DNA conformational analysis in solution by uranyl mediated photocleavage
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Uranyl mediated photocleavage of double stranded DNA is proposed as a general probing for DNA helix conformation in terms of minor groove width/electronegative potential. Specifically, it is found that A/T-tracts known to constitute strong distamycin binding sites are preferentially photocleaved by uranyl in a way indicating strongest uranyl binding at the center of the minor groove of the AT-region. The A-tracts of kinetoplast DNA show the highest reactivity at the 3'-end of the tract--as opposed to cleavage by EDTA/Fell--in accordance with the minor groove being more narrow at this end. Finally, uranyl photocleavage of the internal control region (ICR) of the 5S-RNA gene yields a cleavage modulation pattern fully compatible with that obtained by DNase I which also--in a more complex way--senses DNA minor groove width.
[1] E. Triphosphat,et al. FEBS Letters , 1987, FEBS Letters.
[2] J. Sambrook,et al. Molecular Cloning: A Laboratory Manual , 2001 .
[3] H. Sambrook. Molecular cloning : a laboratory manual. Cold Spring Harbor, NY , 1989 .