The road much traveled: trafficking in the cell nucleus.

Trafficking of RNA molecules and proteins within the cell nucleus is central to genome function. Recent work has revealed the nature of RNA and protein motion within the nucleus and across the nuclear membrane. These studies have given insight into how molecules find their destinations within the nucleus and have uncovered some of the structural properties of the nuclear microenvironment. Control of RNA and protein trafficking is now emerging as a physiological regulatory mechanism in gene expression and nuclear function.

[1]  Heinrich Leonhardt,et al.  DNA polymerase clamp shows little turnover at established replication sites but sequential de novo assembly at adjacent origin clusters. , 2002, Molecular cell.

[2]  G. Blobel,et al.  Gene gating: a hypothesis. , 1985, Proceedings of the National Academy of Sciences of the United States of America.

[3]  H. Tanke,et al.  Poly(A)+ RNAs roam the cell nucleus and pass through speckle domains in transcriptionally active and inactive cells , 2004, The Journal of cell biology.

[4]  P. Lichter,et al.  Histone acetylation increases chromatin accessibility , 2005, Journal of Cell Science.

[5]  Ed Hurt,et al.  Pre-ribosomes on the road from the nucleolus to the cytoplasm. , 2003, Trends in cell biology.

[6]  Sanjay Tyagi,et al.  Mechanism of mRNA transport in the nucleus. , 2005, Proceedings of the National Academy of Sciences of the United States of America.

[7]  A. Verkman,et al.  Translational Diffusion of Macromolecule-sized Solutes in Cytoplasm and Nucleus , 1997, The Journal of cell biology.

[8]  A. Lamond,et al.  Newly assembled snRNPs associate with coiled bodies before speckles, suggesting a nuclear snRNP maturation pathway , 1999, Current Biology.

[9]  T Misteli,et al.  Protein dynamics: implications for nuclear architecture and gene expression. , 2001, Science.

[10]  P. Lichter,et al.  Diffusion-limited compartmentalization of mammalian cell nuclei assessed by microinjected macromolecules. , 2003, Experimental cell research.

[11]  J. Politz,et al.  Diffusion-based transport of nascent ribosomes in the nucleus. , 2003, Molecular Biology of the Cell.

[12]  Sui Huang,et al.  Nucleolar Components Involved in Ribosome Biogenesis Cycle between the Nucleolus and Nucleoplasm in Interphase Cells , 2001, The Journal of cell biology.

[13]  Maria Carmo-Fonseca,et al.  Intracellular macromolecular mobility measured by fluorescence recovery after photobleaching with confocal laser scanning microscopes. , 2004, Molecular biology of the cell.

[14]  Tom Misteli,et al.  Maintenance of Stable Heterochromatin Domains by Dynamic HP1 Binding , 2003, Science.

[15]  R. Singer,et al.  Movement of nuclear poly(A) RNA throughout the interchromatin space in living cells , 1999, Current Biology.

[16]  Lawrence M. Lifshitz,et al.  Rapid, diffusional shuttling of poly(A) RNA between nuclear speckles and the nucleoplasm. , 2005, Molecular biology of the cell.

[17]  T. Misteli,et al.  Competition between histone H1 and HMGN proteins for chromatin binding sites , 2002, EMBO reports.

[18]  S. Halford,et al.  Measurement of the contributions of 1D and 3D pathways to the translocation of a protein along DNA. , 2005, Proceedings of the National Academy of Sciences of the United States of America.

[19]  J. Gall,et al.  Cajal bodies, nucleoli, and speckles in the Xenopus oocyte nucleus have a low-density, sponge-like structure. , 2004, Molecular biology of the cell.

[20]  Elmar Wahle,et al.  The intranuclear mobility of messenger RNA binding proteins is ATP dependent and temperature sensitive , 2002, The Journal of cell biology.

[21]  S. Diekmann,et al.  High- and low-mobility populations of HP1 in heterochromatin of mammalian cells. , 2004, Molecular biology of the cell.

[22]  T. Cremer,et al.  Chromosome territories, nuclear architecture and gene regulation in mammalian cells , 2001, Nature Reviews Genetics.

[23]  A. Verkman.  Solute and macromolecule diffusion in cellular aqueous compartments. , 2002, Trends in biochemical sciences.

[24]  Tom Misteli,et al.  Kinetic modelling approaches to in vivo imaging , 2001, Nature Reviews Molecular Cell Biology.

[25]  J. Marko,et al.  How do site-specific DNA-binding proteins find their targets? , 2004, Nucleic acids research.

[26]  J. Politz,et al.  A nonribosomal landscape in the nucleolus revealed by the stem cell protein nucleostemin. , 2005, Molecular biology of the cell.

[27]  D. Kioussis,et al.  Modulation of Heterochromatin Protein 1 Dynamics in Primary Mammalian Cells , 2003, Science.

[28]  H. Tanke,et al.  Mutational Analysis of Fibrillarin and Its Mobility in Living Human Cells , 2000, The Journal of cell biology.

