An active supercomplex of NADPH dehydrogenase mediated cyclic electron flow around Photosystem I from the panicle chloroplast of Oryza sativa.

Chloroplast NAD(P)H dehydrogenase-like complex (NDH) plays a crucial role in the protection of plants against oxidative stress. In higher plants, NDH interacts with Photosystem I (PSI) to form an NDH-PSI supercomplex. However, the chloroplast supercomplex with NADPH oxidation activity remains to be identified. Here, we reported the identification of a supercomplex of NDH with NADPH-nitroblue tetrazolium oxidoreductase activity in the chloroplast of rice panicle. The active supercomplex from the panicle chloroplast contained higher amounts of the NDH subunits (NdhH, NdhK, and NdhA) than that from the flag leaf chloroplast. The highly active supercomplex might underlie the high activity of the NADPH-dependent NDH pathway and the larger proton gradient across thylakoid membranes via cyclic electron flow around PSI, as well as the higher maximal photochemical efficiency of Photosystem II at the flowering to grain-filling stage. The supercomplex is suggested to be essential for the high efficiency of photosynthesis and play a protective role in the grain formation in rice plant.

[1]  T. Shikanai,et al.  In Planta Mutagenesis of Src Homology 3 Domain-like Fold of NdhS, a Ferredoxin-binding Subunit of the Chloroplast NADH Dehydrogenase-like Complex in Arabidopsis , 2013, The Journal of Biological Chemistry.

[2]  Peng Hu,et al.  Enzymatic characterization of an active NDH complex from Thermosynechococcus elongatus , 2013, FEBS letters.

[3]  John M. Berrisford,et al.  Crystal structure of the entire respiratory complex I , 2013, Nature.

[4]  G. Sanguinetti,et al.  Systems analysis of transcription factor activities in environments with stable and dynamic oxygen concentrations , 2012, Open Biology.

[5]  Weimin Ma,et al.  Regulation of NAD(P)H dehydrogenase-dependent cyclic electron transport around PSI by NaHSO₃ at low concentrations in tobacco chloroplasts. , 2011, Plant & cell physiology.

[6]  T. Shikanai,et al.  Structure of the chloroplast NADH dehydrogenase-like complex: nomenclature for nuclear-encoded subunits. , 2011, Plant & cell physiology.

[7]  E. Aro,et al.  Identification of Novel Ssl0352 Protein (NdhS), Essential for Efficient Operation of Cyclic Electron Transport around Photosystem I, in NADPH:plastoquinone Oxidoreductase (NDH-1) Complexes of Synechocystis sp. PCC 6803* , 2011, The Journal of Biological Chemistry.

[8]  E. Aro,et al.  Cyanobacterial NDH-1 complexes: novel insights and remaining puzzles. , 2011, Biochimica et biophysica acta.

[9]  T. Shikanai,et al.  An Src Homology 3 Domain-Like Fold Protein Forms a Ferredoxin Binding Site for the Chloroplast NADH Dehydrogenase-Like Complex in Arabidopsis[W] , 2011, Plant Cell.

[10]  T. Shikanai,et al.  Supercomplex Formation with Photosystem I Is Required for the Stabilization of the Chloroplast NADH Dehydrogenase-Like Complex in Arabidopsis1[W] , 2011, Plant Physiology.

[11]  Yoichiro Fukao,et al.  Efficient Operation of NAD(P)H Dehydrogenase Requires Supercomplex Formation with Photosystem I via Minor LHCI in Arabidopsis[W] , 2009, The Plant Cell Online.

[12]  Mao Weimin,et al.  Dynamic Changes of Photosynthetic Characteristics in Xiaoyan 54, Jing 411, and the Stable Selected Superior Strains of Their Hybrid Progenies: Dynamic Changes of Photosynthetic Characteristics in Xiaoyan 54, Jing 411, and the Stable Selected Superior Strains of Their Hybrid Progenies , 2009 .

[13]  Cheng Jian.  Dynamic Changes of Photosynthetic Characteristics in Xiaoyan 54,Jing 411,and the Stable Selected Superior Strains of Their Hybrid Progenies , 2009 .

[14]  T. Shikanai,et al.  The Chloroplast NAD(P)H Dehydrogenase Complex Interacts with Photosystem I in Arabidopsis* , 2008, Journal of Biological Chemistry.

[15]  Weimin Ma,et al.  Effect of exogenous glucose on the expression and activity of NADPH dehydrogenase complexes in the cyanobacterium Synechocystis sp. strain PCC 6803. , 2008, Plant physiology and biochemistry : PPB.

[16]  S. Masiero,et al.  A Complex Containing PGRL1 and PGR5 Is Involved in the Switch between Linear and Cyclic Electron Flow in Arabidopsis , 2008, Cell.

[17]  T. Shikanai,et al.  Regulation of Photosynthesis via PSI Cyclic Electron Transport , 2008 .

[18]  T. Ogawa,et al.  Active NDH-1 complexes from the cyanobacterium Synechocystis sp. strain PCC 6803. , 2006, Plant & cell physiology.

[19]  H. Mi,et al.  The role of chloroplast NAD(P)H dehydrogenase in protection of tobacco plant against heat stress , 2006, Science in China Series C: Life Sciences.

[20]  T. Shikanai,et al.  Chloroplastic NAD(P)H Dehydrogenase in Tobacco Leaves Functions in Alleviation of Oxidative Damage Caused by Temperature Stress1[OA] , 2006, Plant Physiology.

[21]  Costel C. Darie,et al.  Isolation and structural characterization of the Ndh complex from mesophyll and bundle sheath chloroplasts of Zea mays , 2005, The FEBS journal.

