Resin extract obtained from Cilician fir (Abies Cilicica) inhibits glucose dependent inflammation in vitro.

Objective The potential anti-inflammatory efficacy of resin extract of Abies cilicica in glucose dependent inflammation and tumor necrosis factor alpha (TNF-a) induced inflammation models was investigated. Its effects on monocyte adhesion, gene expression levels of P-selectin, ICAM-1, VCAM1 and transendothelial migration for the two in vitro models were measured. Also, total flavonoid and total phenolic contents of the extract were determined. Objective Monocyte adhesion tests showed that the extract increased 100% inflammatory effect of TNF-a induced inflammation. On the other hand, it did not change number of adherent monocytes in glucose dependent inflammation model. Although the extract has trigger effect on monocyte adhesion, it did not change migration of leukocytes across ECV304 cells after administration of TNFa on ECV304 cells. The number of migrated monocytes was similar with only TNFa incubation experiment results. However, it significantly decreased monocyte migration in glucose dependent inflammation model. In our both experimental inflammation model, ICAM-1 expression significantly decreased. Although it is known that triggering effect of TNF-a on ICAM-1 expression, the content of of resin extract of A. cilicica prevented this effect. Phenolic antioxidant capacity of the extract are higher than its flavonoid contents.This study provides the first evidence that the extract inhibits glucose dependent inflammation. It may serve as an anti-inflammatory agent in the treatment of chronic inflammation caused by diabetes.

[1]  M. Majeed,et al.  Molecular Action of Inflammation and Oxidative Stress in Hyperglycemic Rats: Effect of Different Concentrations of Pterocarpus marsupiums Extract , 2018, Journal of dietary supplements.

[2]  Yujun Liu,et al.  Hypoglycemic Effects in Alloxan-Induced Diabetic Rats of the Phenolic Extract from Mongolian Oak Cups Enriched in Ellagic Acid, Kaempferol and Their Derivatives , 2018, Molecules.

[3]  W. Vilegas,et al.  Phytochemical study and evaluation of cytotoxicity, mutagenicity, cell cycle kinetics and gene expression of Bauhinia holophylla (Bong.) Steud. in HepG2 cells in vitro , 2018, Cytotechnology.

[4]  Min Wang,et al.  D-chiro-inositol enriched Fagopyrum tataricum (L.) Gaench extract alleviates mitochondrial malfunction and inhibits ER stress/JNK associated inflammation in the endothelium. , 2018, Journal of ethnopharmacology.

[5]  A. Zaid,et al.  Pharmacological and phytochemical screening of Palestinian traditional medicinal plants Erodium laciniatum and Lactuca orientalis , 2018, Journal of complementary & integrative medicine.

[6]  K. Jordan-Sciutto,et al.  Secoisolariciresinol diglucoside is a blood-brain barrier protective and anti-inflammatory agent: implications for neuroinflammation , 2018, Journal of Neuroinflammation.

[7]  J. Lue,et al.  PM2.5-induced oxidative stress increases intercellular adhesion molecule-1 expression in lung epithelial cells through the IL-6/AKT/STAT3/NF-κB-dependent pathway , 2018, Particle and Fibre Toxicology.

[8]  H. Kim,et al.  Anti-inflammatory evaluation of the methanolic extract of Taraxacum officinale in LPS-stimulated human umbilical vein endothelial cells , 2017, BMC Complementary and Alternative Medicine.

[9]  Minh Can Nguyen,et al.  Wound healing activity of Streptocaulon juventas root ethanolic extract , 2017, Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society.

[10]  U. Ghali,et al.  Antidyslipidemic, Anti-Inflammatory, and Antioxidant Activities of Aqueous Leaf Extract of Dioscoreophyllum cumminsii (Stapf) Diels in High-Fat Diet-Fed Rats , 2017, Evidence-based complementary and alternative medicine : eCAM.

[11]  Jennifer Lee,et al.  Pinus densiflora needle supercritical fluid extract suppresses the expression of pro-inflammatory mediators iNOS, IL-6 and IL-1β, and activation of inflammatory STAT1 and STAT3 signaling proteins in bacterial lipopolysaccharide-challenged murine macrophages , 2017, DARU Journal of Pharmaceutical Sciences.

[12]  F. Isik,et al.  Apoptotic Effect of Nigella sativa on Human Lymphoma U937 Cells , 2017, Pharmacognosy magazine.

[13]  F. Aarestrup,et al.  β-Caryophyllene ameliorates the development of experimental autoimmune encephalomyelitis in C57BL/6 mice. , 2017, Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie.

[14]  Jianliang Huang,et al.  Crop Production under Drought and Heat Stress: Plant Responses and Management Options , 2017, Front. Plant Sci..

[15]  T. Farkhondeh,et al.  Attenuation of Oxidative Stress and Inflammation by Portulaca oleracea in Streptozotocin-Induced Diabetic Rats , 2017, Journal of evidence-based complementary & alternative medicine.

[16]  R. Mahmood,et al.  Wound healing potentials of Thevetia peruviana: Antioxidants and inflammatory markers criteria , 2017, Journal of traditional and complementary medicine.

