Two-parameter angular light scatter collection for microfluidic flow cytometry by unique waveguide structures

Waveguide numerical aperture restrictions and light-blocking elements are used to create a microfluidic cytometer with both illumination and two-parameter light scatter collection systems integrated on-chip. Good forward scatter coefficients of variation (9.7-18.3%) are achieved for polystyrene beads under a reasonably high flow rate (28 cm/s) using a greatly simplified optical system.

[1]  Luke P. Lee,et al.  Disposable integrated microfluidics with self-aligned planar microlenses , 2004 .

[2]  J. Kutter,et al.  Integrating advanced functionality in a microfabricated high-throughput fluorescent-activated cell sorter. , 2003, Lab on a chip.

[3]  V. Lien,et al.  Demonstration of two-dimensional fluidic lens for integration into microfluidic flow cytometers , 2006 .

[4]  V. Lien,et al.  Integrated Fluidic Photonics for Multi-Parameter In-Plane Detection in Microfluidic Flow Cytometry , 2006, LEOS 2006 - 19th Annual Meeting of the IEEE Lasers and Electro-Optics Society.

[5]  K. Mogensen,et al.  Measurements of scattered light on a microchip flow cytometer with integrated polymer based optical elements. , 2004, Lab on a chip.

[6]  Yu-Hwa Lo,et al.  Microfluidic cell sorter with integrated piezoelectric actuator , 2009, Biomedical microdevices.

[7]  J. Michael Ramsey,et al.  Microchip flow cytometry using electrokinetic focusing. , 1999, Analytical chemistry.

[8]  Ryuji Koyama,et al.  Counting and sizing of particles and particle agglomerates in a microfluidic device using laser light scattering: application to a particle-enhanced immunoassay. , 2003, Lab on a chip.

[9]  G. Whitesides,et al.  Rapid Prototyping of Microfluidic Systems in Poly(dimethylsiloxane). , 1998, Analytical chemistry.

[10]  Yu-Hwa Lo,et al.  Microfluidic photonic integrated circuits , 2008, SPIE/OSA/IEEE Asia Communications and Photonics.

[11]  H. Steen,et al.  Light scattering measurement in an arc lamp-based flow cytometer. , 1990, Cytometry.

[12]  A. Kummrow,et al.  Microfluidic structures for flow cytometric analysis of hydrodynamically focussed blood cells fabricated by ultraprecision micromachining. , 2009, Lab on a chip.

[13]  Lisa R. Hilliard,et al.  Multi-wavelength microflow cytometer using groove-generated sheath flow. , 2009, Lab on a chip.

[14]  V. Lien,et al.  A prealigned process of integrating optical waveguides with microfluidic devices , 2004, IEEE Photonics Technology Letters.