Enhanced angular domain optical imaging by background scattered light subtraction from a deviated laser source

Imaging structures within a turbid medium using Angular Domain Imaging (ADI) employs angular filter array aligned to a laser source to separate ballistic and quasi-ballistic photons from the highly scattered light by means of angular filtration. The angular filter consists of a high aspect ratio linear array of silicon micromachined tunnels, 51 micron wide by 10mm long with a 0.29 degree acceptance angle. At heavy scattering ratios of >1E7 image detectability declines due to the non-uniform scattered background light fraction still within the acceptance angle. This scattered signal can be separated out by introducing a wedge prism to deviate the laser source where it enters the medium by an angle slightly larger than the acceptance angle. This creates a second image consisting of pure scattering photons with the filtration characteristics of the angular filter, and a pixel by pixel correspondence to the fully scattered illumination emitted from the medium. Experiments used an 808 nm laser diode, collimated to an 8×1 mm line of light, entering a 5cm thick medium with a scattering ratio of > 1E6, with a wedge prism creating a 0.44 degree deviation. Digitally subtracting the deviated scattered signal from the original image significantly reduced the scattered background and enhanced image contrast. We can have about images at least 40 times more of our previous scattering limits. Depending on test phantom object location, the contrast level can be increased from 4% of the total dynamic range to over 50% which results in higher definition and visibility of our micro-scale test structures in the turbid medium.

[1]  Eric L. Miller,et al.  Imaging the body with diffuse optical tomography , 2001, IEEE Signal Process. Mag..

[2]  J. Beuthan,et al.  IR-diaphanoscopy in medicine , 1993, Other Conferences.

[3]  Glenn H. Chapman,et al.  Enhanced angular domain imaging in turbid media using Gaussian line illumination , 2006, SPIE BiOS.

[4]  Glenn H. Chapman,et al.  Angular domain imaging of objects within highly scattering media using silicon micromachined collimating arrays , 2003 .

[5]  Paulman K. Y. Chan,et al.  An Optical Imaging Technique Using Deep Illumination in the Angular Domain , 2007, IEEE Journal of Selected Topics in Quantum Electronics.

[6]  Glenn H. Chapman,et al.  Multispectral angular domain optical tomography in scattering media with argon and diode laser sources , 2007, SPIE BiOS.

[7]  Glenn H. Chapman,et al.  Deep illumination angular domain imaging within highly scattering media enhanced by image processing , 2006, SPIE Optics East.

[8]  A. Santinelli,et al.  Microvessel quantitation in intraductal and early invasive breast carcinomas. , 2000, Analytical and quantitative cytology and histology.

[9]  Robert R. Alfano,et al.  Time-resolved fluorescence and photon migration studies in biomedical and model random media , 1997 .

[10]  B. Tromberg,et al.  Sources of absorption and scattering contrast for near-infrared optical mammography. , 2001, Academic radiology.

[11]  G. G. Stokes "J." , 1890, The New Yale Book of Quotations.

[12]  J. Fujimoto,et al.  Optical Coherence Tomography , 1991, LEOS '92 Conference Proceedings.

[13]  A. Fercher,et al.  Optical coherence tomography - principles and applications , 2003 .

[14]  A F Fercher,et al.  Optical coherence tomography. , 1996, Journal of biomedical optics.

[15]  V. Tuchin Handbook of Optical Biomedical Diagnostics , 2002 .

[16]  Rafael C. González,et al.  Local Determination of a Moving Contrast Edge , 1985, IEEE Transactions on Pattern Analysis and Machine Intelligence.

[17]  Tuan Vo-Dinh,et al.  Biomedical Photonics Handbook , 2003 .

[18]  James G. Fujimoto Optical coherence tomography , 2001 .