[22]  J. Garin,et al.  New Subunits NDH-M, -N, and -O, Encoded by Nuclear Genes, Are Essential for Plastid Ndh Complex Functioning in Higher Plantsw⃞ , 2005, The Plant Cell Online.

[23]  U. Heber,et al.  Rates of vectorial proton transport supported by cyclic electron flow during oxygen reduction by illuminated intact chloroplasts , 1994, Photosynthesis Research.

[24]  T. Hibino,et al.  Photo-induction of an NADPH dehydrogenase which functions as a mediator of electron transport to the intersystem chain in the cyanobacterium Synechocystis PCC6803 , 2004, Photosynthesis Research.

[25]  Yunkang Shen.  Dynamic approaches to the mechanism of photosynthesis , 2004, Photosynthesis Research.

[26]  W. Bilger,et al.  Continuous recording of photochemical and non-photochemical chlorophyll fluorescence quenching with a new type of modulation fluorometer , 2004, Photosynthesis Research.

[27]  H. Mi,et al.  [Separation of hydrophobic NAD(P)H dehydrogenase subcomplexes from cyanobacterium Synechocystis PCC6803]. , 2003, Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica.

[28]  H. Mi,et al.  Effects of low CO2 on NAD(P)H dehydrogenase, a mediator of cyclic electron transport around photosystem I in the cyanobacterium synechocystis PCC6803. , 2003, Plant & cell physiology.

[29]  J. Meurer,et al.  PGR5 Is Involved in Cyclic Electron Flow around Photosystem I and Is Essential for Photoprotection in Arabidopsis , 2002, Cell.

[30]  U. Schreiber,et al.  Light-induced dynamic changes of NADPH fluorescence in Synechocystis PCC 6803 and its ndhB-defective mutant M55. , 2000, Plant & cell physiology.

[31]  T. Friedrich,et al.  The respiratory complex I of bacteria, archaea and eukarya and its module common with membrane‐bound multisubunit hydrogenases , 2000, FEBS letters.

[32]  G. Peltier,et al.  Targeted inactivation of the plastid ndhB gene in tobacco results in an enhanced sensitivity of photosynthesis to moderate stomatal closure. , 2000, Plant physiology.

[33]  F. Sato,et al.  The role of chloroplastic NAD(P)H dehydrogenase in photoprotection , 1999, FEBS letters.

[34]  Yasuyuki Yamada,et al.  Directed disruption of the tobacco ndhB gene impairs cyclic electron flow around photosystem I. , 1998, Proceedings of the National Academy of Sciences of the United States of America.

[35]  H. Koop,et al.  Mutagenesis of the genes encoding subunits A, C, H, I, J and K of the plastid NAD(P)H-plastoquinone-oxidoreductase in tobacco by polyethylene glycol-mediated plastome transformation , 1998, Molecular and General Genetics MGG.

[36]  P. Nixon,et al.  Identification of a functional respiratory complex in chloroplasts through analysis of tobacco mutants containing disrupted plastid ndh genes , 1998, The EMBO journal.

[37]  U. Schreiber,et al.  Measuring P700 Absorbance Changes in the Near Infrared Spectral Region with a Dual Wavelength Pulse Modulation System , 1998 .

[38]  G. Garab,et al.  Photosynthesis: Mechanisms and Effects , 1998, Springer Netherlands.

[39]  Bernhard Teicher H,et al.  The NAD(P)H dehydrogenase in barley thylakoids is photoactivatable and uses NADPH as well as NADH , 1998, Plant physiology.

[40]  K. Asada,et al.  Thylakoid Membrane-Bound, NADPH-Specific Pyridine Nucleotide Dehydrogenase Complex Mediates Cyclic Electron Transport in the Cyanobacterium Synechocystis sp. PCC 6803 , 1995 .

[41]  D. Bendall,et al.  Cyclic photophosphorylation and electron transport , 1995 .

[42]  K. Asada,et al.  NAD(P)H Dehydrogenase-Dependent Cyclic Electron Flow around Photosystem I in the Cyanobacterium Synechocystis PCC 6803: a Study of Dark-Starved Cells and Spheroplasts , 1994 .

[43]  K. Asada,et al.  Donation of Electrons from Cytosolic Components to the Intersystem Chain in the Cyanobacterium Synechococcus sp. PCC 7002 as Determined by the Reduction of P700 , 1992 .

[44]  K. Asada,et al.  Electron donation from cyclic and respiratory flows to the photosynthetic intersystem chain is mediated by pyridine nucleotide dehydrogenase in the cyanobacterium Synechocystis PCC 6803 , 1992 .

[45]  R. J. Porra,et al.  Determination of accurate extinction coefficients and simultaneous equations for assaying chlorophylls a and b extracted with four different solvents: verification of the concentration of chlorophyll standards by atomic absorption spectroscopy , 1989 .

[46]  J. Briantais,et al.  The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence , 1989 .

[47]  G. Hind,et al.  Correlation between photosynthesis and the transthylakoid proton gradient. , 1981, Biochimica et biophysica acta.

[48]  C. Giersch,et al.  CO2 reduction by intact chloroplasts under a diminished proton gradient. , 1977, Biochimica et biophysica acta.

[49]  P. Schürmann,et al.  Role of cyclic photophosphorylation in photosynthetic carbon dioxide assimilation by isolated chloroplasts. , 1972, Biochimica et biophysica acta.

[50]  M. Avron,et al.  Determination of pH in chloroplasts. I. Distribution of ( 14 C) methylamine. , 1972, European journal of biochemistry.

[51]  U. K. Laemmli,et al.  Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4 , 1970, Nature.