[29]  R. McKay,et al.  A multistep, GTP-driven mechanism controlling the dynamic cycling of nucleostemin , 2005, The Journal of cell biology.

[30]  T. Misteli,et al.  In vivo kinetics of Cajal body components , 2004, The Journal of cell biology.

[31]  M. Kampmann.  Facilitated diffusion in chromatin lattices: mechanistic diversity and regulatory potential , 2005, Molecular microbiology.

[32]  M. Hendzel,et al.  Rapid exchange of histone H1.1 on chromatin in living human cells , 2000, Nature.

[33]  James G McNally,et al.  FRAP analysis of binding: proper and fitting. , 2005, Trends in cell biology.

[34]  A. Houtsmuller,et al.  Rapid switching of TFIIH between RNA polymerase I and II transcription and DNA repair in vivo. , 2002, Molecular cell.

[35]  J Langowski,et al.  Anomalous diffusion of fluorescent probes inside living cell nuclei investigated by spatially-resolved fluorescence correlation spectroscopy. , 2000, Journal of molecular biology.

[36]  J. Ellenberg,et al.  Mapping the dynamic organization of the nuclear pore complex inside single living cells , 2004, Nature Cell Biology.

[37]  W. Kaelin,et al.  Molecular basis of the VHL hereditary cancer syndrome , 2002, Nature Reviews Cancer.

[38]  D. E. Wolf,et al.  Intranuclear diffusion and hybridization state of oligonucleotides measured by fluorescence correlation spectroscopy in living cells. , 1998, Proceedings of the National Academy of Sciences of the United States of America.

[39]  Robert H Singer,et al.  Dynamics of transcription and mRNA export. , 2005, Current opinion in cell biology.

[40]  Adriaan B. Houtsmuller,et al.  Macromolecular dynamics in living cell nuclei revealed by fluorescence redistribution after photobleaching , 2001, Histochemistry and Cell Biology.

[41]  Tom Misteli,et al.  Global Nature of Dynamic Protein-Chromatin Interactions In Vivo: Three-Dimensional Genome Scanning and Dynamic Interaction Networks of Chromatin Proteins , 2004, Molecular and Cellular Biology.

[42]  T. Misteli,et al.  High mobility of proteins in the mammalian cell nucleus , 2000, Nature.

[43]  Hiroshi Kimura,et al.  Kinetics of Core Histones in Living Human Cells , 2001, The Journal of cell biology.

[44]  D. Bentley,et al.  Rules of engagement: co-transcriptional recruitment of pre-mRNA processing factors. , 2005, Current opinion in cell biology.

[45]  R. Pego,et al.  Analysis of binding reactions by fluorescence recovery after photobleaching. , 2004, Biophysical journal.

[46]  F. Grosveld,et al.  Inefficient processing impairs release of RNA from the site of transcription , 1999, The EMBO journal.

[47]  Robert H Singer,et al.  Materials and Methods Som Text Figs. S1 to S8 References and Notes Dynamics of Single Mrnps in Nuclei of Living Cells , 2022 .

[48]  Tom Misteli,et al.  Concepts in nuclear architecture , 2005, BioEssays : news and reviews in molecular, cellular and developmental biology.

[49]  E. Manders,et al.  Condensed chromatin domains in the mammalian nucleus are accessible to large macromolecules , 2003, EMBO reports.

[50]  Anthony K. L. Leung,et al.  Nucleolar proteome dynamics , 2005, Nature.

[51]  Tom Misteli,et al.  Dynamic binding of histone H1 to chromatin in living cells , 2000, Nature.

[52]  M. Dundr,et al.  The moving parts of the nucleolus , 2005, Histochemistry and Cell Biology.

[53]  Howard J. Worman,et al.  Nuclear Membrane Dynamics and Reassembly in Living Cells: Targeting of an Inner Nuclear Membrane Protein in Interphase and Mitosis , 1997, The Journal of cell biology.

[54]  David L. Spector,et al.  Nuclear speckles: a model for nuclear organelles , 2003, Nature Reviews Molecular Cell Biology.

[55]  Gabriele Müller,et al.  Analyzing intracellular binding and diffusion with continuous fluorescence photobleaching. , 2003, Biophysical journal.

[56]  J. Gall,et al.  Subnuclear organelles: new insights into form and function. , 2006, Trends in cell biology.

[57]  J. Politz,et al.  Review: movement of mRNA from transcription site to nuclear pores. , 2000, Journal of structural biology.

[58]  D. Spector,et al.  The dynamics of chromosome organization and gene regulation. , 2003, Annual review of biochemistry.

[59]  L. Gunaratnam,et al.  Regulation of ubiquitin ligase dynamics by the nucleolus , 2005, The Journal of cell biology.

[60]  Michael Q. Zhang,et al.  Regulating Gene Expression through RNA Nuclear Retention , 2005, Cell.

[61]  J. Swedlow,et al.  Macromolecular mobility inside the cell nucleus. , 2002, Trends in cell biology.