[17]  B. Mustafa,et al.  Phytochemical composition, anti-inflammatory activity and cytotoxic effects of essential oils from three Pinus spp. , 2017, Pharmaceutical biology.

[18]  B. Saka,et al.  Essential Oil Variability and Biological Activities of Tetraclinis articulata (Vahl) Mast. Wood According to the Extraction Time , 2016, Chemistry & biodiversity.

[19]  B. Vasić,et al.  Ethyl Acetate Extract of Origanum vulgare L. ssp. hirtum Prevents Streptozotocin-Induced Diabetes in C57BL/6 Mice. , 2016, Journal of food science.

[20]  G. Sibbald,et al.  Pain management in patients with hidradenitis suppurativa. , 2015, Journal of the American Academy of Dermatology.

[21]  Alejandro Carrasco,et al.  Lavandula angustifolia and Lavandula latifolia Essential Oils from Spain: Aromatic Profile and Bioactivities , 2015, Planta Medica.

[22]  J. Hornecker,et al.  Acute Coronary Syndrome: Out-of-Hospital Evaluation and Management. , 2015, FP essentials.

[23]  R. Moldwin,et al.  Influence of polyunsaturated fatty acids on urologic inflammation , 2015, International Urology and Nephrology.

[24]  Michael B Yaffe,et al.  Kinetics and Role of Plasma Matrix Metalloproteinase-9 Expression in Acute Lung Injury and the Acute Respiratory Distress Syndrome , 2015, Shock.

[25]  E. Altinoz,et al.  THE EFFECT OF SAFFRON (ITS ACTIVE CONSTITUENT, CROCIN) ON THE CARDIOVASCULAR COMPLICATION AND DYSLIPIDEMIA IN STREPTOZOTOCIN INDUCED DIABETIC RATS , 2015, African Journal of Traditional, Complementary and Alternative Medicines.

[26]  C. Denton.  Systemic sclerosis: from pathogenesis to targeted therapy. , 2015, Clinical and experimental rheumatology.

[27]  E. Wagner,et al.  Signalling in inflammatory skin disease by AP-1 (Fos/Jun). , 2015, Clinical and experimental rheumatology.

[28]  A. Yazdi,et al.  The two faces of the inflammasome adaptor ASC in epithelial skin carcinogenesis. , 2015, Clinical and experimental rheumatology.

[29]  A. C. P. Volp,et al.  Adhesion molecules and chemokines; relation to anthropometric, body composition, biochemical and dietary variables. , 2014, Nutricion hospitalaria.

[30]  Lixin Liu,et al.  The role of endothelial cell adhesion molecules P‐selectin, E‐selectin and intercellular adhesion molecule‐1 in leucocyte recruitment induced by exogenous methylglyoxal , 2012, Immunology.

[31]  A. Ariel,et al.  Macrophages, Meta-Inflammation, and Immuno-Metabolism , 2011, TheScientificWorldJournal.

[32]  S. Kersten,et al.  Energy-sensing Factors Coactivator Peroxisome Proliferator-activated Receptor γ Coactivator 1-α (PGC-1α) and AMP-activated Protein Kinase Control Expression of Inflammatory Mediators in Liver , 2011, The Journal of Biological Chemistry.

[33]  I. Tumen,et al.  Wound repair and anti-inflammatory potential of essential oils from cones of Pinaceae: preclinical experimental research in animal models. , 2011, Journal of ethnopharmacology.

[34]  D. Shin,et al.  5-(4-Hydroxy-2,3,5-trimethylbenzylidene) thiazolidine-2,4-dione attenuates atherosclerosis possibly by reducing monocyte recruitment to the lesion , 2011, Experimental and Molecular Medicine.

[35]  Y. Schneider,et al.  Anti-inflammatory effects of dietary phenolic compounds in an in vitro model of inflamed human intestinal epithelium. , 2010, Chemico-biological interactions.

[36]  Y. Erbil,et al.  Preventive Effect of Pentoxifylline on Acute Radiation Damage via Antioxidant and Anti-inflammatory Pathways , 2010, Digestive Diseases and Sciences.

[37]  Xianwen Yang,et al.  Phytochemical and Biological Studies of Abies Species. , 2008 .

[38]  Yun Zhang,et al.  Effect of aspirin plus clopidogrel on inflammatory markers in patients with non-ST-segment elevation acute coronary syndrome. , 2006, Chinese medical journal.

[39]  M. Yavuz,et al.  Antimicrobial Activity of Resins Obtained from the Roots and Stems of Cedrus libani and Abies cilicia , 2002, Applied Biochemistry and Microbiology.

[40]  M. Dığrak,et al.  Antimicrobial activities of several parts of Pinus brutia, Juniperus oxycedrus, Abies cilicia, Cedrus libani and Pinus nigra , 1999, Phytotherapy research : PTR.

[41]  P. Cerutti,et al.  High Glucose Induces Antioxidant Enzymes in Human Endothelial Cells in Culture: Evidence Linking Hyperglycemia and Oxidative Stress , 1996, Diabetes.

[42]  M. Asif.  Chemistry and antioxidant activity of plants containing some phenolic compounds , 2015 .

[43]  F. V. van Schooten,et al.  Dynamic collection and analysis of volatile organic compounds from the headspace of cell cultures , 2015, Journal of breath research.