miR‐146a interacting with lncRNA EPB41L4A‐AS1 and lncRNA SNHG7 inhibits proliferation of bone marrow‐derived mesenchymal stem cells

Emerging evidence suggests that microRNA plays a pivotal role in cell proliferation. Our previous research has certified that miR‐146a attenuates osteoarthritis through the regulation of cartilage homeostasis. However, little information about the function of miR‐146a in bone marrow‐derived mesenchymal stem cells (BMSCs) proliferation and the underlying mechanism was available. Therefore, this study aims at investigating the role of miR‐146a on the proliferation of BMSCs and the possible mechanisms involved. The function of miR‐146a on BMSCs proliferation was studied through overexpression and knockdown of miR‐146a or the indicated long noncoding RNAs (lncRNAs) in BMSCs and then the proliferation rate of the BMSCs were detected by Cell Counting Kit‐8 assay, colony formation assay. Besides, flow cytometry was used to test the cell cycle state of BMSCs modified by overexpression or knockdown of miR‐146a or lncRNA EPB41L4A‐AS1 (EPB41L4A Antisense RNA 1) and small nucleolar RNA host gene 7 (SNHG7). The expression level of marker genes involved in modulating cell proliferation was evaluated by quantitative polymerase chain reaction and western blot analysis. We discovered that the knockdown of miR‐146a significantly promoted BMSCs proliferation. Moreover, miR‐146a could bind to and inhibit endogenous expression of EPB41L4A‐AS1 and SNHG7. Further study demonstrated that overexpression of EPB41L4A‐AS1 and SNHG7 significantly enhanced proliferation of BMSCs. For the first time, we certified that miR‐146a suppressed BMSCs proliferation, but EPB41L4A‐AS1 and SNHG7 promoted BMSCs proliferation in the present study. Mechanistically, miR‐146a significantly inhibited BMSCs proliferation partly through miR‐146a/EPB41L4A‐AS1 SNHG7/cell proliferation signaling pathway axis.

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