Distribution of the cell substratum attachment (CSAT) antigen on myogenic and fibroblastic cells in culture

Previous studies (Neff et al., 1982, J. Cell. Biol. 95:654-666; Decker et al., 1984. J. Cell. Biol. 99:1388-1404) have described a monoclonal antibody (CSAT Mab) directed against a complex of three integral membrane glycoproteins of 120,000-160,000 mol wt (CSAT antigen [ag]) involved in the cell matrix adhesion of myoblasts and fibroblasts. In localization studies on fibroblasts presented here, CSAT ag has a discrete, well-organized distribution pattern. It co-aligns with portions of stress fibers and is enriched at the periphery of, but not directly beneath vinculin-rich focal contacts. In this last location, it co-distributes with fibronectin, consistent with the suggestion that the CSAT ag participates in the mechanism by which fibroblasts attach to fibronectin. In prefusion myoblasts, which are rapidly detached by CSAT Mab, CSAT ag is distributed diffusely as are vinculin, laminin, and fibronectin. After fusion, myotubes become more difficult to detach with CSAT Mab. The CSAT ag and vinculin are organized in a much more discrete pattern on the myotube surface, becoming enriched at microfilament bundle termini and in lateral lamellae which appear to attach myotubes to the substratum. These results suggest that the organization of CSAT ag-adhesive complexes on the surface of myogenic cells can affect the stability of their adhesive contacts. We conclude from the sum of the studies presented that, in both myogenic and fibroblastic cells, the CSAT ag is localized in sites expected of a surface membrane mediator of cell adhesion to extracelluon of CSAT ag- adhesive complexes on the surface of myogenic cells can affect the stability of their adhesive contacts. We conclude from the sum of the studies presented that, in both myogenic and fibroblastic cells, the CSAT ag is localized in sites expected of a surface membrane mediator of cell adhesion to extracellular matrix. The results from studies that use fibroblasts in particular suggest the involvement of CSAT ag in the adhesion of these cells to fibronectin.

[1]  W. T. Chen,et al.  Development of cell surface linkage complexes in cultured fibroblasts , 1985, The Journal of cell biology.

[2]  A. Horwitz,et al.  A monoclonal antibody identifies a glycoprotein complex involved in cell-substratum adhesion. , 1985, Experimental cell research.

[3]  E. Ruoslahti,et al.  Identification and isolation of a 140 kd cell surface glycoprotein with properties expected of a fibronectin receptor , 1985, Cell.

[4]  C. Damsky,et al.  Cleavage of a 135 kD cell surface glycoprotein correlates with loss of fibroblast adhesion to fibronectin. , 1985, Experimental cell research.

[5]  A. Horwitz,et al.  Adhesive multiplicity in the interaction of embryonic fibroblasts and myoblasts with extracellular matrices , 1984, The Journal of cell biology.

[6]  M. Chiquet,et al.  Chick myotendinous antigen. I. A monoclonal antibody as a marker for tendon and muscle morphogenesis , 1984, The Journal of cell biology.

[7]  C. Damsky,et al.  Integral Membrane Glycoproteins in Cell-Cell and Cell-Substratum Adhesion , 1984 .

[8]  N. Neff,et al.  Adhesion-Related Integral Membrane Glycoproteins Identified by Monoclonal Antibodies , 1984 .

[9]  A. Chapman.  Characterization of a 140Kd cell surface glycoprotein involved in myoblast adhesion , 1984, Journal of cellular biochemistry.

[10]  R. Ivatt.  The Biology of Glycoproteins , 1984, Springer US.

[11]  L. Connell,et al.  A new protein of adhesion plaques and ruffling membranes , 1983, The Journal of cell biology.

[12]  E. Ruoslahti,et al.  Synthetic peptide with cell attachment activity of fibronectin. , 1983, Proceedings of the National Academy of Sciences of the United States of America.

[13]  W. Birchmeier,et al.  New surface component of fibroblast's focal contacts identified by a monoclonal antibody , 1982, Cell.

[14]  N. Neff,et al.  A Monoclonal Antibody Detaches Muscle from Extracellular Matrices Embryonic Skeletal , 2003 .

[15]  W. T. Chen,et al.  Immunoelectron microscopic studies of the sites of cell-substratum and cell-cell contacts in cultured fibroblasts , 1982, The Journal of cell biology.

[16]  D. Gottlieb,et al.  Monoclonal Antibodies Which Alter the Morphology of Cultured Chick Myogenic Cells , 1982, Journal of cellular biochemistry.

[17]  C. Damsky,et al.  Membrane glycoproteins involved in cell--substratum adhesion. , 1981, Proceedings of the National Academy of Sciences of the United States of America.

[18]  C. Damsky,et al.  Manipulation of cell-cell and cell-substratum interactions in mouse mammary tumor epithelial cells using broad spectrum antisera , 1981, The Journal of cell biology.

[19]  I. Singer,et al.  A transmembrane relationship between fibronectin and vinculin (130 kd protein): Serum modulation in normal and transformed hamster fibroblasts , 1981, Cell.

[20]  W. N. Burnette,et al.  "Western blotting": electrophoretic transfer of proteins from sodium dodecyl sulfate--polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A. , 1981, Analytical biochemistry.

[21]  B. Geiger,et al.  Vinculin, an intracellular protein localized at specialized sites where microfilament bundles terminate at cell membranes. , 1980, Proceedings of the National Academy of Sciences of the United States of America.

[22]  W. Birchmeier,et al.  Corrugated attachment membrane in WI-38 fibroblasts: alternating fibronectin fibers and actin-containing focal contacts. , 1980, Proceedings of the National Academy of Sciences of the United States of America.

[23]  L. Rohrschneider.  Adhesion plaques of Rous sarcoma virus-transformed cells contain the src gene product. , 1980, Proceedings of the National Academy of Sciences of the United States of America.

[24]  K. Weber,et al.  Cell-to-substratum contacts in living cells: a direct correlation between interference-reflexion and indirect-immunofluorescence microscopy using antibodies against actin and alpha-actinin. , 1979, Journal of cell science.

[25]  J. Heath,et al.  Cell to substratum contacts of chick fibroblasts and their relation to the microfilament system. A correlated interference-reflexion and high-voltage electron-microscope study. , 1978, Journal of cell science.

[26]  C. S. Izzard,et al.  Cell-to-substrate contacts in living fibroblasts: an interference reflexion study with an evaluation of the technique. , 1976, Journal of cell science.

[27]  U. K. Laemmli,et al.  Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4 , 1970, Nature.