Induction of variation in impedance of zebrafish embryos by explicit force feedback control

This paper describes the development of an explicit force control system for exerting a suitable force on the zebrafish chorion while simultaneously measuring its impedance, and presents results demonstrating that the application of an external force leads to significant change in the impedance of a zebrafish embryo. These results offer support for a plausible explanation that activities of pore canals in the chorion are responsible for the observed change in impedance.

[1]  C. Kimmel,et al.  Stages of embryonic development of the zebrafish , 1995, Developmental dynamics : an official publication of the American Association of Anatomists.

[2]  P. Blount,et al.  Mechanosensitive Channels Gated by Membrane Tension , 2008 .

[3]  Stuart T. Smith,et al.  Flexures: Elements of Elastic Mechanisms , 2000 .

[4]  Peter C. Y. Chen,et al.  Force Sensing and Control in Micromanipulation , 2006, IEEE Transactions on Systems, Man, and Cybernetics, Part C (Applications and Reviews).

[5]  X. L. Zhou,et al.  A mechanosensitive ion channel in the yeast plasma membrane. , 1988, Science.

[6]  John J. Craig,et al.  Introduction to Robotics Mechanics and Control , 1986 .

[7]  B. Nelson,et al.  Mechanical analysis of chorion softening in prehatching stages of zebrafish embryos , 2006, IEEE Transactions on NanoBioscience.

[8]  F. Cotelli,et al.  Structure and macromolecular composition of the zebrafish egg chorion , 1996, Zygote.

[9]  Boris Martinac,et al.  Mechanosensitive ion channels: molecules of mechanotransduction , 2004, Journal of Cell Science.

[10]  Christopher M.A. Brett,et al.  Electrochemistry: Principles, Methods, and Applications , 1993 .

[11]  D. Rawson,et al.  Study on fish embryo responses to the treatment of cryoprotective chemicals using impedance spectroscopy , 2006, European Biophysics Journal.

[12]  Peter C. Y. Chen,et al.  A micromanipulation system with dynamic force-feedback for automatic batch microinjection , 2007 .

[13]  John J. Craig Zhu,et al.  Introduction to robotics mechanics and control , 1991 .

[14]  F Sachs,et al.  Quantitative video microscopy of patch clamped membranes stress, strain, capacitance, and stretch channel activation. , 1991, Biophysical journal.

[15]  M. J. Davis,et al.  Stretch-activated single-channel and whole cell currents in vascular smooth muscle cells. , 1992, The American journal of physiology.