Chemiluminescence immunoassay using magnetic nanoparticles with targeted inhibition for the determination of ochratoxin A.

In this work, a chemiluminescence (CL) immunoassay with targeted inhibition was developed for the determination of toxins in food products. For sample treatment, amine-functionalized magnetic nanoparticles (MNPs) were synthesized to extract target molecules, and horseradish peroxidase (HRP) tagged on an antibody was used as a label for CL reaction. In particular, amine-targeted inhibition using aldehyde, i.e., specifically capping the amine with an alkyl group, was developed for a non-specific extraction platform to lower background and improve signal-to-background ratio. For demonstration, ochratoxin A (OTA) was determined in rice using a lab-built drop-type chemiluminescence (DCL) system with luminol-H2O2 reagent. The obtained limit of detection was 1.39 pg mL(-1), which was about 7.3 times better than that of ELISA. Recovery of the method in the range of 87-99% was observed, which was compared with ELISA.

[1]  H. Ju,et al.  Label-free surface-enhanced Raman spectroscopy for sensitive DNA detection by DNA-mediated silver nanoparticle growth. , 2013, Analytical chemistry.

[2]  C. Lino,et al.  A review on ochratoxin A occurrence and effects of processing of cereal and cereal derived food products. , 2010, Food microbiology.

[3]  H. B. Lim,et al.  Chemiluminescence system for direct determination and mapping of ultra-trace metal impurities on a silicon wafer. , 2010, The Analyst.

[4]  J. M. Soriano,et al.  Rapid determination of ochratoxin A in cereals and cereal products by liquid chromatography. , 2004, Journal of chromatography. A.

[5]  Norbert Jakubowski,et al.  Comparison of ICP-MS and photometric detection of an immunoassay for the determination of ochratoxin A in wine , 2010 .

[6]  Arunas Ramanavicius,et al.  Magnetic gold nanoparticles in SERS-based sandwich immunoassay for antigen detection by well oriented antibodies. , 2013, Biosensors & bioelectronics.

[7]  H. B. Lim,et al.  Application of magnetic and core-shell nanoparticles to determine enrofloxacin and its metabolite using laser induced fluorescence microscope. , 2013, Analytica chimica acta.

[8]  A. Abdou,et al.  Ochratoxicosis in Broiler Chickens: Functional and Histological Changes in Target Organs , 2008 .

[9]  L. Bullerman,et al.  Stability of mycotoxins during food processing. , 2007, International journal of food microbiology.

[10]  P. Schieberle,et al.  Quantification of ochratoxin A in foods by a stable isotope dilution assay using high-performance liquid chromatography-tandem mass spectrometry. , 2004, Journal of chromatography. A.

[11]  Metal/dye-doped core-shell silica nanoparticles for potential use in bioassay , 2013 .

[12]  H. B. Lim,et al.  Direct and simultaneous determination of Co and Cu on a silicon wafer using a chemiluminescence system. , 2011, The Analyst.

[13]  Nuo Duan,et al.  Aptamer-functionalized magnetic nanoparticle-based bioassay for the detection of ochratoxin A using upconversion nanoparticles as labels. , 2011, The Analyst.

[14]  A. Roland,et al.  Analysis of ochratoxin A in grapes, musts and wines by LC-MS/MS: first comparison of stable isotope dilution assay and diastereomeric dilution assay methods. , 2014, Analytica chimica acta.

[15]  A. Lehmann,et al.  Determination of ochratoxin A in beer by LC–MS/MS ion trap detection , 2007 .

[16]  S. Bamrungsap,et al.  Rapid and sensitive lateral flow immunoassay for influenza antigen using fluorescently-doped silica nanoparticles , 2013, Microchimica Acta.

[17]  E. Meulenberg.  Immunochemical Methods for Ochratoxin A Detection: A Review , 2012, Toxins.

[18]  Meihua Yang,et al.  Molecularly imprinted polymer-based solid phase clean-up for analysis of ochratoxin A in ginger and LC-MS/MS confirmation , 2013 .

[19]  H. B. Lim,et al.  Sample treatment platform using nanoparticles to determine salinomycin in flesh and meat. , 2014, Food chemistry.

[20]  H. Lim,et al.  Multicore magnetic nanoparticles (MMNPs) doped with Cs and FITC for the determination of biomarker in serum using ICP-MS. , 2014, Analytical chemistry.

[21]  H. B. Lim,et al.  Characterization and analytical application of surface modified magnetic nanoparticles , 2010 .

[22]  Huimin Zhao,et al.  Electrochemiluminescence immunosensor for highly sensitive detection of 8-hydroxy-2'-deoxyguanosine based on carbon quantum dot coated Au/SiO2 core-shell nanoparticles. , 2015, Talanta.

[23]  D. Neri,et al.  Site-specific chemical modification of antibody fragments using traceless cleavable linkers , 2013, Nature Protocols.

[24]  R. Niessner,et al.  Quantum dot loaded liposomes as fluorescent labels for immunoassay. , 2013, Analytical chemistry.

[25]  Hongyuan Chen,et al.  Versatile immunosensor using CdTe quantum dots as electrochemical and fluorescent labels. , 2007, Analytical chemistry.

[26]  Wenlu Song,et al.  Simultaneous determination of bisphenol A, aflatoxin B1, ochratoxin A, and patulin in food matrices by liquid chromatography/mass spectrometry. , 2013, Rapid communications in mass spectrometry : RCM.