Epizootic Hemorrhagic Disease Outbreak in a Captive Facility Housing White-Tailed Deer (Odocoileus virginianus), Bison (Bison bison), Elk (Cervus elaphus), Cattle (Bos taurus), and Goats (Capra hircus) in Colorado, USA

Abstract An ungulate research facility in Fort Collins, Colorado, USA, experienced mortality in white-tailed deer (Odocoileus virginianus) because of epizootic hemorrhagic disease virus (EHDV) infection from 20 August 2007 through 26 September 2007. Epizootic hemorrhagic disease virus (EHDV) was detected by reverse transcriptase polymerase chain reaction and virus isolation from the spleen and lung tissues of two white-tailed deer. Virus neutralization tests were performed on pre- and postoutbreak sera from other species maintained in the same facility, including bison (Bison bison), elk (Cervus elaphus), domestic cattle (Bos taurus), and domestic goats (Capra hircus), as well as postoutbreak sera from the surviving white-tailed deer. Serum samples that represented all species in the facility neutralized EHDV-1 and EHDV-2 either before or after the outbreak. The animals that neutralized EHDV-1 did not neutralize EHDV-2. No clinical signs attributable to EHDV infection were noted in any of the species other than the deer during the outbreak. Although experimental EHDV infections have been reported in bison and elk, natural exposures have not been previously documented in these species in North America. The roles that elk, bison, cattle, and goats might play in the epidemiology of EHDV in a close-contact multispecies situation remain unknown.

[1]  D. Stallknecht,et al.  Detection of a novel reassortant epizootic hemorrhagic disease virus (EHDV) in the USA containing RNA segments derived from both exotic (EHDV-6) and endemic (EHDV-2) serotypes. , 2010, The Journal of general virology.

[2]  C. Batten,et al.  Toggenburg Orbivirus, a new bluetongue virus: initial detection, first observations in field and experimental infection of goats and sheep. , 2009, Veterinary microbiology.

[3]  L. Mech,et al.  Survey of Selected Pathogens and Blood Parameters of Northern Yellowstone Elk: Wolf Sanitation Effect Implications , 2007 .

[4]  C. Kato,et al.  An improved, high-throughput method for detection of bluetongue virus RNA in Culicoides midges utilizing infrared-dye-labeled primers for reverse transcriptase PCR. , 2007, Journal of virological methods.

[5]  D. Stallknecht,et al.  Determining Prevalence of Bluetongue and Epizootic Hemorrhagic Disease Viruses in Mule Deer in Arizona (USA) Using Whole Blood Dried on Paper Strips Compared to Serum Analyses , 2006, Journal of wildlife diseases.

[6]  D. Stallknecht,et al.  Genetic variation among epizootic hemorrhagic disease viruses in the southeastern United States: 1978-2001. , 2005, Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases.

[7]  J. Mwanzia,et al.  SEROLOGIC SURVEILLANCE FOR SELECTED VIRAL AGENTS IN CAPTIVE AND FREE-RANGING POPULATIONS OF ARABIAN ORYX (ORYX LEUCORYX) FROM SAUDI ARABIA AND THE UNITED ARAB EMIRATES , 2005, Journal of wildlife diseases.

[8]  E. Milner‐Gulland,et al.  A Serological Survey of Ruminant Livestock in Kazakhstan During Post-Soviet Transitions in Farming and Disease Control , 2004, Acta Veterinaria Scandinavica.

[9]  D. Stallknecht,et al.  EPIZOOTIOLOGY OF AN EPIZOOTIC HEMORRHAGIC DISEASE OUTBREAK IN WEST VIRGINIA , 2004, Journal of wildlife diseases.

[10]  D. Stallknecht,et al.  Hemorrhagic Disease in Kansas: Enzootic Stability Meets Epizootic Disease , 2004, Journal of wildlife diseases.

[11]  S. Boe,et al.  Epizootiology of Hemorrhagic Disease in Mule Deer in Central Arizona , 2004, Journal of wildlife diseases.

[12]  S. Tessaro,et al.  DURATION OF BLUETONGUE VIREMIA IN EXPERIMENTALLY INFECTED AMERICAN BISON , 2001, Journal of wildlife diseases.

[13]  Kazuo Yoshida,et al.  Identification and PCR-Restriction Fragment Length Polymorphism Analysis of a Variant of the Ibaraki Virus from Naturally Infected Cattle and Aborted Fetuses in Japan , 1999, Journal of Clinical Microbiology.

[14]  W. Wilson,et al.  PCR Detection of North American and Central African Isolates of Epizootic Hemorrhagic Disease Virus (EHDV) Based on Genome Segment 10 of EHDV Serotype 1 , 1998, Journal of Clinical Microbiology.

[15]  K. Diem,et al.  Epizootic Hemorrhagic Disease: Analysis of Tissues by Amplification and In Situ Hybridization Reveals Widespread Orbivirus Infection at Low Copy Numbers , 1998, Journal of Virology.

[16]  D. Stallknecht,et al.  HOST DEFENSE RESPONSES ASSOCIATED WITH EXPERIMENTAL HEMORRHAGIC DISEASE IN WHITE-TAILED DEER , 1997, Journal of wildlife diseases.

[17]  D. Stallknecht,et al.  Hemorrhagic Disease in White-tailed Deer in Texas: A Case for Enzootic Stability , 1996, Journal of wildlife diseases.

[18]  D. Stallknecht,et al.  EPIZOOTIC HEMORRHAGIC DISEASE VIRUS AND BLUETONGUE VIRUS SEROTYPE DISTRIBUTION IN WHITE-TAILED DEER IN GEORGIA , 1995, Journal of wildlife diseases.

[19]  B. Osburn,et al.  Experimental Epizootic Hemorrhagic Disease Virus Infection in Calves: Virologic and Serologic Studies , 1994, Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc.

[20]  J. Staats,et al.  Serologic Survey for Selected Microbial Pathogens in Bison from Kansas , 1991, Journal of wildlife diseases.

[21]  E. Shotts,et al.  Bluetongue and epizootic hemorrhagic disease in ruminants in Georgia: survey by serotest and virologic isolation. , 1985, American journal of veterinary research.

[22]  J. Da,et al.  Epidemiology of two orbiviruses in California's native wild ruminants: preliminary report. , 1985 .

[23]  D. McPhee,et al.  Problems in the interpretation of diagnostic tests due to cross-reactions between orbiviruses and broad serological responses in animals. , 1985, Progress in clinical and biological research.

[24]  D. Jessup Epidemiology of two orbiviruses in California's native wild ruminants: preliminary report. , 1985, Progress in clinical and biological research.

[25]  Randall L. Zarnke SEROLOGIC SURVEY FOR SELECTED MICROBIAL PATHOGENS IN ALASKAN WILDLIFE , 1983, Journal of wildlife diseases.

[26]  H. E. Metcalf,et al.  TRANSMISSION OF TWO STRAINS OF EPIZOOTIC HEMORRHAGIC DISEASE VIRUS IN DEER BY Culicoides variipennis , 1977, Journal of wildlife diseases.

[27]  G. Hoff,et al.  EXPERIMENTAL INFECTION IN NORTH AMERICAN ELK WITH EPIZOOTIC HEMORRHAGIC DISEASE VIRUS1 , 1973, Journal of wildlife diseases.

[28]  L. Karstad,et al.  Pathology of epizootic hemorrhagic disease of deer. , 1961, American journal of veterinary research.