The goal of this article is to present a method ology for collecting and analyzing bio-signal data that represent driver's psycho-physiological response, to evaluate the influence of road condition on human subjects during highway driving. To achieve this goal, relevance of the existing analysis methods for various bio-signals was examined, and a new dataset on the bio-signals for various driving difficulty levels resulted from the road geometry was collected for seventy-three subjects driving Yongdong Highway and Seohean Highway. After detailed analyses of the collected data, the 12 important parameters were determined that could effectively measure driver's mental stress in driving. Based on those parameters, we used the Demand-Effort Model (Kim et al., 2002) as a diagnostic approach for evaluating road safety reflecting driver's bio-signal by applying Just Noticeable Difference (JND) method based on the size and slope of each signal.
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