The effects of proteins on bacterial attachment to polystyrene.

Bovine serum albumin, gelatin, fibrinogen and pepsin impaired the attachment of a marine pseudomonad to polystyrene Petri dishes, apparently through adsorption on the dish surface. Serum albumin also appeared to affect the bacterial surface. The basic proteins protamine and histone did not markedly inhibit attachment. These findings are discussed in relation to comparative experiments using tissue cells.

[1]  C. E. Zobell.  The Effect of Solid Surfaces upon Bacterial Activity , 1943, Journal of bacteriology.

[2]  Newman Hn.  Microbial films in nature. , 1974 .

[3]  M. Florkin,et al.  Comprehensive biochemistry. Volume 18. Lipid metabolism. , 1970 .

[4]  Ralph Mitchell,et al.  Mechanism of the Initial Events in the Sorption of Marine Bacteria to Surfaces , 1970 .

[5]  A. C. Taylor.  Attachment and spreading of cells in culture. , 1961, Experimental cell research.

[6]  N G Maroudas,et al.  Adhesion and spreading of cells on charged surfaces. , 1975, Journal of theoretical biology.

[7]  G. Martin,et al.  Effects of cell adhesion of the substratum on the growth of chick embryo fibroblasts. , 1974, Experimental cell research.

[8]  M. Fletcher,et al.  An Electron-microscopic Demonstration of an Acidic Polysaccharide Involved in the Adhesion of a Marine Bacterium to Solid Surfaces , 1973 .

[9]  C. Rappaport,et al.  Studies on properties of surfaces required for growth of mammalian cells in synthetic medium. I. The HeLa cell. , 1960, Experimental cell research.

[10]  J. Overbeek,et al.  Theory of the stabilization of dispersions by adsorbed macromolecules. II. Interaction between two flat particles , 1971 .

[11]  L. Weiss.  STUDIES ON CELLULAR ADHESION IN TISSUE CULTURE. VII. SURFACE ACTIVITY AND CELL DETACHMENT. , 1959, Experimental cell research.

[12]  H. Heukelekian,et al.  Relation between Food Concentration and Surface for Bacterial Growth , 1940, Journal of bacteriology.

[13]  C. Rappaport.  Studies on properties of surfaces required for growth of mammalian cells in synthetic medium. II. The monkey kidney cell. , 1960, Experimental cell research.

[14]  L. Weiss,et al.  Studies on cellular adhesion in tissue culture. I. The effect of serum. , 1959, Experimental cell research.

[15]  N. Maroudas.  Polymer exclusion, cell adhesion and membrane fusion , 1975, Nature.

[16]  C. E. Zobell,et al.  OBSERVATIONS ON THE MULTIPLICATION OF BACTERIA IN DIFFERENT VOLUMES OF STORED SEA WATER AND THE INFLUENCE OF OXYGEN TENSION AND SOLID SURFACES , 1936 .

[17]  J. M. Shewan,et al.  A DETERMINATIVE SCHEME FOR THE IDENTIFICATION OF CERTAIN GENERA OF GRAM‐NEGATIVE BACTERIA, WITH SPECIAL REFERENCE TO THE PSEUDOMONADACEAE , 1960 .

[18]  N. Maroudas.  Chemical and Mechanical Requirements for Fibroblast Adhesion , 1973, Nature.

[19]  N. Kovacs.  Identification of Pseudomonas pyocyanea by the Oxidase Reaction , 1956, Nature.

[20]  E. Leifson,et al.  THE TAXONOMIC SIGNIFICANCE OF FERMENTATIVE VERSUS OXIDATIVE METABOLISM OF CARBOHYDRATES BY VARIOUS GRAM NEGATIVE BACTERIA , 1953, Journal of bacteriology.

[21]  P. Meadows.  Attachment of Marine- and Fresh-water Bacteria to Solid Surfaces , 1965, Nature.

[22]  L. Weiss.  Cellular locomotive pressure in relation to initial cell contacts. , 1964, Journal of theoretical biology.

[23]  H. Newman.  Microbial films in nature. , 1974, Microbios.