danchin,et al.  The metIC operon involved in methionine biosynthesis in Bacillus subtilis is controlled by transcription antitermination. , 2002, Microbiology.

[4]  R. Kadner,et al.  Adenosylcobalamin inhibits ribosome binding to btuB RNA. , 2000, Proceedings of the National Academy of Sciences of the United States of America.

[5]  T. Henkin,et al.  Prediction of Gene Function in Methylthioadenosine Recycling from Regulatory Signals , 2002, Journal of bacteriology.

[6]  Evgeny Nudler,et al.  Sensing Small Molecules by Nascent RNA A Mechanism to Control Transcription in Bacteria , 2002, Cell.

[7]  Jan Barciszewski,et al.  RNA Biochemistry and Biotechnology , 1999 .

[8]  T. Henkin,et al.  The T box and S box transcription termination control systems. , 2003, Frontiers in bioscience : a journal and virtual library.

[9]  T. Henkin,et al.  The S box regulon: a new global transcription termination control system for methionine and cysteine biosynthesis genes in Gram‐positive bacteria , 1998, Molecular microbiology.

[10]  J. Miranda-Ríos,et al.  A conserved RNA structure (thi box) is involved in regulation of thiamin biosynthetic gene expression in bacteria , 2001, Proceedings of the National Academy of Sciences of the United States of America.

[11]  C. Yanofsky,et al.  Regulation by transcription attenuation in bacteria: how RNA provides instructions for transcription termination/antitermination decisions. , 2002, BioEssays : news and reviews in molecular, cellular and developmental biology.

[12]  M. Gelfand,et al.  A conserved RNA structure element involved in the regulation of bacterial riboflavin synthesis genes. , 1999, Trends in genetics : TIG.

[13]  I. H. Öğüş,et al.  NATO ASI Series , 1997 .

[14]  R. Burgess,et al.  Rapid purification of His(6)-tagged Bacillus subtilis core RNA polymerase. , 2000, Protein expression and purification.

[15]  A. Danchin,et al.  Global Expression Profile of Bacillus subtilis Grown in the Presence of Sulfate or Methionine , 2002, Journal of bacteriology.

[16]  E. Nudler,et al.  The mechanism of intrinsic transcription termination. , 1999, Molecular cell.

[17]  Ronald R. Breaker,et al.  Thiamine derivatives bind messenger RNAs directly to regulate bacterial gene expression , 2002, Nature.

[18]  G N Cohen,et al.  Interactions of the Escherichia coli methionine repressor with the metF operator and with its corepressor, S-adenosylmethionine. , 1986, The Journal of biological chemistry.

[19]  Cloning and characterization of the metE gene encoding S-adenosylmethionine synthetase from Bacillus subtilis , 1996, Journal of bacteriology.

[20]  M. Gelfand,et al.  Comparative Genomics of Thiamin Biosynthesis in Procaryotes , 2002, The Journal of Biological Chemistry.

[21]  M. Gelfand,et al.  Regulation of riboflavin biosynthesis and transport genes in bacteria by transcriptional and translational attenuation. , 2002, Nucleic acids research.