The influence of powdered coconut water (ACP-318®) in in vitro maturation of canine oocytes.

The objective of this study was to determine the influence of powdered coconut water (ACP-318(®)) diluted in high glucose (11.0 mM) TCM199 in the achievement of nuclear in vitro maturation (IVM) of canine oocytes. Cumulus oocyte complexes (COCs) (n = 632) were randomly allocated into three experimental groups named as group 1 (control group), group 2 (5% powdered coconut water) and group 3 (10% powdered coconut water). The percentage of meiotic resumption (MR) (GVBD to MII) was 39.1% (81/207), 50.2% (108/215) and 46.6% (98/210) for groups 1, 2 and 3 respectively (p < 0.05). There were no differences in MR rates among groups 2 and 3. The medium with ACP-318(®) slightly enhanced the nuclear maturation of canine oocytes when a comparison was established with rates of maturation exhibited by oocytes in the experimental group 1 without ACP-318(®) (p < 0.05). The results suggest that oocytes' nuclear morphology integrity and meiosis achievement were positively influenced when exposed to high glucose TCM199 supplemented with 5% powdered coconut water. Further investigation must be performed for a better understanding of powdered coconut water influence in cellular events during IVM of dog oocytes.

[1]  M. Miranda,et al.  The use of coconut water solution ( Cocos nucifera ) as a holding medium for immature bovine oocytes for in vitro embryo production , 2007 .

[2]  A. R. Silva,et al.  Use of the powdered coconut water (ACP-106 ® ) for cryopreservation of canine spermatozoa , 2006 .

[3]  H. Khan,et al.  Effect of L-ascorbic Acid on Nili Ravi Buffalo Oocytes During in vitro Maturation , 2006 .

[4]  C. Barros,et al.  Effect of human chorionic gonadotrophin supplementation during different culture periods on in vitro maturation of canine oocytes. , 2005, Theriogenology.

[5]  Romeu Vianni,et al.  Características físicas e químicas da água de coco anão verde e anão vermelho em diferentes estádios de maturação , 2005 .

[6]  C. Pessoa.  Development of goat primordial follicles after in vitro culture of ovarian tissue in Minimal Essential Medium supplemented with coconut water , 2005 .

[7]  T. Otoi,et al.  Influence of maturation culture period on the development of canine oocytes after in vitro maturation and fertilization. , 2004, Reproduction, nutrition, development.

[8]  E. R. Andrade,et al.  Survival and growth of goat primordial follicles after in vitro culture of ovarian cortical slices in media containing coconut water. , 2004, Animal reproduction science.

[9]  M. Fu,et al.  Exposure to L-ascorbic acid or alpha-tocopherol facilitates the development of porcine denuded oocytes from metaphase I to metaphase II and prevents cumulus cells from fragmentation. , 2004, Reproduction in domestic animals = Zuchthygiene.

[10]  Lucila Carboneiro dos Santos,et al.  Embryonic development of in vitro matured and in vitro fertilized dog oocytes , 2004, Molecular reproduction and development.

[11]  G. Luvoni,et al.  Meiosis resumption of canine oocytes cultured in the isolated oviduct. , 2003, Reproduction in domestic animals = Zuchthygiene.

[12]  J. L. Rodrigues,et al.  Meiotic response of in vitro matured canine oocytes under different proteins and heterologous hormone supplementation. , 2003, Reproduction in domestic animals = Zuchthygiene.

[13]  A. Agarwal,et al.  Vitamin C and vitamin E supplementation reduce oxidative stress-induced embryo toxicity and improve the blastocyst development rate. , 2002, Fertility and sterility.

[14]  D. Gardner,et al.  Addition of ascorbate during cryopreservation stimulates subsequent embryo development. , 2002, Human reproduction.

[15]  E. R. Andrade,et al.  Evaluation of saline and coconut water solutions in the preservation of sheep preantral follicles in situ , 2002 .

[16]  G. Shui,et al.  An investigation of antioxidant capacity of fruits in Singapore markets , 2002 .

[17]  E. R. Andrade,et al.  Effects of storage time and temperature on atresia of goat ovarian preantral follicles held in M199 with or without indole-3-acetic acid supplementation. , 2001, Theriogenology.

[18]  M. F. Smith,et al.  Role of ascorbic acid in promoting follicle integrity and survival in intact mouse ovarian follicles in vitro. , 2001, Reproduction.

[19]  J. R. Figueiredo,et al.  Effect of coconut water and Braun-Collins solutions at different temperatures and incubation times on the morphology of goat preantral follicles preserved in vitro. , 2000, Theriogenology.

[20]  T. Otoi,et al.  Oocyte diameter in relation to meiotic competence and sperm penetration. , 2000, Theriogenology.

[21]  W. Farstad.  Current state in biotechnology in canine and feline reproduction. , 2000, Animal reproduction science.

[22]  T. Greve,et al.  Effect of administering a crude equine gonadotrophin preparation to mares on follicular development, oocyte recovery rate and oocyte maturation in vivo. , 2000, Journal of reproduction and fertility.

[23]  B. Durrant,et al.  In vitro maturation of domestic dog oocytes cultured in advanced preantral and early antral follicles. , 1998, Theriogenology.

[24]  T. Tadokoro,et al.  Nutrient composition of kopyor coconuts (Cocos nucifera L.) , 1996 .

[25]  B. Brackett,et al.  Improvement in bovine embryo production in vitro by glutathione‐containing culture media , 1996, Molecular reproduction and development.

[26]  Y. Combarnous,et al.  Effect of indole-3-acetic acid (plant auxin) on the preservation at 15 °c of boar semen for artificial insemination , 1996 .

[27]  A. Meister.  Glutathione-ascorbic acid antioxidant system in animals. , 1994, The Journal of biological chemistry.

[28]  A. Bode,et al.  Biology of free radical scavengers: an evaluation of ascorbate , 1993, FASEB journal : official publication of the Federation of American Societies for Experimental Biology.

[29]  G. Buettner.  The pecking order of free radicals and antioxidants: lipid peroxidation, alpha-tocopherol, and ascorbate. , 1993, Archives of biochemistry and biophysics.

[30]  M. Levine.  New concepts in the biology and biochemistry of ascorbic acid. , 1986, The New England journal of medicine.

[31]  P. Raven,et al.  Biology of plants , 1971 .

[32]  M. Jain,et al.  INTERACTION EFFECTS OF GLUCOSE AND AUXINS IN ROOTING ETIOLATED STEM SEGMENTS OF SALIX TETRASPERMA , 1971 .

[33]  Whittingham Dg.  Culture of mouse ova. , 1971 .