Effects of lambda-phage infection on bacterial synthesis.

Abstract RNA and protein synthesis in λ infected cells have been studied. A marked depression of the synthesis of these macromolecules was observed, and was found to depend on the multiplicity of infection. This effect occurs in sensitive cells as well as in lysogenic immune cells. Depression of RNA synthesis takes place even if the cells have been pretreated with chloramphenicol and this depression seems to affect all molecular species of RNA equally. The types of newly synthesized proteins are substantially similar to those of the uninfected control. Host restricted λ or ghosts of phage λ do not show this effect, suggesting that the effects are not due to a protein component of the phage itself.

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