STUDIES ON THE LIGHT TRANSMISSION OF NONHEMOLYZED WHOLE BLOOD. DETERMINATION OF OXYGEN SATURATION.

Abstract Previously, calibration of whole blood oximeters required that an empirical relationship be established between the manometric determinations of oxygen saturation and oximeter readings. Although measurements of oxygen saturation, using these techniques were adequate for routine clinical use, they showed considerable inaccuracy at low hemoglobin concentrations. The method described presents a new approach to calibration. Mathematical expressions were derived relating light transmission of flowing nonhemolyzed whole blood to oxygen saturation and hemoglobin concentration. Thus, the need for empirical calibration against reference methods is eliminated. Thirty-two samples ranging in hemoglobin concentration from 5.4 to 21.4 Gm. per 100 ml., and in saturation from 34.9 to 100 per cent were analyzed. The standard deviation of the differences between oximetric determinations and Van Slyke analyses were ± 1.07 per cent and ± 1.36 per cent, depending on whether the measurements were made with a one wavelength or two wavelength system. The results show a significant increase in accuracy, particularly at low hemoglobin concentrations where previous techniques were highly unreliable.

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